Quantitative analysis of brain synaptophysin (p38) in offspring of male rats with chronic morphine intoxication

The content of neuromediators and its metabolites in the cortex of cerebral hemispheres, in thalamus and brain stem was studied under chronic morphine intoxication (7-21 days). The morphine intake during 7-14 days was accompanied by changes of catecholamine system functioning, which was the most pronounced in the thalamus and the brain stem. These changes included increased secretion of dophamine and noradrenaline, their decrease in the brain tissue, and the increased content of their metabolites. The changes of serotonin and GABA content were less pronounced and included a decrease of serotonin level and the increase of the GABA content in different periods of narcotization.

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A sex difference in endogenous opioid regulation of the posterior pituitary response to stress in the rat

Journal of Endocrinology ◽

10.1677/joe.0.1110239 ◽

1986 ◽

Vol 111 (2) ◽

pp. 239-244 ◽

Cited By ~ 19

Author(s):

D. A. Carter ◽

T. D. M. Williams ◽

S. L. Lightman

Keyword(s):

Sex Difference ◽

Gonadal Hormones ◽

Endogenous Opioids ◽

Female Rats ◽

Male Rats ◽

Posterior Pituitary ◽

Endogenous Opioid ◽

Chronic Morphine ◽

Response To Stress ◽

Naloxone Treatment

ABSTRACT The influence of endogenous opioids on the posterior pituitary response to stress was investigated by measuring plasma hormone levels in immobilized male and female rats following either acute naloxone treatment or prolonged morphine administration. Naloxone significantly potentiated the oxytocin and arginine vasopressin (AVP) response to immobilization, but in female rats only. The responses of morphine-treated male rats showed differences compared with vehicle-treated controls, although chronic morphine treatment did not reliably alter the oxytocin or AVP responses to immobilization in males or females. In a further experiment to investigate the role of gonadal hormones in determining the sex difference in responsiveness to naloxone, it was found that acute naloxone treatment significantly potentiated the posterior pituitary response to stress in castrated male rats. These results extend previous studies showing a sex difference in stress-induced secretion of posterior pituitary hormones, providing evidence of a sexual dimorphism in the endogenous opioid regulation of this response which is partly determined by circulating gonadal hormones. J. Endocr. (1986) 111, 239–244

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Effect of Chronic Morphine Consumption on Synaptic Plasticity of Rat’s Hippocampus: A Transmission Electron Microscopy Study

Neurology Research International ◽

10.1155/2013/290414 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 8

Author(s):

Mohammad Hassan Heidari ◽

Abdollah Amini ◽

Zohreh Bahrami ◽

Ali Shahriari ◽

Abolfazle Movafag ◽

...

Keyword(s):

Synaptic Plasticity ◽

Postsynaptic Density ◽

Morphine Consumption ◽

Male Rats ◽

Ultrastructural Changes ◽

Morphine Dependence ◽

Morphine Group ◽

Chronic Morphine ◽

Ultrastructural Studies ◽

And Control

It is well known that the synapses undergo some changes in the brain during the course of normal life and under certain pathological or experimental circumstances. One of the main goals of numerous researchers has been to find the reasons for these structural changes. In the present study, we investigated the effects of chronic morphine consumption on synaptic plasticity, postsynaptic density thickness, and synaptic curvatures of hippocampus CA1 area of rats. So for reaching these goals, 24N-Mary male rats were randomly divided into three groups, morphine (n=8), placebo (n=8), and control (n=8) groups. In the morphine group, complex of morphine (0.1, 0.2, 0.3, and 0.4) mg/mL and in the placebo (sucrose) group complex of sucrose (% 0.3) were used for 21 days. After the end of drug treatment the animals were scarified and perfused intracardinally and finally the CA1 hippocampal samples were taken for ultrastructural studies, and then the obtained data were analyzed by SPSS and one-way analysis of variance. Our data indicated that synaptic numbers per nm3change significantly in morphine group compared to the other two groups (placebo and control) (P<0.001) and also statistical analysis revealed a significant difference between groups in terms of thickness of postsynaptic density (P<0.001) and synaptic curvature (P<0.007). It seems that morphine dependence in rats plays a main role in the ultrastructural changes of hippocampus.

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Neurotransmitter systems of some brain regions of rats subjected to chronic morphine intoxication

Biochemistry (Moscow) Supplement Series B Biomedical Chemistry ◽

10.1134/s1990750811020065 ◽

2011 ◽

Vol 5 (2) ◽

pp. 175-179

Author(s):

S. V. Lelevich ◽

A. A. Novokshonov

Keyword(s):

Brain Regions ◽

Chronic Morphine ◽

Neurotransmitter Systems ◽

Chronic Morphine Intoxication

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Effect of Intracerebroventricular Morphine Withdrawal on Anxiety Behavior in Male Rats Reared in Social Isolation

Quarterly of Horizon of Medical Sciences ◽

10.32598/hms.25.4.352 ◽

2019 ◽

Vol 25 (4) ◽

pp. 352-363

Author(s):

Ghasemali Khodabandeh ◽

◽

Gholamhassan Vaezi ◽

Vida ‎ Hojati ◽

Sharam ‎ Sharafi ◽

...

Keyword(s):

Social Isolation ◽

Sulfate Solution ◽

Morphine Withdrawal ◽

Morphine Sulfate ◽

Male Rats ◽

Saline Control ◽

Control Group ◽

Emotional States ◽

Daily Consumption ◽

Chronic Morphine

Aims Narcotics prescription has controversial effects on the occurrence of anxiety processes; however, its acute and chronic effects on behavioral differences in social isolation are unclear in the processes of dependence and withdrawal. The present study aimed to investigate the effects of acute and chronic intracerebroventricular morphine sulfate withdrawal on the fear and anxiety behaviors of male rats reared in social isolation. Methods & Materials The present experimental study investigated 32 male 21-day-old male weaned Wistar rats that were divided into two groups of saline (control) and morphine receivers (test). They were then divided into acute and chronic subgroups that were reared under social isolation conditions. The rats of the acute daily consumption group received 10 μg/kg of morphine sulfate solution via intracerebroventricular injection for 10 days, but the chronic rats received it for 60 days. After the end of dependence by its withdrawal, the rats were quitted for 5 days, and their anxiety levels were measured using the Elevated Plus Maze (EPM). The obtained data were analyzed in SPSS using one-way Analysis of Variance (ANOVA), Tukey’s posthoc test and Paired Samples t-test. Findings The research results indicated that the percentage of time and number of open arm entries in rats reared in social isolation significantly decreased during the dependence phase and 5 days after withdrawal in acute and chronic groups (P<0.001). Furthermore, their anxiety rate increased compared to the control group. The findings also suggested a higher incidence of anxiety among chronic consumer groups than acute consumer groups after abstinence. Conclusion The study findings indicated that the discontinuation of morphine consumption in social isolation could increase the incidence of anxiety behaviors in rats. Therefore, negative emotional states associated with acute and chronic morphine withdrawal could lead to anxiety-like behaviors.Keywords: Anxiety, Morphine, Social isolation, Rats.

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