LABORATORY DETECTION OF CORYNEBACTERIUM SEPEDONICUM, CAUSAL AGENT OF BACTERIAL RING ROT OF POTATOES

1956 ◽  
Vol 34 (1) ◽  
pp. 48-53 ◽  
Author(s):  
H. Katznelson ◽  
M. D. Sutton
Keyword(s):  
Plant Extracts ◽  
Causal Agent ◽  
Direct Examination ◽  
Bacterial Ring Rot ◽  
Serological Method ◽  
Corynebacterium Sepedonicum ◽  
Ring Rot ◽  
Incorrect Diagnosis ◽  
Typical Strain ◽  
Infected Material

Studies are described on the laboratory detection of bacterial ring rot of potatoes caused by Corynebacterium sepedonicum (Spieckermann and Kotthoff) Skaptason and Burkholder. A cultural–microscopic procedure, involving the preincubation of aseptically removed infected material on a suitable medium, was found in general to be more reliable than direct examination of Gram-stained smears from diseased material. This was especially noticeable with lightly infected potatoes. The use of both methods would likely decrease the possibility of incorrect diagnosis. A serological method also was found to be of value in detecting ring rot in tubers and in plant extracts. Antiserum for one typical strain of C. sepedonicum (CS850) agglutinated all strains tested, and also reacted with all infected samples examined but not with uninfected material. This organism might be used to prepare bulk antiserum for distribution to various laboratories engaged in the diagnosis of bacterial ring rot. Attempts to isolate a phage for C. sepedonicum were unsuccessful.

ESSAI DE DIFFERENTS PRODUITS CHIMIQUES COMME DESINFECTANTS DES ECLATS (GERMES) DE POMMES DE TERRE INOCULES AVEC CORYNEBACTERIUM SEPEDONICUM

1960 ◽  
Vol 40 (2) ◽  
pp. 383-387
Author(s):  
R. Paquin ◽  
J. Santerre ◽  
H. Généreux ◽  
L. J. Coulombe
Keyword(s):  
Mercuric Chloride ◽  
Quaternary Ammonium Salts ◽  
Ammonium Salts ◽  
Effective Control ◽  
Potato Seed ◽  
Bacterial Ring Rot ◽  
Corynebacterium Sepedonicum ◽  
Mercury Compounds ◽  
Ring Rot ◽  
Potato Seed Piece

Forty-seven chemicals, including antibiotics, detergents, quaternary ammonium salts, mercury compounds, disinfectants and protectants, were tested for their effectiveness as potato seed piece disinfectants after inoculating each potato set with bacterial ring rot. None of the substances tested was as effective as a solution of acidified mercuric chloride (2:1000 + 1 per cent HCl) in a 10-minute dip treatment which gave 93 to 98 per cent control. A more effective control of the disease (100 per cent) was obtained when seed stock contained only 1 per cent diseased tubers.

ENDOPHYTIC BACTERIAL FLORA IN SOLANUM TUBEROSUM AND ITS SIGNIFICANCE IN BACTERIAL RING ROT DIAGNOSIS

1974 ◽  
Vol 54 (1) ◽  
pp. 115-122 ◽  
Author(s):  
S. H. DE BOER ◽  
R. J. COPEMAN
Keyword(s):  
Solanum Tuberosum ◽  
Bacterial Population ◽  
Bacterial Flora ◽  
Stem Tissue ◽  
Bacterial Ring Rot ◽  
Bacterial Populations ◽  
Corynebacterium Sepedonicum ◽  
Potato Plants ◽  
Ring Rot ◽  
Significant Difference

The aerobic endophytic bacterial population in "healthy" potato plants was found to vary from fewer than 1 × 103 to 4.2 × 107 cells/cm3 of stem tissue and from 0 to 1.6 × 104 cells/cm3 in tubers. No significant correlation was found between the bacterial population in stems and tubers of the same plant, nor was there a significant difference in the bacterial populations of virus-free plants when compared with PVX-infected plants. Strains of Micrococcus, Pseudomonas, Bacillus, Flavobacterium, Xanthomonas, Agrobacterium, and coryneforms were isolated in addition to several isolates that could not be identified. All isolates were nonpathogenic, including the coryneforms, some of which were morphologically indistinguishable from Corynebacterium sepedonicum but which were biochemically different.

