Elimination of bacterial ring rot (Corynebacterium sepedonicum [Spieck. and Kotth.] Skapt. and Burkh.) by in vitro culture of sprout tissue

Forty-seven chemicals, including antibiotics, detergents, quaternary ammonium salts, mercury compounds, disinfectants and protectants, were tested for their effectiveness as potato seed piece disinfectants after inoculating each potato set with bacterial ring rot. None of the substances tested was as effective as a solution of acidified mercuric chloride (2:1000 + 1 per cent HCl) in a 10-minute dip treatment which gave 93 to 98 per cent control. A more effective control of the disease (100 per cent) was obtained when seed stock contained only 1 per cent diseased tubers.

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LABORATORY DETECTION OF CORYNEBACTERIUM SEPEDONICUM, CAUSAL AGENT OF BACTERIAL RING ROT OF POTATOES

Canadian Journal of Botany ◽

10.1139/b56-006 ◽

1956 ◽

Vol 34 (1) ◽

pp. 48-53 ◽

Cited By ~ 10

Author(s):

H. Katznelson ◽

M. D. Sutton

Keyword(s):

Plant Extracts ◽

Causal Agent ◽

Direct Examination ◽

Bacterial Ring Rot ◽

Serological Method ◽

Corynebacterium Sepedonicum ◽

Ring Rot ◽

Incorrect Diagnosis ◽

Typical Strain ◽

Infected Material

Studies are described on the laboratory detection of bacterial ring rot of potatoes caused by Corynebacterium sepedonicum (Spieckermann and Kotthoff) Skaptason and Burkholder. A cultural–microscopic procedure, involving the preincubation of aseptically removed infected material on a suitable medium, was found in general to be more reliable than direct examination of Gram-stained smears from diseased material. This was especially noticeable with lightly infected potatoes. The use of both methods would likely decrease the possibility of incorrect diagnosis. A serological method also was found to be of value in detecting ring rot in tubers and in plant extracts. Antiserum for one typical strain of C. sepedonicum (CS850) agglutinated all strains tested, and also reacted with all infected samples examined but not with uninfected material. This organism might be used to prepare bulk antiserum for distribution to various laboratories engaged in the diagnosis of bacterial ring rot. Attempts to isolate a phage for C. sepedonicum were unsuccessful.

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ENDOPHYTIC BACTERIAL FLORA IN SOLANUM TUBEROSUM AND ITS SIGNIFICANCE IN BACTERIAL RING ROT DIAGNOSIS

Canadian Journal of Plant Science ◽

10.4141/cjps74-019 ◽

1974 ◽

Vol 54 (1) ◽

pp. 115-122 ◽

Cited By ~ 51

Author(s):

S. H. DE BOER ◽

R. J. COPEMAN

Keyword(s):

Solanum Tuberosum ◽

Bacterial Population ◽

Bacterial Flora ◽

Stem Tissue ◽

Bacterial Ring Rot ◽

Bacterial Populations ◽

Corynebacterium Sepedonicum ◽

Potato Plants ◽

Ring Rot ◽

Significant Difference

The aerobic endophytic bacterial population in "healthy" potato plants was found to vary from fewer than 1 × 103 to 4.2 × 107 cells/cm3 of stem tissue and from 0 to 1.6 × 104 cells/cm3 in tubers. No significant correlation was found between the bacterial population in stems and tubers of the same plant, nor was there a significant difference in the bacterial populations of virus-free plants when compared with PVX-infected plants. Strains of Micrococcus, Pseudomonas, Bacillus, Flavobacterium, Xanthomonas, Agrobacterium, and coryneforms were isolated in addition to several isolates that could not be identified. All isolates were nonpathogenic, including the coryneforms, some of which were morphologically indistinguishable from Corynebacterium sepedonicum but which were biochemically different.

