Specific Detection of Corynebacterium Sepedonicum in Potato with Monoclonal Antibodies as an Aid in Bacterial Ring Rot Control

1987 ◽  
pp. 906-913 ◽  
Author(s):  
S. H. De Boer
Keyword(s):  
Monoclonal Antibodies ◽  
Specific Detection ◽  
Bacterial Ring Rot ◽  
Corynebacterium Sepedonicum ◽  
Ring Rot

ESSAI DE DIFFERENTS PRODUITS CHIMIQUES COMME DESINFECTANTS DES ECLATS (GERMES) DE POMMES DE TERRE INOCULES AVEC CORYNEBACTERIUM SEPEDONICUM

1960 ◽  
Vol 40 (2) ◽  
pp. 383-387
Author(s):  
R. Paquin ◽  
J. Santerre ◽  
H. Généreux ◽  
L. J. Coulombe
Keyword(s):  
Mercuric Chloride ◽  
Quaternary Ammonium Salts ◽  
Ammonium Salts ◽  
Effective Control ◽  
Potato Seed ◽  
Bacterial Ring Rot ◽  
Corynebacterium Sepedonicum ◽  
Mercury Compounds ◽  
Ring Rot ◽  
Potato Seed Piece

Forty-seven chemicals, including antibiotics, detergents, quaternary ammonium salts, mercury compounds, disinfectants and protectants, were tested for their effectiveness as potato seed piece disinfectants after inoculating each potato set with bacterial ring rot. None of the substances tested was as effective as a solution of acidified mercuric chloride (2:1000 + 1 per cent HCl) in a 10-minute dip treatment which gave 93 to 98 per cent control. A more effective control of the disease (100 per cent) was obtained when seed stock contained only 1 per cent diseased tubers.

LABORATORY DETECTION OF CORYNEBACTERIUM SEPEDONICUM, CAUSAL AGENT OF BACTERIAL RING ROT OF POTATOES

1956 ◽  
Vol 34 (1) ◽  
pp. 48-53 ◽  
Author(s):  
H. Katznelson ◽  
M. D. Sutton
Keyword(s):  
Plant Extracts ◽  
Causal Agent ◽  
Direct Examination ◽  
Bacterial Ring Rot ◽  
Serological Method ◽  
Corynebacterium Sepedonicum ◽  
Ring Rot ◽  
Incorrect Diagnosis ◽  
Typical Strain ◽  
Infected Material

Studies are described on the laboratory detection of bacterial ring rot of potatoes caused by Corynebacterium sepedonicum (Spieckermann and Kotthoff) Skaptason and Burkholder. A cultural–microscopic procedure, involving the preincubation of aseptically removed infected material on a suitable medium, was found in general to be more reliable than direct examination of Gram-stained smears from diseased material. This was especially noticeable with lightly infected potatoes. The use of both methods would likely decrease the possibility of incorrect diagnosis. A serological method also was found to be of value in detecting ring rot in tubers and in plant extracts. Antiserum for one typical strain of C. sepedonicum (CS850) agglutinated all strains tested, and also reacted with all infected samples examined but not with uninfected material. This organism might be used to prepare bulk antiserum for distribution to various laboratories engaged in the diagnosis of bacterial ring rot. Attempts to isolate a phage for C. sepedonicum were unsuccessful.

ENDOPHYTIC BACTERIAL FLORA IN SOLANUM TUBEROSUM AND ITS SIGNIFICANCE IN BACTERIAL RING ROT DIAGNOSIS

1974 ◽  
Vol 54 (1) ◽  
pp. 115-122 ◽  
Author(s):  
S. H. DE BOER ◽  
R. J. COPEMAN
Keyword(s):  
Solanum Tuberosum ◽  
Bacterial Population ◽  
Bacterial Flora ◽  
Stem Tissue ◽  
Bacterial Ring Rot ◽  
Bacterial Populations ◽  
Corynebacterium Sepedonicum ◽  
Potato Plants ◽  
Ring Rot ◽  
Significant Difference

The aerobic endophytic bacterial population in "healthy" potato plants was found to vary from fewer than 1 × 103 to 4.2 × 107 cells/cm3 of stem tissue and from 0 to 1.6 × 104 cells/cm3 in tubers. No significant correlation was found between the bacterial population in stems and tubers of the same plant, nor was there a significant difference in the bacterial populations of virus-free plants when compared with PVX-infected plants. Strains of Micrococcus, Pseudomonas, Bacillus, Flavobacterium, Xanthomonas, Agrobacterium, and coryneforms were isolated in addition to several isolates that could not be identified. All isolates were nonpathogenic, including the coryneforms, some of which were morphologically indistinguishable from Corynebacterium sepedonicum but which were biochemically different.

Characterization of a plasmid in isolates of Corynebacterium sepedonicum

1986 ◽  
Vol 32 (8) ◽  
pp. 617-622 ◽  
Author(s):  
Mervyn C. Clark ◽  
C. Harold Lawrence
Keyword(s):  
Sodium Dodecyl ◽  
Positive Bacterium ◽  
Bacterial Ring Rot ◽  
Hybridization Probe ◽  
Corynebacterium Sepedonicum ◽  
Agarose Gels ◽  
Cell Cleavage ◽  
Ring Rot ◽  
Single Plasmid

Lysis of mid-log phase cells of the Gram-positive bacterium, Corynebacterium sepedonicum, by a combination of lysozyme treatment and incubation with alkaline sodium dodecyl sulfate at 56 °C led to the recovery of a single plasmid. The plasmid was purified in CsCl density gradients, and its molecular weight estimated to be 31 megadaltons (46 kilobases), as determined from its relative mobility in agarose gels, from its contour dimensions in electron micrographs, and from the size of the fragments generated when it was cleaved with various restriction endonucleases. Thirteen widely divergent isolates of C. sepedonicum were screened for the presence of plasmid, and of these, 11 were shown to harbour a single plasmid at a level of about 30 copies per cell. Cleavage of the plasmid with PstI gave an identical banding pattern in agarose gels for the fragments from all of the plasmid-carrying isolates. The relevance of plasmid incidence and distribution in C. sepedonicum is discussed in relation to the possibility of developing a test for the detection of bacterial ring rot by using plasmid DNA as a hybridization probe.

