Flow cytometric DNA ploidy and S-phase fraction correlate with histopathologic indicators of tumor behavior in colorectal carcinoma

1997 ◽  
Vol 40 (4) ◽  
pp. 411-419 ◽  
Author(s):  
António E. Pinto ◽  
Paula Chaves ◽  
Paulo Fidalgo ◽  
António G. Oliveira ◽  
Carlos N. Leitøo ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

scholarly journals Flow cytometric analysis of DNA ploidy and S-phase fraction from prostatic carcinomas: implications for prognosis and response to endocrine therapy

1991 ◽  
Vol 64 (3) ◽  
pp. 578-582 ◽  
Author(s):  
T Visakorpi ◽  
O-P Kallioniemi ◽  
IYI Paronen ◽  
JJ Isola ◽  
AI Heikkinen ◽  
...  
Keyword(s):  
Endocrine Therapy ◽  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

scholarly journals Flow Cytometric Analysis of DNA Index and S-phase Fraction in the Colonic Mucosa Adjacent to Colorectal Carcinoma

1991 ◽  
Vol 24 (9) ◽  
pp. 2486-2486
Author(s):  
Kazuhiko Yoshida ◽  
Akihiro Tanabe ◽  
Tatsuro Nakagawa ◽  
Hideyo Ishida ◽  
Toshiyuki Yoda ◽  
...  
Keyword(s):  
Colorectal Carcinoma ◽  
Colonic Mucosa ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Index ◽  
Flow Cytometric

DNA ploidy and S-phase fraction in medullary carcinoma of the breast -a flow cytometric analysis using archival material

1994 ◽  
Vol 29 (3) ◽  
pp. 297-306 ◽  
Author(s):  
Lise Pedersen ◽  
J�rgen K. Larsen ◽  
Ib Jarle Christensen ◽  
Anne Lykkesfeldt ◽  
Susanne Holck ◽  
...  
Keyword(s):  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
Medullary Carcinoma ◽  
S Phase ◽  
Phase Fraction ◽  
Carcinoma Of The Breast ◽  
Archival Material ◽  
Flow Cytometric

scholarly journals Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: II. Cervical and Endometrial Cancer

2002 ◽  
Vol 24 (4-5) ◽  
pp. 147-158 ◽  
Author(s):  
Pauline Wimberger ◽  
Peter Hillemanns ◽  
Thomas Kapsner ◽  
Hermann Hepp ◽  
Rainer Kimmig
Keyword(s):  
Cervical Cancer ◽  
Endometrial Cancer ◽  
Prognostic Factors ◽  
Endometrial Carcinoma ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Dna Aneuploidy ◽  
Flow Cytometric

In gynecologic oncology valid prognostic factors are necessary to define biologically similar subgroups for analysis of therapeutic efficacy. This study is the first published prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in cervical and endometrial cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 91 specimens of cervical cancer and 73 samples of endometrial cancer. In cervical cancer neither DNA‐ploidy nor S‐phase fraction were relevant prognostic parameters. But CV of the G0G1‐peak showed prognostic relevance in cervical cancer cells, even in multivariate analysis. This interesting observation, however, seems to have no therapeutic consequence due to the small discrimination capacity of CV. In endometrial carcinoma, gross DNA‐aneuploidy (DNA‐index > 1.3) and a high percentage of proliferating cells (>75th percentile) were univariate and multivariate highly significant prognostic factors for recurrence‐free survival. Especially DNA‐aneuploidy (DI>1.3) is one of the most important independent molecular biological prognostic factors. While diagnostic curettage we could identify risk patients even preoperatively by determination of the prognostic factors like histologic tumor type, grading, cervical involvement and DNA‐ploidy. Thereby these patients could be treated primarily in an oncologic center. In conclusion, our investigations showed that the determination of DNA‐ploidy should be done in endometrial carcinoma. In cervical cancer no clinical significance for determination of DNA‐parameters was found.

