Ultrastructural and immunohistochemical studies on non-ciliated cells of the tracheal epithelium of normal, phenobarbital-treated, and 3-methylcholanthrene-treated mice

1985 ◽  
Vol 240 (2) ◽  
Author(s):  
K. Ishimura ◽  
M. Usa ◽  
H. Fujita ◽  
S. Kawata ◽  
M. Okamoto ◽  
...  
Keyword(s):  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
Immunohistochemical Studies

Features of Developing Ferret Tracheal Epithelium: Ultrastructural Observations of in Vivo and in Vitro Differentiation of Ciliated Cells

1987 ◽  
Vol 13 (2) ◽  
pp. 223-240 ◽  
Author(s):  
Linda N. Curtis ◽  
Johnny L. Carson ◽  
Albert M. Collier ◽  
Todd M. Gambling ◽  
S. S. Hu ◽  
...  
Keyword(s):  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
In Vitro Differentiation

Effects of Chromates on Ciliated Cells of Rat Tracheal Epithelium

1976 ◽  
Vol 31 (2) ◽  
pp. 96-100 ◽  
Author(s):  
Marc J. Mass ◽  
Bernard P. Lane
Keyword(s):  
Tracheal Epithelium ◽  
Ciliated Cells

scholarly journals Two Types of Bacteria Adherent to Bovine Respiratory Tract Ciliated Epithelium

1993 ◽  
Vol 30 (1) ◽  
pp. 12-19 ◽  
Author(s):  
A. T. Hastie ◽  
L. P. Evans ◽  
A. M. Allen
Keyword(s):  
Cell Wall ◽  
Microscopic Examination ◽  
Lectin Binding ◽  
Electron Microscopic Examination ◽  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
Electron Microscopic ◽  
Department Of Agriculture ◽  
Immunogold Staining ◽  
Transmission Electron

Two hundred sixty tracheas were obtained from a Philadelphia abattoir under permit from the Department of Agriculture; the tracheas were excised from predominantly Holstein calves of both sexes that weighed approximately 250 kg. Tracheas were transported in normal saline to the laboratory at Thomas Jefferson University, Philadelphia, Pennsylvania. Evidence of bacteria adherent to the tracheal epithelium was found in specimens from 20/24 of these tracheas. The epithelium from each of five tracheas was placed in glutaraldehyde fixative for transmission electron microscopic examination. Epithelium from each of 12 other tracheas was placed in formaldehyde fixative for light microscopic examination. Microscopically, 13 of these 17 bovine tracheal epithelia were observed to contain bacteria located longitudinally parallel to and between cilia and microvilli of ciliated cells. Preparations of ciliary axonemes isolated from the epithelium of seven additional bovine tracheas also contained these bacteria in sections viewed by a transmission electron microscope. These bacteria had two different ultrastructural morphologies: filamentous with a trilaminar-structured cell wall and short with a thick, homogeneously stained cell wall beneath a regularly arrayed surface layer. The short bacillus had surface carbohydrates, including mannose, galactose, and N-acetylgalactosamine, identified by lectin binding. The filamentous bacillus was apparently externally deficient in these carbohydrates. Immunogold staining revealed that the filamentous bacillus was antigenically related to cilia-associated respiratory (CAR) bacillus, which has been identified in rabbit and rodent species. Significantly decreased numbers of cilia were obtained from tracheal epithelium heavily colonized by the filamentous bacilli, suggesting a pathologic change in ciliated cells.

In Utero and Postnatal Exposure of Wistar Rats to Low Frequency/High Intensity Noise Depletes the Tracheal Epithelium of Ciliated Cells

Lung ◽  
2001 ◽  
Vol 179 (4) ◽  
pp. 225-232 ◽  
Author(s):  
M.J.R. Oliveira ◽  
A.S. Pereira ◽  
N.A.A. Castelo Branco ◽  
N.R. Grande ◽  
A.P. águas
Keyword(s):  
High Intensity ◽  
Wistar Rats ◽  
Low Frequency ◽  
In Utero ◽  
Tracheal Epithelium ◽  
Intensity Noise ◽  
Ciliated Cells ◽  
Postnatal Exposure

Parainfluenza (Sendai) Virus Infects Ciliated Cells and Secretory Cells but Not Basal Cells of Rat Tracheal Epithelium

