Histamine-releasing activity of mouse spleen cell culture supernatants

1989 ◽  
Vol 27 (1-2) ◽  
pp. 46-48
Author(s):  
J. Wyczóŀkowska ◽  
Z. Suŀowska ◽  
E. Brzenińska-Bŀaszczyk
Keyword(s):  
Cell Culture ◽  
Spleen Cell ◽  
Mouse Spleen ◽  
Mouse Spleen Cell ◽  
Spleen Cell Culture ◽  
Culture Supernatants

Initiation of the Primary Immune Response to Sheep Red Blood Cells in the Dissociated Mouse Spleen Cell Culture

1971 ◽  
Vol 15 (6) ◽  
pp. 493-498
Author(s):  
Takushi Tadakuma ◽  
Keiichi Watanabe ◽  
Takashi Mitsuma ◽  
Kazuhisa Saito
Keyword(s):  
Cell Culture ◽  
Immune Response ◽  
Red Blood Cells ◽  
Spleen Cell ◽  
Blood Cells ◽  
Primary Immune Response ◽  
Mouse Spleen ◽  
Mouse Spleen Cell ◽  
Spleen Cell Culture ◽  
Sheep Red Blood Cells

scholarly journals The Effects of Maternal Smoking on Pregnancy and Offspring: Possible Role for EGF?

2021 ◽  
Vol 9 ◽  
Author(s):  
Hamed Janbazacyabar ◽  
Marthe van Daal ◽  
Thea Leusink-Muis ◽  
Ingrid van Ark ◽  
Johan Garssen ◽  
...  
Keyword(s):  
Cell Culture ◽  
Immune Responses ◽  
Spleen Cell ◽  
Cigarette Smoke ◽  
Mammary Glands ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Positive Correlation ◽  
Spleen Cell Culture ◽  
Culture Supernatants

Cigarette smoke exposure during pregnancy and lactation is associated with adverse pregnancy outcomes. Here, we investigated the effects of maternal smoke exposure on pregnancy and offspring immunity and explored whether, epidermal growth factor (EGF), an important growth-promoting factor in human colostrum and milk, might be a possible missing link in maternal smoke exposure and changes in infants’ immune responses. Pregnant BALB/c mice were exposed to either cigarette smoke or air during gestation and lactation, and effects on pulmonary inflammation in dams and immune responses in offspring were examined. Maternal smoke exposure increased airway hyperresponsiveness and accumulation of inflammatory cells in the lungs of pregnant dams compared to non-pregnant dams. The E-cadherin protein expression was reduced in mammary glands of cigarette smoke-exposed pregnant dams. EGF levels were higher in mammary glands and serum of smoke-exposed pregnant dams compared to air-exposed pregnant dams. Offspring from cigarette smoke-exposed dams exhibited elevated levels of IL-17A, MCP-1, IL-22, and IL-13 in anti-CD3 stimulated spleen cell culture supernatants. EGF levels were also increased in serum of offspring from smoke-exposed dams. A positive correlation was observed between serum EGF levels and neutrophil numbers in bronchoalveolar lavage fluid of the dams. Interestingly, IL-17A, MCP-1, IL-22, IL13, and IFN-γ levels in anti-CD3 stimulated spleen cell culture supernatants of male pups also showed a positive correlation with EGF serum levels. In summary, our results reveal that maternal smoke exposure predisposes dams to exacerbated airway inflammation and offspring to exacerbated immune responses and both phenomena are associated with elevated EGF concentrations.

Isolated soluble fractions from spleen cell culture supernatants induce tumor enhancement

1989 ◽  
Vol 41 (2) ◽  
pp. 134-138 ◽  
Author(s):  
Ana María Eiján ◽  
Mariaa Adela Jasnis ◽  
Alicia Beatriz Motta ◽  
Sara Oisgold-Dagá
Keyword(s):  
Cell Culture ◽  
Spleen Cell ◽  
Spleen Cell Culture ◽  
Culture Supernatants ◽  
Soluble Fractions

Inhibition of mouse spleen cell activity by organotin compounds: Effect of attachment of a maltose residue to the organotin group

1993 ◽  
Vol 15 (3) ◽  
pp. 287-291 ◽  
Author(s):  
Lamya Al-Imara ◽  
Myer R. Salaman ◽  
Vojin S. Sljivic ◽  
Robert C. Poller
Keyword(s):  
Spleen Cell ◽  
Cell Activity ◽  
Organotin Compounds ◽  
Mouse Spleen ◽  
Mouse Spleen Cell

Erratum: Immunization of Normal Mouse Spleen Cell Suspensions in Vitro

Science ◽  
1966 ◽  
Vol 154 (3755) ◽  
pp. 1431-1431
Keyword(s):  
Spleen Cell ◽  
Normal Mouse ◽  
Cell Suspensions ◽  
Mouse Spleen ◽  
Mouse Spleen Cell
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