No Prognostic Impact of Flow-Cytometric Measured DNA Ploidy and S-Phase Fraction in Cancer of the Uterine Cervix: A Prospective Study of 465 Patients

1995 ◽  
Vol 57 (1) ◽  
pp. 79-85 ◽  
Author(s):  
G.B. Kristensen ◽  
J. Kaern ◽  
V.M. Abeler ◽  
B. Hagmar ◽  
C.G. Tropié ◽  
...  
Keyword(s):  
Prospective Study ◽  
Uterine Cervix ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Prognostic Impact ◽  
Flow Cytometric ◽  
A Prospective Study

Flow Cytometric Analysis of DNA Ploidy and S-Phase Fraction in Primary Localized Myxofibrosarcoma: Correlations with Clinicopathological Factors, Skp2 Expression, and Patient Survival

2008 ◽  
Vol 15 (8) ◽  
pp. 2239-2249 ◽  
Author(s):  
Hsuan-Ying Huang ◽  
Wen-Wei Huang ◽  
Jing-Mei Wu ◽  
Chiung-Kuei Huang ◽  
Jun-Wen Wang ◽  
...  
Keyword(s):  
Patient Survival ◽  
Dna Ploidy ◽  
Flow Cytometric Analysis ◽  
S Phase ◽  
Phase Fraction ◽  
Clinicopathological Factors ◽  
Skp2 Expression ◽  
Flow Cytometric

scholarly journals Optimizing flow cytometric DNA ploidy and S-phase fraction as independent prognostic markers for node-negative breast cancer specimens

Cytometry ◽  
2001 ◽  
Vol 46 (3) ◽  
pp. 121-135 ◽  
Author(s):  
C.B. Bagwell ◽  
G.M. Clark ◽  
F. Spyratos ◽  
A. Chassevent ◽  
P.-O. Bendahl ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Prognostic Markers ◽  
Dna Ploidy ◽  
S Phase ◽  
Phase Fraction ◽  
Node Negative ◽  
Flow Cytometric ◽  
Node Negative Breast Cancer

scholarly journals Evaluation of Prognostic Factors Following Flow-Cytometric DNA Analysis after Cytokeratin Labelling: I. Breast Cancer

2002 ◽  
Vol 24 (4-5) ◽  
pp. 135-145 ◽  
Author(s):  
Pauline Wimberger ◽  
Peter Hillemanns ◽  
Thomas Kapsner ◽  
Hermann Hepp ◽  
Rainer Kimmig
Keyword(s):  
Breast Cancer ◽  
Prognostic Factors ◽  
Tumor Cells ◽  
Dna Ploidy ◽  
Gynecologic Oncology ◽  
Univariate Analysis ◽  
Prognostic Significance ◽  
S Phase ◽  
Phase Fraction ◽  
Flow Cytometric

In gynecologic oncology valid prognostic factors are necessary to estimate the course of disease and to define biologically similar subgroups for analysis of therapeutic efficacy. The presented study is a prospective study concerning prognostic significance of DNA ploidy and S‐phase fraction in breast cancer following enrichment of tumor cells by cytokeratin labelling. Epithelial cells were labeled by FITC‐conjugated cytokeratin antibody (CK 5, 6, 8, and CK 17) prior to flow cytometric cell cycle analysis in 327 fresh specimens of primary breast cancer. Univariate analysis in breast cancer detected the prognostic significance of DNA‐ploidy, S‐phase fraction and CV (coefficient of variation) of G0G1‐peak of tumor cells for clinical outcome, especially for nodal‐negative patients. Multivariate analysis could not confirm prognostic evidence of DNA‐ploidy and S‐phase fraction. In conclusion, in breast cancer no clinical significance for determination of DNA‐parameters was found.

A comparison between flow cytometric assessment of S-phase fraction and Nottingham histologic grade as prognostic instruments in breast cancer

2000 ◽  
Vol 63 (1) ◽  
pp. 11-15 ◽  
Author(s):  
Marie Sundquist ◽  
Sten Thorstenson ◽  
Lars Brudin ◽  
Olle Stål ◽  
Bo Nordenskjöld
Keyword(s):  
Breast Cancer ◽  
S Phase ◽  
Histologic Grade ◽  
Phase Fraction ◽  
Flow Cytometric
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