Cloning and heterologous expression of the violacein biosynthesis gene cluster fromChromobacterium violaceum

1991 ◽  
Vol 22 (6) ◽  
pp. 355-358 ◽  
Author(s):  
John M. Pemberton ◽  
Karen M. Vincent ◽  
Robert J. Penfold
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

Cloning and Heterologous Expression of the Aurachin RE Biosynthesis Gene Cluster Afford a New Cytochrome P450 for Quinoline N-Hydroxylation

ChemBioChem ◽  
2013 ◽  
Vol 14 (9) ◽  
pp. 1085-1093 ◽  
Author(s):  
Wataru Kitagawa ◽  
Taro Ozaki ◽  
Taiki Nishioka ◽  
Yoshiaki Yasutake ◽  
Miyako Hata ◽  
...  
Keyword(s):  
Cytochrome P450 ◽  
Heterologous Expression ◽  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Development of a Genetic System for Combinatorial Biosynthesis of Lipopeptides in Streptomyces fradiae and Heterologous Expression of the A54145 Biosynthesis Gene Cluster

2010 ◽  
Vol 76 (20) ◽  
pp. 6877-6887 ◽  
Author(s):  
Dylan C. Alexander ◽  
Jessica Rock ◽  
Xiaowei He ◽  
Paul Brian ◽  
Vivian Miao ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Glutamic Acid ◽  
Gene Cluster ◽  
Genetic System ◽  
Combinatorial Biosynthesis ◽  
Streptomyces Fradiae ◽  
Bac Clone ◽  
Biosynthesis Gene Cluster ◽  
Streptomyces Ambofaciens ◽  
Biosynthesis Gene

ABSTRACT A54145 factors are calcium-dependent lipopeptide antibiotics produced by Streptomyces fradiae NRRL 18160. A54145 is structurally related to the clinically important daptomycin, and as such may be a useful scaffold for the development of a novel lipopeptide antibiotic. We developed methods to genetically manipulate S. fradiae by deletion mutagenesis and conjugal transfer of plasmids from Escherichia coli. Cloning the complete pathway on a bacterial artificial chromosome (BAC) vector and the construction of ectopic trans-complementation with plasmids utilizing the φC31 or φBT1 site-specific integration system allowed manipulation of A54145 biosynthesis. The BAC clone pDA2002 was shown to harbor the complete A54145 biosynthesis gene cluster by heterologous expression in Streptomyces ambofaciens and Streptomyces roseosporus strains in yields of >100 mg/liter. S. fradiae mutants defective in LptI methyltransferase function were constructed, and they produced only A54145 factors containing glutamic acid (Glu12), at the expense of factors containing 3-methyl-glutamic acid (3mGlu12). This provided a practical route to produce high levels of pure Glu12-containing lipopeptides. A suite of mutant strains and plasmids was created for combinatorial biosynthesis efforts focused on modifying the A54145 peptide backbone to generate a compound with daptomycin antibacterial activity and activity in Streptococcus pneumoniae pulmonary infections.

scholarly journals Identification and Heterologous Expression of the Chaxamycin Biosynthesis Gene Cluster from Streptomyces leeuwenhoekii

2015 ◽  
Vol 81 (17) ◽  
pp. 5820-5831 ◽  
Author(s):  
Jean Franco Castro ◽  
Valeria Razmilic ◽  
Juan Pablo Gomez-Escribano ◽  
Barbara Andrews ◽  
Juan A. Asenjo ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Gene Cluster ◽  
Mutational Analysis ◽  
Streptomyces Coelicolor ◽  
Atacama Desert ◽  
Biosynthesis Gene Cluster ◽  
Content Type ◽  
Biosynthesis Gene ◽  
Viable Approach

ABSTRACTStreptomyces leeuwenhoekii, isolated from the hyperarid Atacama Desert, produces the new ansamycin-like compounds chaxamycins A to D, which possess potent antibacterial activity and moderate antiproliferative activity. We report the development of genetic tools to manipulateS. leeuwenhoekiiand the identification and partial characterization of the 80.2-kb chaxamycin biosynthesis gene cluster, which was achieved by both mutational analysis in the natural producer and heterologous expression inStreptomyces coelicolorA3(2) strain M1152. Restoration of chaxamycin production in a nonproducing ΔcxmKmutant (cxmKencodes 3-amino-5-hydroxybenzoic acid [AHBA] synthase) was achieved by supplementing the growth medium with AHBA, suggesting that mutasynthesis may be a viable approach for the generation of novel chaxamycin derivatives.