1993 ◽  
Vol 9 (4) ◽  
pp. 361-370 ◽  
Author(s):  
P. P. Massion ◽  
C. C. P. Funari ◽  
I. Ueki ◽  
S. Ikeda ◽  
D. M. McDonald ◽  
...  
Keyword(s):  
Sendai Virus ◽  
Tracheal Epithelium ◽  
Secretory Cells ◽  
Basal Cells ◽  
Ciliated Cells

Ciliated cells in vitamin A-deprived cultured hamster tracheal epithelium do divide

1988 ◽  
Vol 24 (9) ◽  
pp. 931-935 ◽  
Author(s):  
A. A. J. J. L. Rutten ◽  
R. B. Beems ◽  
J. W. G. M. Wilmer ◽  
V. J. Feron
Keyword(s):  
Vitamin A ◽  
Tracheal Epithelium ◽  
Ciliated Cells

Localization of Peroxidase Activity in Tracheal Epithelium

1980 ◽  
Vol 89 (3) ◽  
pp. 241-248 ◽  
Author(s):  
Kensuke Watanabe
Keyword(s):  
Peroxidase Activity ◽  
Secretory Granules ◽  
Goblet Cells ◽  
Tracheal Ring ◽  
Tracheal Epithelium ◽  
Basal Cells ◽  
Ciliated Cells ◽  
Cytochemical Demonstration ◽  
Apical Vesicles ◽  
Intermediate Cells

The activity of endogenous peroxidase was demonstrated in light ciliated cells, mature goblet cells, and nonciliated serous cells, but not in basal cells, intermediate cells, dark ciliated cells or immature goblet cells of the upper tracheal epithelium of rats, using the diaminobenzidine method for cytochemical demonstration of peroxidase activity. The peroxidase activity was the most intense in the epithelium of the first tracheal ring and gradually diminished at lower tracheal levels, becoming extremely faint in the vicinity of the middle of the trachea. The activity was localized in cisternae of rough-surfaced endoplasmic reticulum (r-ER) including the nuclear envelope, some vesicles and saccules of the Golgi complex, secretory granules, and small apical vesicles. It is believed that the basal cells were transformed into the intermediate cells, and some of the intermediate cells were turned into the ciliated cells, while other intermediate cells were differentiated into the goblet cells. The dark ciliated cells proved to be the immature ciliated cells immediately after the transformation from the intermediate cells. In these cells, peroxidase is not yet produced.

Effects of sulfur dioxide on pore populations of canine tracheal epithelium

1986 ◽  
Vol 60 (2) ◽  
pp. 416-426 ◽  
Author(s):  
S. F. Man ◽  
W. C. Hulbert ◽  
K. Mok ◽  
T. Ryan ◽  
A. B. Thomson
Keyword(s):  
Electron Microscopy ◽  
Scanning Electron Microscopy ◽  
Barrier Properties ◽  
The Other ◽  
Compressed Air ◽  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
High Concentrations ◽  
Scanning Electron ◽  
Nonelectrolyte Permeability

The bioelectric and barrier properties of the tracheal epithelium in nose-breathing dogs and in dogs that had been exposed for 75 min to compressed air or to two high concentrations of SO2 were measured and compared. We also studied tissues that had been treated with chloroform. Based on a model of restrictive diffusion we demonstrated heteropores (6 and 250 A) in the control tissues. Bioelectric changes due to 100-ppm SO2 were minimal. After exposure to 500 ppm SO2, adverse changes in the bioelectric properties were focal; they were marked in 8 out of 12 animals but were less striking in the other 4. Nonelectrolyte permeability increased with an increase in SO2 concentrations. Small pores were still present in the tissues severely affected by SO2 but they were absent in chloroform-treated tissues. Scanning electron microscopy of tissues from animals exposed to 500 ppm SO2 showed that in the same dog tissue appearance varied from normal to one of repair (normal bioelectric properties) or one of marked exfoliation of ciliated cells (abnormal bioelectric measurements).

Electron Microscopical Observations on the Function of the Tracheal Epithelium, with Special Reference to Ciliated Cells

ORL ◽  
1970 ◽  
Vol 32 (4) ◽  
pp. 202-210
Author(s):  
Y. Nakai ◽  
Y. Nagae
Keyword(s):  
Special Reference ◽  
Tracheal Epithelium ◽  
Ciliated Cells ◽  
Electron Microscopical
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