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Specific Detection of Corynebacterium Sepedonicum in Potato with Monoclonal Antibodies as an Aid in Bacterial Ring Rot Control

Plant Pathogenic Bacteria - Current Plant Science and Biotechnology in Agriculture ◽

10.1007/978-94-009-3555-6_196 ◽

1987 ◽

pp. 906-913 ◽

Cited By ~ 1

Author(s):

S. H. De Boer

Keyword(s):

Monoclonal Antibodies ◽

Specific Detection ◽

Bacterial Ring Rot ◽

Corynebacterium Sepedonicum ◽

Ring Rot

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Characterization of a plasmid in isolates of Corynebacterium sepedonicum

Canadian Journal of Microbiology ◽

10.1139/m86-116 ◽

1986 ◽

Vol 32 (8) ◽

pp. 617-622 ◽

Cited By ~ 6

Author(s):

Mervyn C. Clark ◽

C. Harold Lawrence

Keyword(s):

Sodium Dodecyl ◽

Positive Bacterium ◽

Bacterial Ring Rot ◽

Hybridization Probe ◽

Corynebacterium Sepedonicum ◽

Agarose Gels ◽

Cell Cleavage ◽

Ring Rot ◽

Single Plasmid

Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 °C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight estimated to be 31 megadaltons (46 kilobases), as determined from its relative mobility in agarose gels, from its contour dimensions in electron micrographs, and from the size of the fragments generated when it was cleaved with various restriction endonucleases. Thirteen widely divergent isolates of C. sepedonicum were screened for the presence of plasmid, and of these, 11 were shown to harbour a single plasmid at a level of about 30 copies per cell. Cleavage of the plasmid with PstI gave an identical banding pattern in agarose gels for the fragments from all of the plasmid-carrying isolates. The relevance of plasmid incidence and distribution in C. sepedonicum is discussed in relation to the possibility of developing a test for the detection of bacterial ring rot by using plasmid DNA as a hybridization probe.

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CHANGE OF CONCENTRATION OF HYDROGEN PEROXIDE AND cAMP IN TRANSGENIC PLANTS IN VITRO GRADE SCARB DEPENDING ON RESISTANCE TO BACTERIAL RING ROT PATHOGEN

Book of proceedings of the All-Russian Scientific Conference with International Participation and Schools of Young Scientists "Mechanisms of resistance of plants and microorganisms to unfavorable environmental" (parts I, II) ◽

10.31255/978-5-94797-319-8-786-789 ◽

2018 ◽

Author(s):

N.V. Filinova ◽

L.A. Lomovatskaya ◽

A.S. Romanenko

Keyword(s):

Hydrogen Peroxide ◽

Transgenic Plants ◽

Bacterial Ring Rot ◽

Ring Rot

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Categorisation of reactions and enumeration of bacteria in potato cultivars inoculated with the causal agent of bacterial ring rot

Plant Protection Science ◽

10.17221/44/2018-pps ◽

2018 ◽

Vol 55 (No. 1) ◽

pp. 11-22

Author(s):

Pánková Iveta ◽

Krejzar Václav ◽

Krejzarová Radka

Keyword(s):

Inoculum Size ◽

Potato Cultivars ◽

Clavibacter Michiganensis ◽

Bacterial Ring Rot ◽

Potato Plants ◽

Clavibacter Michiganensis Subsp ◽

Ring Rot ◽

Foliage Symptom ◽

Symptom Group

Variability in the responses of plants propagated from in vitro tissue cultures of 52 ware and industrial potato cultivars to different Clavibacter michiganensis subsp. sepedonicus inoculum size was tested during 2015–2017. Bacterial ring rot symptoms on plants and tubers xylem vessels were recorded for 8 weeks and the susceptibility index (SI) for individual cultivars was calculated. Based on foliage symptoms, potato cultivars were placed into three symptoms groups. The symptomless group had SIs ≤ 1, for the moderate symptom group SIs ranged from 1.01 to 2.99, and the severe symptom group had SIs ≥ 3.0. The pathogen concentrations in vascular vessels of all infected potato plants increased during the experiment regardless of the foliage symptom group.

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