Insect transmission of the bacterial ring rot pathogen

1991 ◽  
Vol 68 (6) ◽  
pp. 363-372 ◽  
Author(s):  
R. D. Christie ◽  
A. C. Sumalde ◽  
J. T. Schulz ◽  
N. C. Gudmestad
Keyword(s):  
Bacterial Ring Rot ◽  
Ring Rot ◽  
Insect Transmission

scholarly journals Teknik Inokulasi Buatan Clavibacter michiganensis subsp. sepedonicus, Penyebab Penyakit Busuk Cincin Bakteri, pada Tanaman Kentang (Solanum tuberosum L.)

Agrikultura ◽  
2016 ◽  
Vol 27 (2) ◽  
Author(s):  
Luciana Djaya ◽  
Ineu Sulastrini ◽  
Iin Rusita
Keyword(s):  
Solanum Tuberosum ◽  
Solanum Tuberosum L ◽  
Block Design ◽  
Clavibacter Michiganensis ◽  
Bacterial Ring Rot ◽  
Seed Tubers ◽  
Clavibacter Michiganensis Subsp ◽  
Ring Rot ◽  
Inoculation Techniques ◽  
Rot Disease

ABSTRACT Inoculation Techniques of Clavibacter michiganensis subsp. sepedonicus, the Cause of Bacterial Ring Rot Disease, on Potato (Solanum tuberosum L.). Clavibacter michiganensis subsp. sepedonicus, the cause of bacterial ring rot disease on potatoes, has been detected in potato fields in Pangalengan. To anticipate the spread of the pathogen, researches on the desease epidemiology are urgent to be carried out. Artificial inoculation techniques will be useful in the epidemiological studies. The objective of this reasearch was to evaluate some inoculation techniques, which are simple, cheap and fast in causing disease symptoms. The experiment was carried out at the laboratory and glasshouse of Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. The experiment was arranged in the randomized block design with five treatments of inoculation technique and five replications. The treatments were (a) soaking wounded seed tubers in pathogen suspension, (b) soaking not wounded seed tubers in pathogen suspension, (c) pathogen suspension was injected into leaf axil, (d) pathogen suspension was injected into seed tubers, and (e) pathogen suspension was poured into the planting holes. The results showed that stabbing and soaking tubers in pathogen suspension caused the shortest incubation period (17 days after inoculation) and the highest disease incidence (60%). Keywords : Clavibacter michiganensis subsp. sepedonicus, Bacterial ring rot, Potatoes, Inoculation techniquesABSTRAKClavibacter michiganensis subsp. sepedonicus (Cms), penyebab penyakit busuk cincin bakteri pada tanaman kentang, telah terdeteksi keberadaannya pada pertanaman kentang di Pangalengan. Dalam upaya mencegah penyebaran penyakit busuk cincin bakteri di Indonesia, perlu adanya studi epidemiologi patogen tersebut. Pada penelitian epidemiologi akan diperlukan cara menginokulasi tanaman secara buatan. Tujuan dari penelitian ini adalah untuk mendapatkan teknik inokulasi buatan yang dapat menyebabkan periode inkubasi yang lebih singkat dan persentase kejadian penyakit busuk cincin bakteri paling tinggi pada tanaman kentang. Percobaan dilaksanakan di rumah kaca dan laboratorium penyakit Balai Penelitian Tanaman Sayuran (Balitsa), Lembang. Percobaan dirancang secara rancangan acak kelompok dengan lima perlakuan teknik inokulasi Cms dan lima ulangan, yaitu inokulasi dengan (a) merendam ubi benih yang telah dilukai dalam suspensi bakteri, (b) merendam ubi benih tanpa dilukai dalam suspensi bakteri, (c) suspensi bakteri ditusukkan pada ketiak daun tanaman kentang, (d) suspensi bakteri ditusukkan pada ubi benih, dan (e) suspensi bakteri disiramkan pada lubang tanam. Masing-masing ulangan terdiri dari lima tanaman. Hasil penelitian menunjukkan bahwa dari lima teknik inokulasi buatan yang dilakukan, inokulasi dengan melukai ubi benih dan merendamnya dalam suspensi patogen menghasilkanJurnal Agrikultura 2016, 27 (2): 66-71ISSN 0853-2885Teknik Inokulasi Buatan Clavibacter….67periode inkubasi tersingkat, yaitu 17 hari setelah inokulasi, dan persentase kejadian penyakit tertinggi yaitu sebesar 60%.Kata Kunci : Clavibacter michiganensis subsp. sepedonicus, Busuk cincin bakteri, Kentang, Inokulasi buatan

Symptom Expression of Mainstream and Specialty Potato Cultivars to Bacterial Ring Rot (Clavibacter sepedonicus) and Evaluation of in-Field Detection

2019 ◽  
Vol 96 (4) ◽  
pp. 427-444 ◽  
Author(s):  
Jonathan L. Whitworth ◽  
Rachel A. Selstedt ◽  
Alan A. G. Westra ◽  
Phil Nolte ◽  
Kasia Duellman ◽  
...  
Keyword(s):  
Potato Cultivars ◽  
Symptom Expression ◽  
Bacterial Ring Rot ◽  
Field Detection ◽  
Ring Rot
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