An alginate-like exopolysaccharide biosynthesis gene cluster involved in biofilm aerial structure formation by Pseudomonas alkylphenolia

2014 ◽  
Vol 98 (9) ◽  
pp. 4137-4148 ◽  
Author(s):  
Kyoung Lee ◽  
Eun Jin Lim ◽  
Keun Soo Kim ◽  
Shir-Ly Huang ◽  
Yaligara Veeranagouda ◽  
...  
Keyword(s):  
Structure Formation ◽  
Gene Cluster ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Exopolysaccharide Biosynthesis

Response of microcystin biosynthesis and its biosynthesis gene cluster transcription in Microcystis aeruginosa on electrochemical oxidation

2018 ◽  
Vol 40 (27) ◽  
pp. 3593-3601 ◽  
Author(s):  
Yu Gao ◽  
Kazuya Shimizu ◽  
Chie Amano ◽  
Xin Wang ◽  
Thanh Luu Pham ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Electrochemical Oxidation ◽  
Microcystis Aeruginosa ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene

scholarly journals Characterization of the Biosynthesis Gene Cluster for the Pyrrole Polyether Antibiotic Calcimycin (A23187) inStreptomyces chartreusisNRRL 3882

2010 ◽  
Vol 55 (3) ◽  
pp. 974-982 ◽  
Author(s):  
Qiulin Wu ◽  
Jingdan Liang ◽  
Shuangjun Lin ◽  
Xiufen Zhou ◽  
Linquan Bai ◽  
...  
Keyword(s):  
Gene Cluster ◽  
Mutational Analysis ◽  
Divalent Cations ◽  
Biological Effects ◽  
Biosynthetic Gene Cluster ◽  
Type I ◽  
Biosynthesis Gene Cluster ◽  
Biosynthesis Gene ◽  
Hydroxyanthranilic Acid

ABSTRACTThe pyrrole polyether antibiotic calcimycin (A23187) is a rare ionophore that is specific for divalent cations. It is widely used as a biochemical and pharmacological tool because of its multiple, unique biological effects. Here we report on the cloning, sequencing, and mutational analysis of the 64-kb biosynthetic gene cluster fromStreptomyces chartreusisNRRL 3882. Gene replacements confirmed the identity of the gene cluster, andin silicoanalysis of the DNA sequence revealed 27 potential genes, including 3 genes for the biosynthesis of the α-ketopyrrole moiety, 5 genes that encode modular type I polyketide synthases for the biosynthesis of the spiroketal ring, 4 genes for the biosynthesis of 3-hydroxyanthranilic acid, anN-methyltransferase tailoring gene, a resistance gene, a type II thioesterase gene, 3 regulatory genes, 4 genes with other functions, and 5 genes of unknown function. We propose a pathway for the biosynthesis of calcimycin and assign the genes to the biosynthesis steps. Our findings set the stage for producing much desired calcimycin derivatives using genetic modification instead of chemical synthesis.

scholarly journals Enhancement of the Diversity of Polyoxins by a Thymine-7-Hydroxylase Homolog outside the Polyoxin Biosynthesis Gene Cluster

2010 ◽  
Vol 76 (21) ◽  
pp. 7343-7347 ◽  
Author(s):  
Changming Zhao ◽  
Tingting Huang ◽  
Wenqing Chen ◽  
Zixin Deng
Keyword(s):  
Gene Cluster ◽  
Carbon Skeleton ◽  
Open Reading Frame ◽  
Streptomyces Avermitilis ◽  
Biosynthesis Gene Cluster ◽  
Reading Frame ◽  
Biosynthesis Gene ◽  
Pyrimidine Moiety

ABSTRACT Polyoxins consist of 14 structurally variable components which differentiate at three branch sites of the carbon skeleton. Open reading frame (ORF) SAV_4805 of Streptomyces avermitilis, showing similarity to thymine-7-hydroxylase, was proved to enhance the diversity of polyoxins at the C-5 site of the 1-(5′-amino-5′-deoxy-β-d-allofuranuronosyl) pyrimidine moiety.

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