Prevalence ofCampylobacter jejuni, Salmonella typhimurium, and avianParamyxovirus type 1 (PMV-1) in pigeons from different regions in Croatia

2003 ◽  
Vol 49 (4) ◽  
pp. 303-313 ◽  
Author(s):  
Marija Vučemilo ◽  
Ksenija Vlahović ◽  
Alenka Dovč ◽  
Jasmina MuŽinić ◽  
Marina Pavlak ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Ofcampylobacter Jejuni

sRNA STnc150 is involved in virulence regulation of Salmonella Typhimurium by targeting fimA mRNA

2021 ◽  
Vol 368 (18) ◽  
Author(s):  
Jing Li ◽  
Na Li ◽  
Chengcheng Ning ◽  
Yun Guo ◽  
Chunhui Ji ◽  
...  
Keyword(s):  
Salmonella Typhimurium ◽  
Target Genes ◽  
Reporter System ◽  
Stem Loop ◽  
Viral Loads ◽  
Virulence Regulation ◽  
Stem Loop Structure ◽  
Complementary Strain

ABSTRACT Small RNAs (sRNAs) are essential virulent regulators in Salmonella typhimurium (STM). To explore the role of sRNA STnc150 in regulating STM virulence, we constructed a STnc150 deletion strain (ΔSTnc150) and its complementary strain (ΔSTnc150/C). Then, we compared their characteristics to their original parent strain experimentally, identified the target genes of STnc150 and determined the expression levels of target genes. The results showed that the ΔSTnc150 strain exhibited delayed biofilm formation, enhanced adhesion to macrophages, significantly reduced LD50, increased liver and spleen viral loads and more vital pathological damaging ability than its parent and complementary strains. Further, bioinformatics combined with the bacterial dual plasmid reporter system confirmed that the bases 72–88 of STnc150 locating at the secondary stem-loop structure of the STnc150 are complementary with the bases 1–19 in the 5′-terminal of fimA mRNA of the type 1 fimbriae subunit. Western blot analysis showed that fimA protein level was increased in STnc150 strain compared with its parent and complementary strains. Together, this study suggested that STnc150 can down-regulate STM fimA expression at the translation level, which provided insights into the regulatory mechanisms of sRNAs in virulence of STM.

scholarly journals Salmonella typhimurium infection halts development of type 1 diabetes in NOD mice

2004 ◽  
Vol 34 (11) ◽  
pp. 3246-3256 ◽  
Author(s):  
Paola Zaccone ◽  
Tim Raine ◽  
St�phane Sidobre ◽  
Mitchell Kronenberg ◽  
Pietro Mastroeni ◽  
...  
Keyword(s):  
Type 1 Diabetes ◽  
Salmonella Typhimurium ◽  
Nod Mice
Sign in / Sign up

Export Citation Format

Share Document