Gypsy moth cell lines divergent in viral susceptibility

In Vitro ◽  
1978 ◽  
Vol 14 (6) ◽  
pp. 485-494 ◽  
Author(s):  
R. H. Goodwin ◽  
G. J. Tompkins ◽  
P. McCawley
Keyword(s):  
Cell Lines ◽  
Gypsy Moth ◽  
Viral Susceptibility

Establishment, characterization and viral susceptibility of 3 new cell lines from snakehead, Channa striatus (Blooch)

2004 ◽  
Vol 25 (3-4) ◽  
pp. 155-166 ◽  
Author(s):  
Zhengshan Zhao1 ◽  
Dee Montgomery-Brock ◽  
Cheng-Sheng Lee ◽  
Yuanan Lu
Keyword(s):  
Cell Lines ◽  
Channa Striatus ◽  
Viral Susceptibility

Evaluation of TV cell line viral susceptibility using conventional cell culture techniques

Open Medicine ◽  
2006 ◽  
Vol 1 (1) ◽  
pp. 12-22 ◽  
Author(s):  
Coralia Bleotu ◽  
Carmen Diaconu ◽  
Mihaela Chivu ◽  
Irina Alexiu ◽  
Simona Ruta ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Line ◽  
Cell Lines ◽  
Virus Detection ◽  
Virus Growth ◽  
Cell Culture Techniques ◽  
Culture Techniques ◽  
Viral Susceptibility ◽  
Golden Standard ◽  
Conventional Cell

AbstractDespite the fact that a lot of methods have been developed for rapid virus detection, classic cell culture is still “the golden standard”. The range of viruses that can be isolated and cultured in cell line systems is often limited by the susceptibility of cells to support viral replication. Since the primary cell culture, the best cellular system available to support replication of a large number of viruses, is very expensive and diffcult to obtain, cell lines, which are easier to manipulate, are commonly used for virus growth and isolation.In two previous papers we described the TV cell line initiated by our team from a laryngeal tumor, which harbors human papillomavirus (HPV) gene sequences. In this paper we analyze its capacity to support virus replication. Depending on the virus, different cytopathic effects were produced. Comparison of viral effect observed on this cell line with the effect obtained on other cell lines has been performed. This cell line might be used in the clinical virology laboratory for virus isolation.

scholarly journals Lymantria dispar iflavirus 1 (LdIV1), a new model to study iflaviral persistence in lepidopterans

2014 ◽  
Vol 95 (10) ◽  
pp. 2285-2296 ◽  
Author(s):  
Jimena Carrillo-Tripp ◽  
Elizabeth N. Krueger ◽  
Robert L. Harrison ◽  
Amy L. Toth ◽  
W. Allen Miller ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Rna Virus ◽  
Acute Infection ◽  
Full Genome Sequence ◽  
New Model ◽  
Persistent Infections ◽  
Suppressor Of Rna Silencing ◽  
Persistently Infect

The cell line IPLB-LD-652Y, derived from the gypsy moth (Lymantria dispar L.), is routinely used to study interactions between viruses and insect hosts. Here we report the full genome sequence and biological characteristics of a small RNA virus, designated Lymantria dispar iflavirus 1 (LdIV1), that was discovered to persistently infect IPLB-LD-652Y. LdIV1 belongs to the genus Iflavirus. LdIV1 formed icosahedral particles of approx. 30 nm in diameter and contained a 10 044 nt polyadenylated, positive-sense RNA genome encoding a predicted polyprotein of 2980 aa. LdIV1 was induced by a viral suppressor of RNA silencing, suggesting that acute infection is restricted by RNA interference (RNAi). We detected LdIV1 in all tested tissues of gypsy-moth larvae and adults, but the virus was absent from other L. dispar-derived cell lines. We confirmed LdIV1 infectivity in two of these cell lines (IPLB-LD-652 and IPLB-LdFB). Our results provide a novel system to explore persistent infections in lepidopterans and a new model for the study of iflaviruses, a rapidly expanding group of viruses, many of which covertly infect their hosts.

scholarly journals Viral susceptibility of newly established cell lines from the Hawaiian monk seal Monachus schauinslandi

2003 ◽  
Vol 57 ◽  
pp. 183-191 ◽  
Author(s):  
Y Lu ◽  
AA Aguirre ◽  
Y Wang ◽  
L Zeng ◽  
PC Loh ◽  
...  
Keyword(s):  
Cell Lines ◽  
Hawaiian Monk Seal ◽  
Monachus Schauinslandi ◽  
Viral Susceptibility ◽  
Established Cell Lines ◽  
Established Cell

Transformation of Gypsy Moth (Lymantria dispar) Cell Lines by Infection withGlyptapanteles indiensisPolydnavirus

1996 ◽  
Vol 225 (3) ◽  
pp. 764-770 ◽  
Author(s):  
Terry A. McKelvey ◽  
Dwight E. Lynn ◽  
Dawn Gundersen-Rindal ◽  
David Guzo ◽  
Donald A. Stoltz ◽  
...  
Keyword(s):  
Cell Lines ◽  
Gypsy Moth ◽  
Lymantria Dispar
Sign in / Sign up

Export Citation Format

Share Document