Cell-surface charge and cell-surface hydrophobicity of collagen-bindingAeromonas andVibrio strains

1995 ◽  
Vol 164 (3) ◽  
pp. 223-230 ◽  
Author(s):  
F. Ascencio ◽  
G. Johansson ◽  
T. Wadström
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Cell Surface Charge

Cell-surface hydrophobicity and cell-surface charge of Azospirillum spp.

2006 ◽  
Vol 24 (2) ◽  
pp. 159-172 ◽  
Author(s):  
Thelma Castellanos ◽  
Felipe Ascencio ◽  
Yoav Bashan
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Cell Surface Charge

scholarly journals Effects of Hydrophobic and Electrostatic Cell Surface Properties of Bacteria on Feeding Rates of Heterotrophic Nanoflagellates

2001 ◽  
Vol 67 (2) ◽  
pp. 814-820 ◽  
Author(s):  
Carsten Matz ◽  
Klaus Jürgens
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Food Selection ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Common Species ◽  
Electrostatic Charge ◽  
Heterotrophic Nanoflagellates ◽  
Feeding Rates ◽  
Protozoan Grazing

ABSTRACT The influence of cell surface hydrophobicity and electrostatic charge of bacteria on grazing rates of three common species of interception-feeding nanoflagellates was examined. The hydrophobicity of bacteria isolated from freshwater plankton was assessed by using two different methods (bacterial adhesion to hydrocarbon and hydrophobic interaction chromatography). The electrostatic charge of the cell surface (measured as zeta potential) was analyzed by microelectrophoresis. Bacterial ingestion rates were determined by enumerating bacteria in food vacuoles by immunofluorescence labelling via strain-specific antibodies. Feeding rates varied about twofold for each flagellate species but showed no significant dependence on prey hydrophobicity or surface charge. Further evidence was provided by an experiment involving flagellate grazing on complex bacterial communities in a two-stage continuous culture system. The hydrophobicity values of bacteria that survived protozoan grazing were variable, but the bacteria did not tend to become more hydrophilic. We concluded that variability in bacterial cell hydrophobicity and variability in surface charge do not severely affect uptake rates of suspended bacteria or food selection by interception-feeding flagellates.

Cell surface and exopolymer characterization of laboratory stabilized activated sludge from a beverage bottling plant

2001 ◽  
Vol 43 (6) ◽  
pp. 175-184 ◽  
Author(s):  
S. M. Boyette ◽  
J. M. Lovett ◽  
W. G. Gaboda ◽  
J. A. Soares
Keyword(s):  
Cell Surface ◽  
Activated Sludge ◽  
Surface Charge ◽  
Electrophoretic Mobility ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Food Sources ◽  
Colloid Titration ◽  
High Concentrations

Fermentor-stabilized activated sludge from an industrial beverage bottling plant was grown on three different food sources: normal plant wastewater, plant wastewater containing high sucrose concentrations, and a synthetic glucose-based feed stock. Surface charge, hydrophobicity, and exopolysaccharide composition were measured on the stabilized bacterial flocs. Cell surface charge was measured by electrophoretic mobility, dye exchange titration, and a standard colloid titration, while cell hydrophobicity was determined using the bacterial adhesion to hydrocarbons (BATH) test. Exopolysaccharide profiles were determined by measuring concentrations of glucose, galactose, mannose, glucuronic, and galacturonic acids in digested exopolymer extractions using HPLC. Changes in the physical surface properties of the bacteria and the chemical composition of the extracted exopolymers were correlated with differences in the three food sources. Cell surface hydrophobicity was similar for cultures grown on different plant wastewaters, while the culture grown on synthetic food produced less floc hydrophobicity. Electrophoretic mobility measurements, charge titrations, and dye exchange titrations showed different total surface charge as well as varying charge availability. Additionally, total surface charge and total exopolysaccharide concentrations appeared less dependent on food source than the food-to-mass ratio. High concentrations of biodegradable food produced dispersed growth and high concentrations of exopolysaccharides that contributed to poor settling.

Relationship of Cell Surface Charge and Hydrophobicity to Strength of Attachment of Bacteria to Cantaloupe Rind†

2002 ◽  
Vol 65 (7) ◽  
pp. 1093-1099 ◽  
Author(s):  
DIKE O. UKUKU ◽  
WILLIAM F. FETT
Keyword(s):  
Cell Surface ◽  
Surface Charge ◽  
Cell Surface Hydrophobicity ◽  
Hydrophobic Interaction Chromatography ◽  
Surface Hydrophobicity ◽  
Bacterial Attachment ◽  
Wash Water ◽  
Bacterial Surface ◽  
E Coli ◽  
Salmonella Infections

The cantaloupe melon has been associated with outbreaks of Salmonella infections. It is suspected that bacterial surface charge and hydrophobicity may affect bacterial attachment and complicate bacterial detachment from cantaloupe surfaces. The surface charge and hydrophobicity of strains of Salmonella, Escherichia coli (O157:H7 and non-O157:H7), and Listeria monocytogenes were determined by electrostatic and hydrophobic interaction chromatography, respectively. Initial bacterial attachment to cantaloupe surfaces and the ability of bacteria to resist removal by washing with water were compared with surface charge and hydrophobicity. Whole cantaloupes were submerged in inocula containing individual strains or in cocktails containing Salmonella, E. coli, and L. monocytogenes, either as a mixture of strains containing all three genera or as a mixture of strains belonging to a single genus, for 10 min. Inoculated cantaloupes were dried for 1 h in a biosafety cabinet and then stored for up to 7 days at 4°C. Inoculated melons were washed with water, and bacteria still attached to the melon surface, as well as those in the wash water, were enumerated. Initial bacterial attachment was highest for individual strains of E. coli and lowest for L. monocytogenes, but Salmonella exhibited the strongest attachment on days 0, 3, and 7. When mixed-genus cocktails were used, the relative degrees of attachment of the three genera ware altered. The attachment of Salmonella strains was the strongest, but the attachment of E. coli was more extensive than that of L. monocytogenes on days 0, 3, and 7. There was a linear correlation between bacterial cell surface hydrophobicity (r2 = 0.767), negative charge (r2 = 0.738), and positive charge (r2 = 0.724) and the strength of bacterial attachment to cantaloupe surfaces.

Autecology of scum producing bacteria

1998 ◽  
Vol 37 (4-5) ◽  
pp. 527-530 ◽  
Author(s):  
Hilde Lemmer ◽  
George Lind ◽  
Margit Schade ◽  
Birgit Ziegelmayer
Keyword(s):  
Wastewater Treatment ◽  
Cell Surface ◽  
Wastewater Treatment Plants ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Residence Times ◽  
Loading Conditions ◽  
Emulsifying Activity ◽  
Substrate Preferences

Non-filamentous hydrophobic scum bacteria were isolated from scumming wastewater treatment plants (WWTP) by means of adhesion to hydrocarbons. They were characterized with respect to taxonomy, substrate preferences, cell surface hydrophobicity, and emulsification capability. Their role during flotation events is discussed. Rhodococci are selected by hydrolysable substrates and contribute to flotation both by cell surface hydrophobicity and emulsifying activity at long mean cell residence times (MCRT). Saprophytic Acinetobacter strains are able to promote flotation by hydrophobicity and producing emulsifying agents under conditions when hydrophobic substrates are predominant. Hydrogenophaga and Acidovorax species as well as members of the Cytophaga/Flavobacterium group are prone to proliferate under low loading conditions and contribute to flotation mainly by emulsification.

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

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