Ciliated cells in vitamin A-deprived cultured hamster tracheal epithelium do divide

1988 ◽  
Vol 24 (9) ◽  
pp. 931-935 ◽  
Author(s):  
A. A. J. J. L. Rutten ◽  
R. B. Beems ◽  
J. W. G. M. Wilmer ◽  
V. J. Feron

Development ◽  
1963 ◽  
Vol 11 (3) ◽  
pp. 621-635
Author(s):  
Margaret B. Aydelotte

The effects of vitamin A and citral on the differentiation of chick tracheal epithelium in vitro were described in a previous paper (Aydelotte, 1963a). High concentrations of vitamin A inhibited the development of tracheal mucous cells but the epithelium became well ciliated. Citral in low concentrations favoured the differentiation of mucous cells but few ciliated cells developed; in higher concentrations of citral the tracheal epithelium became stratified and occasionally keratinized. The changes produced by citral resembled those in the tracheal epithelium of vitamin A deficient chicks (Aydelotte, 1963b) and when vitamin A and citral were both added to the culture medium, the combined effect was intermediate between those given by the two compounds separately. These results, therefore, supported the suggestion put forward by Leach & Lloyd (1956) that citral inhibits vitamin A. The investigation of the effects of vitamin A and citral in vitro has been extended to the oesophageal and corneal epithelia and epidermis of the chick embryo.



1987 ◽  
Vol 13 (2) ◽  
pp. 223-240 ◽  
Author(s):  
Linda N. Curtis ◽  
Johnny L. Carson ◽  
Albert M. Collier ◽  
Todd M. Gambling ◽  
S. S. Hu ◽  
...  


1995 ◽  
Vol 91 (2) ◽  
pp. 205-214 ◽  
Author(s):  
Andreas P.M. Wolterbeek ◽  
Robert Roggeband ◽  
Catherina J.A. van Moorsel ◽  
Robert A. Baan ◽  
Jan H. Koeman ◽  
...  


1974 ◽  
pp. 257-264 ◽  
Author(s):  
Curtis D. Port ◽  
David W. Baxter ◽  
Curtis C. Harris


1986 ◽  
Vol 25 (2) ◽  
pp. 114-122 ◽  
Author(s):  
H. K. Biesalski ◽  
E. Stofft ◽  
U. Wellner ◽  
U. Niederauer ◽  
K. H. Bässler






1993 ◽  
Vol 30 (1) ◽  
pp. 12-19 ◽  
Author(s):  
A. T. Hastie ◽  
L. P. Evans ◽  
A. M. Allen

Two hundred sixty tracheas were obtained from a Philadelphia abattoir under permit from the Department of Agriculture; the tracheas were excised from predominantly Holstein calves of both sexes that weighed approximately 250 kg. Tracheas were transported in normal saline to the laboratory at Thomas Jefferson University, Philadelphia, Pennsylvania. Evidence of bacteria adherent to the tracheal epithelium was found in specimens from 20/24 of these tracheas. The epithelium from each of five tracheas was placed in glutaraldehyde fixative for transmission electron microscopic examination. Epithelium from each of 12 other tracheas was placed in formaldehyde fixative for light microscopic examination. Microscopically, 13 of these 17 bovine tracheal epithelia were observed to contain bacteria located longitudinally parallel to and between cilia and microvilli of ciliated cells. Preparations of ciliary axonemes isolated from the epithelium of seven additional bovine tracheas also contained these bacteria in sections viewed by a transmission electron microscope. These bacteria had two different ultrastructural morphologies: filamentous with a trilaminar-structured cell wall and short with a thick, homogeneously stained cell wall beneath a regularly arrayed surface layer. The short bacillus had surface carbohydrates, including mannose, galactose, and N-acetylgalactosamine, identified by lectin binding. The filamentous bacillus was apparently externally deficient in these carbohydrates. Immunogold staining revealed that the filamentous bacillus was antigenically related to cilia-associated respiratory (CAR) bacillus, which has been identified in rabbit and rodent species. Significantly decreased numbers of cilia were obtained from tracheal epithelium heavily colonized by the filamentous bacilli, suggesting a pathologic change in ciliated cells.



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