Effects of vitamin D metabolites and analogs on bone collagen synthesis in vitro

1982 ◽  
Vol 34 (1) ◽  
Author(s):  
F. Richard Bringhurst ◽  
John T. Potts
Keyword(s):  
Vitamin D ◽  
Collagen Synthesis ◽  
Bone Collagen ◽  
Vitamin D Metabolites ◽  
Bone Collagen Synthesis

scholarly journals Vitamin A and bone formation. Effect of an excess of retinol on bone collagen synthesis in vitro

1985 ◽  
Vol 226 (3) ◽  
pp. 789-795 ◽  
Author(s):  
I Dickson ◽  
J Walls
Keyword(s):  
Bone Formation ◽  
Collagen Synthesis ◽  
Bone Collagen ◽  
Dependent Manner ◽  
Stimulate Bone Resorption ◽  
Dna And Rna ◽  
A Cell ◽  
Dose Dependent ◽  
Bone Collagen Synthesis

The influence of an excess of retinol on bone formation was studied by using cultures of embryonic-chick calvaria. Retinol decreased collagen synthesis in a dose-dependent manner, non-collagenous protein synthesis being relatively unaffected. Collagen synthesis was significantly inhibited after 24 h of culture with retinol and was progressively decreased, compared with control cultures containing no retinol, as the period of culture was increased. The effect of retinol on collagen synthesis could be reversed by incubation of calvaria for further periods in retinol-free medium. Incorporation of [3H]thymidine and [3H]uridine into DNA and RNA respectively was not altered by culturing calvaria with retinol for 22 h. These latter findings, and the selectivity for collagen synthesis, all suggested that the effect observed was not a cell-toxicity phenomenon. The effect of retinol on collagen synthesis by chick calvarial osteoblasts was probably direct and not mediated by osteoclasts, since a negligible number of the latter cells is present in chick calvaria. In cultures of neonatal murine calvaria, which contain many osteoclasts, retinol similarly inhibited synthesis of collagen, but not of non-collagenous protein; the concentrations of retinol necessary to produce the response were similar to those required to stimulate bone resorption in vitro.

Effects of Glucocorticoids on Fetal Rat Bone Collagen Synthesis in Vitro*

Endocrinology ◽  
1979 ◽  
Vol 104 (3) ◽  
pp. 715-721 ◽  
Author(s):  
JOHN W. DIETRICH ◽  
ERNESTO M. CANALIS ◽  
DONNA M. MAINA ◽  
LAWRENCE G. RAISZ
Keyword(s):  
Collagen Synthesis ◽  
Bone Collagen ◽  
Fetal Rat ◽  
Bone Collagen Synthesis ◽  
Rat Bone

Hormonal Control of Bone Collagen Synthesis in Vitro. Effects of Insulin and Glucagon*

Endocrinology ◽  
1977 ◽  
Vol 100 (3) ◽  
pp. 668-674 ◽  
Author(s):  
ERNESTO M. CANALIS ◽  
JOHN W. DIETRICH ◽  
DONNA M. MAINA ◽  
LAWRENCE G. RAISZ
Keyword(s):  
Collagen Synthesis ◽  
Hormonal Control ◽  
Bone Collagen ◽  
In Vitro Effects ◽  
Bone Collagen Synthesis

Effects of phosphate and 1,25(OH)2D3 on in vitro bone collagen synthesis in the hypophosphatemic mouse

1983 ◽  
Vol 35 (1) ◽  
pp. 383-391 ◽  
Author(s):  
Brigitte Ecarot-Charrier ◽  
Francis H. Glorieux
Keyword(s):  
Collagen Synthesis ◽  
Bone Collagen ◽  
Bone Collagen Synthesis

Effect of somatomedin and growth hormone on bone collagen synthesis in vitro

Metabolism ◽  
1977 ◽  
Vol 26 (10) ◽  
pp. 1079-1087 ◽  
Author(s):  
Ernesto M. Canalis ◽  
Raymond L. Hintz ◽  
John W. Dietrich ◽  
Donna M. Maina ◽  
Lawrence G. Raisz
Keyword(s):  
Growth Hormone ◽  
Collagen Synthesis ◽  
Bone Collagen ◽  
Bone Collagen Synthesis

scholarly journals Collagen synthesis and carbohydrate metabolism of rachitic bone

1968 ◽  
Vol 109 (1) ◽  
pp. 101-106 ◽  
Author(s):  
C. R. Paterson ◽  
P. Fourman
Keyword(s):  
Vitamin D ◽  
Vitamin D Deficiency ◽  
Collagen Synthesis ◽  
Direct Action ◽  
Bone Collagen ◽  
Salt Solutions ◽  
Normal Bone ◽  
Deficiency Rickets

1. This paper reports studies on the metabolism of bone from normal chicks and from chicks with vitamin D-deficiency rickets. Both in vitro and in vivo there was an increased incorporation of [14C]proline into collagen hydroxyproline by rachitic bone. The proportion of the collagen that was soluble in cold salt solutions was greater with the rachitic bone. These results show that in rickets there is an increased synthesis of bone collagen, but they do not provide any evidence of a defect in the maturation of collagen. 2. Rachitic bone incubated aerobically in vitro consumed more glucose and released more lactate than normal bone. Bone from rachitic chicks treated with vitamin D 48hr. previously had rates of glycolysis that were nearly normal. Though we were unable to show any direct action of vitamin D in vitro, we consider that vitamin D probably has a direct action on bone, possibly related to matrix biosynthesis.

Influence of vitamin a on bone collagen synthesis in vitro

Bone ◽  
1985 ◽  
Vol 6 (4) ◽  
pp. 277-277
Author(s):  
I. Dickson ◽  
J. Walls
Keyword(s):  
Vitamin A ◽  
Collagen Synthesis ◽  
Bone Collagen ◽  
Bone Collagen Synthesis
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