Sterol metabolism and oral epithelial cell growth

1993 ◽  
Vol 29 (9) ◽  
pp. 693-698 ◽  
Author(s):  
Gretchen B. Caughman ◽  
George S. Schuster ◽  
Thomas R. Dirksen
Keyword(s):  
Cell Growth ◽  
Epithelial Cell ◽  
Sterol Metabolism ◽  
Oral Epithelial Cell ◽  
Oral Epithelial

Evaluation of Buccal Methyl-β-Cyclodextrin Toxicity on Human Oral Epithelial Cell Culture Model

2005 ◽  
Vol 94 (6) ◽  
pp. 1300-1309 ◽  
Author(s):  
Laïla Boulmedarat ◽  
Amélie Bochot ◽  
Sylviane Lesieur ◽  
Elias Fattal
Keyword(s):  
Cell Culture ◽  
Epithelial Cell ◽  
Cell Culture Model ◽  
Epithelial Cell Culture ◽  
Oral Epithelial Cell ◽  
Culture Model ◽  
Oral Epithelial ◽  
Human Oral Epithelial Cell

Characteristics of a cancerous cell line, HIOEC-B(a)P-96, induced by benzo(a)pyrene from human immortalized oral epithelial cell line

2008 ◽  
Vol 53 (5) ◽  
pp. 443-452 ◽  
Author(s):  
Lai-Ping Zhong ◽  
Hong-Ya Pan ◽  
Xiao-Jian Zhou ◽  
Dong-Xia Ye ◽  
Lei Zhang ◽  
...  
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Epithelial Cell Line ◽  
Oral Epithelial Cell ◽  
Cancerous Cell ◽  
Oral Epithelial

scholarly journals Oral Epithelial Cell Responses to Multispecies Microbial Biofilms

2013 ◽  
Vol 92 (3) ◽  
pp. 235-240 ◽  
Author(s):  
R. Peyyala ◽  
S.S. Kirakodu ◽  
K.F. Novak ◽  
J.L Ebersole
Keyword(s):  
Epithelial Cell ◽  
Cell Responses ◽  
Oral Epithelial Cell ◽  
Microbial Biofilms ◽  
Oral Epithelial

scholarly journals Self-Collection of Oral Epithelial Cell DNA under Instruction from Epidemiologic Interviewers

2000 ◽  
Vol 151 (2) ◽  
pp. 199-205 ◽  
Author(s):  
L. C. Harty ◽  
P. G. Shields ◽  
D. M. Winn ◽  
N. E. Caporaso ◽  
R. B. Hayes
Keyword(s):  
Epithelial Cell ◽  
Oral Epithelial Cell ◽  
Oral Epithelial

scholarly journals Potential Role for a Carbohydrate Moiety in Anti-Candida Activity of Human Oral Epithelial Cells

2001 ◽  
Vol 69 (11) ◽  
pp. 7091-7099 ◽  
Author(s):  
Chad Steele ◽  
Janet Leigh ◽  
Rolf Swoboda ◽  
Hatice Ozenci ◽  
Paul L. Fidel
Keyword(s):  
Epithelial Cell ◽  
Epithelial Cells ◽  
Oral Mucosa ◽  
Host Defense ◽  
Defense Mechanisms ◽  
Carbohydrate Moiety ◽  
Sulfated Polysaccharides ◽  
Oral Epithelial Cells ◽  
Oral Epithelial Cell ◽  
Oral Epithelial

ABSTRACT Candida albicans is both a commensal and a pathogen at the oral mucosa. Although an intricate network of host defense mechanisms are expected for protection against oropharyngeal candidiasis, anti-Candida host defense mechanisms at the oral mucosa are poorly understood. Our laboratory recently showed that primary epithelial cells from human oral mucosa, as well as an oral epithelial cell line, inhibit the growth of blastoconidia and/or hyphal phases of several Candida species in vitro with a requirement for cell contact and with no demonstrable role for soluble factors. In the present study, we show that oral epithelial cell-mediated anti-Candida activity is resistant to gamma-irradiation and is not mediated by phagocytosis, nitric oxide, hydrogen peroxide, and superoxide oxidative inhibitory pathways or by nonoxidative components such as soluble defensin and calprotectin peptides. In contrast, epithelial cell-mediated anti-Candida activity was sensitive to heat, paraformaldehyde fixation, and detergents, but these treatments were accompanied by a significant loss in epithelial cell viability. Treatments that removed existing membrane protein or lipid moieties in the presence or absence of protein synthesis inhibitors had no effect on epithelial cell inhibitory activity. In contrast, the epithelial cell-mediated anti-Candida activity was abrogated after treatment of the epithelial cells with periodic acid, suggesting a role for carbohydrates. Adherence of C. albicans to oral epithelial cells was unaffected, indicating that the carbohydrate moiety is exclusively associated with the growth inhibition activity. Subsequent studies that evaluated specific membrane carbohydrate moieties, however, showed no role for sulfated polysaccharides, sialic acid residues, or glucose- and mannose-containing carbohydrates. These results suggest that oral epithelial cell-mediated anti-Candida activity occurs exclusively with viable epithelial cells through contact with C. albicans by an as-yet-undefined carbohydrate moiety.

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