Solid-phase radioimmunoassay for human placental lactogen in serum.
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Abstract We report a solid-phase radioimmunoassay for human placental lactogen, in which antibody-coated polystyrene tubes and polyurethane sponges are used. Incubation time and temperature, ionic strength of buffer, and nonspecific adsorption have been evaluated for optimum conditions required for routine clinical assays. The validity of the assay technique has been ascertained by its specificity, reproducibility, and recovery. The sensitivity of this method is 0.4 ng of placental lactogen per assay tube.
1991 ◽
Vol 149
(2)
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pp. 295-304
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1968 ◽
Vol 20
(01/02)
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pp. 001-006
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1986 ◽
Vol 13
(1)
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pp. 27-33
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1991 ◽
Vol 266
(20)
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pp. 13397-13404
1989 ◽
Vol 264
(16)
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pp. 9215-9219
1984 ◽
Vol 259
(23)
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pp. 14642-14646
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