scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

Cells ◽  
2021 ◽  
Vol 10 (6) ◽  
pp. 1519
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Fluorescent Light ◽  
Fluorescent Nanoparticles ◽  
Cell Surface Charge ◽  
Surface Charge Distribution

Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle (NP) interactions. Fluorescent nanoparticles (NPs) were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescent light intensity and number of nanoparticles, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, human umbilical vein endothelial cells (HUVECs) and HeLa cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potentials of the two types of cells were obtained, which are in good agreement with the standard electrophoretic light scattering measurement. This method can be used for rapid surface charge mapping of single particles or cells, and can advance cell-surface-charge characterization applications in many biomedical fields.

scholarly journals Mapping Surface Charge Distribution of Single-Cell via Charged Nanoparticle

2021 ◽  
Author(s):  
Leixin Ouyang ◽  
Rubia Shaik ◽  
Ruiting Xu ◽  
Ge Zhang ◽  
Jiang Zhe
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Surface Charge Density ◽  
Single Cells ◽  
Cell Behaviors ◽  
Cell Surface Charge ◽  
Surface Charge Distribution ◽  
Fluorescence Light

Abstract Background: Many bio-functions of cells can be regulated by their surface charge characteristics. Mapping surface charge density in a single cell’s surface is vital to advance the understanding of cell behaviors. Results: This article demonstrates a method of cell surface charge mapping via electrostatic cell–nanoparticle interactions. Nanoparticles with fluorescence were used as the marker to investigate single cells’ surface charge distribution. The nanoparticles with opposite charges were electrostatically bonded to the cell surface; a stack of fluorescence distribution on a cell’s surface at a series of vertical distances was imaged and analyzed. By establishing a relationship between fluorescence light intensity and surface charge density, cells’ surface charge distribution was quantified from the fluorescence distribution. Two types of cells, HUVECs and Hela cells, were tested. From the measured surface charge density of a group of single cells, the average zeta potential of the two types of cells was obtained, which is in good agreement with the standard electrophoretic light scattering measurement. Conclusions: This method can be used for rapid surface charge mapping of single particles or cells and can advance cell-surface-charge characterization applications in many biomedical fields.

Nanosecond pulsed electric field induced changes in cell surface charge density

Micron ◽  
2017 ◽  
Vol 100 ◽  
pp. 45-49 ◽  
Author(s):  
Diganta Dutta ◽  
Xavier-Lewis Palmer ◽  
Anthony Asmar ◽  
Michael Stacey ◽  
Shizhi Qian
Keyword(s):  
Cell Surface ◽  
Electric Field ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Pulsed Electric Field ◽  
Cell Surface Charge ◽  
Nanosecond Pulsed Electric Field ◽  
Induced Changes

scholarly journals Direct measurement of surface charge distribution in phase separating supported lipid bilayers

Nanoscale ◽  
2018 ◽  
Vol 10 (9) ◽  
pp. 4538-4544 ◽  
Author(s):  
Thomas Fuhs ◽  
Lasse Hyldgaard Klausen ◽  
Steffan Møller Sønderskov ◽  
Xiaojun Han ◽  
Mingdong Dong
Keyword(s):  
Membrane Proteins ◽  
Cell Membrane ◽  
Charge Density ◽  
Surface Charge ◽  
Charge Distribution ◽  
Lipid Bilayers ◽  
Surface Charge Density ◽  
Supported Lipid Bilayers ◽  
Local Surface ◽  
Surface Charge Distribution

The local surface charge density of the cell membrane influences regulation and localization of membrane proteins.

Cell Electrophoresis of the Cellular Slime Mould Dictyostelium Discoideum

1972 ◽  
Vol 10 (1) ◽  
pp. 249-265
Author(s):  
K.-C. LEE
Keyword(s):  
Cell Adhesion ◽  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Cell Aggregation ◽  
Cellular Slime Mould ◽  
Cell Electrophoresis ◽  
Electrophoretic Mobilities ◽  
Cellular Slime

The reduction in the cell surface charge density of Dictyostelium discoideum during differentiation has been studied by the technique of cell electrophoresis. It was abolished under conditions in which cell aggregation was inhibited (e.g. low temperature, the presence of actinomycin D or cycloheximide). In the presence of nutrients incapable of supporting growth, cell aggregation occurred without a reduction in surface charge density. Cell adhesion in these aggregates was impaired, and a reduction in surface charge density appeared to be necessary for further development. Brief treatment of exponential phase and aggregating cells with agents which disaggregate slugs failed to alter their electrophoretic mobilities. Low concentrations of magnesium chloride caused extensive agglutination, especially in aggregating cell suspensions, but little change in their electrophoretic mobilities. Magnesium chloride could agglutinate cells by association with cell surface components undetectable by cell electrophoresis. This, together with immunological evidence from other workers, supports the possibility of involvement of specific surface macromolecules in cellular slime mould aggregation. It was concluded that changes in surface charge density, though important for cell adhesion and morphogenesis, cannot account for all aspects of cell interactions in D. discoideum.

scholarly journals CELL SURFACE CHANGES DURING DEDIFFERENTIATION IN THE METAPLASTIC TRANSFORMATION OF IRIS INTO LENS

1972 ◽  
Vol 55 (1) ◽  
pp. 134-146 ◽  
Author(s):  
Sara E. Zalik ◽  
Vi Scott
Keyword(s):  
Cell Surface ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Cell Periphery ◽  
Notophthalmus Viridescens ◽  
Taricha Granulosa ◽  
Electrophoretic Mobilities ◽  
Cell Surface Changes ◽  
Surface Changes

Changes at the cell periphery during the dedifferentiative phase of the metaplastic transformation of iris into lens have been studied in Notophthalmus viridescens and Taricha granulosa using cell electrophoresis. Cell surface charge density increases as early as 1–3 days after lens removal. Cells of regenerates at 10–15 days after lentectomy have significantly lower electrophoretic mobilities than those of the irises of nonlentectomized newts. Decrease in surface charge density is due, at least in part, to the loss of ribonuclease- and neuraminidase-sensitive groups from the cell periphery. Loss of negatively charged groups from the cell surface appears to occur as cells go through dedifferentiation. Loss of cell surface components also occurs in the cells of the ventral iris which also undergo dedifFerentiation but do not regenerate a lens.

scholarly journals Analytical Solution for the Electric Field Response Generated by a Nonconducting Ellipsoid (Prolate Spheroid) in a Conducting Fluid Subject to an External Electric Field

2020 ◽  
pp. 383-389
Author(s):  
Andrey B. Yakovlev ◽  
Valeriya S. Federyaeva
Keyword(s):  
Analytical Solution ◽  
Electric Field ◽  
Charge Density ◽  
Surface Charge ◽  
Surface Charge Density ◽  
Prolate Spheroid ◽  
Conducting Fluid ◽  
Cell Modeling ◽  
Surface Charge Distribution ◽  
Field Response

AbstractAn analytical solution is presented for the electric field response generated by a nonconducting ellipsoid (prolate spheroid) in a homogeneous conducting fluid subject to an external primary electric field, including surface charge distribution. Such a solution might be useful for different purposes, including cell modeling subject to an external quasistatic electromagnetic stimulus. The solution utilizes the well-known analogy between the electrostatics of dielectrics and DC conduction. The solution obtained includes an expression for the volumetric fields and an expression for the induced surface charge density at the membrane.

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