Étude du rôle des sucres dans la résistance des pommes de terre au flétrissement bactérien

1969 ◽  
Vol 15 (8) ◽  
pp. 907-916 ◽  
Author(s):  
Roger Paquin ◽  
Guy Pelletier
Keyword(s):  
Storage Temperature ◽  
Sugar Content ◽  
Rapid Decrease ◽  
Causal Agent ◽  
Potato Tubers ◽  
Varietal Resistance ◽  
Corynebacterium Sepedonicum ◽  
Ring Rot ◽  
Potato Resistance

Potato resistance to ring rot does not seem to be related to sugars present in stems and tubers in opposition to the conclusions of Rozhalin. Sugars contained in those organs exceed the amount required by the causal agent, Corynebacterium sepedonicum (Spieck. & Kott.) Skapt. and Burkh., for its growth. Moreover, no correlation could be shown between sugar content and varietal resistance. Sugars added to potato extracts did not influence the growth of C. sepedonicum on these extracts. Moreover, a rise of the storage temperature of potato tubers which caused a rapid decrease in their sugar content did not influence the growth of C. sepedonicum on extracts of the same tubers.

Characterization of a plasmid in isolates of Corynebacterium sepedonicum

1986 ◽  
Vol 32 (8) ◽  
pp. 617-622 ◽  
Author(s):  
Mervyn C. Clark ◽  
C. Harold Lawrence
Keyword(s):  
Sodium Dodecyl ◽  
Positive Bacterium ◽  
Bacterial Ring Rot ◽  
Hybridization Probe ◽  
Corynebacterium Sepedonicum ◽  
Agarose Gels ◽  
Cell Cleavage ◽  
Ring Rot ◽  
Single Plasmid

Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 °C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight estimated to be 31 megadaltons (46 kilobases), as determined from its relative mobility in agarose gels, from its contour dimensions in electron micrographs, and from the size of the fragments generated when it was cleaved with various restriction endonucleases. Thirteen widely divergent isolates of C. sepedonicum were screened for the presence of plasmid, and of these, 11 were shown to harbour a single plasmid at a level of about 30 copies per cell. Cleavage of the plasmid with PstI gave an identical banding pattern in agarose gels for the fragments from all of the plasmid-carrying isolates. The relevance of plasmid incidence and distribution in C. sepedonicum is discussed in relation to the possibility of developing a test for the detection of bacterial ring rot by using plasmid DNA as a hybridization probe.

scholarly journals Antimicrobial efficacy of some plant extracts on bacterial ring rot pathogen, clavibacter michiganensis ssp. sepedonicus

2017 ◽  
Vol 1 (1) ◽  
pp. 85-88 ◽  
Author(s):  
Elena Ichim ◽  
Luminita Marutescu ◽  
Marcela Popa ◽  
Stelica Cristea
Keyword(s):  
Plant Extracts ◽  
Plant Disease Resistance ◽  
Plant Pathogens ◽  
Natural Compounds ◽  
Diffusion Method ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Alternative Strategies ◽  
Natural Substances ◽  
Ring Rot

Abstract Control of plant bacterial diseases remains difficult due to the limited availability of efficient plant protection products with reduced negative effects either in the environment or with human and animal health. In order to reduce the usage of chemical pesticides alternative strategies for controlling plant pathogens and improve plant disease resistance are promoted. The aim of the study was to investigate the antibacterial activity of some natural compounds (plant extracts of Tamarix ramosissima, Rosmarinus officinalis, Chelidonium majus, Silybum marianum, Satureja hortensis essential oil and propolis) against bacterial ring rot pathogen, Clavibacter michiganensis ssp. sepedonicus (Cms). An agar diffusion method was used for the screening of the inhibitory effect of natural compounds on bacterial strains’ growth. Minimum inhibition concentrations (MICs) were determined by a twofold serial dilution method. The anti-pathogenic activity was investigated by the study of anti-biofilm activity of natural substances. The analyzed natural substances showed a good microbicidal activity and anti-biofilm activity. The results obtained from this study may contribute to the development of new bio-control agents as alternative strategies for prevention and control of ring rot pathogen.

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