Studies on the structure and function of 16S ribosomal RNA using structure-specific chemical probes

1985 ◽  
Vol 8 (3-4) ◽  
pp. 747-755 ◽  
Author(s):  
Harry F. Noller ◽  
Barbara J. Van Stolk ◽  
Danesh Moazed ◽  
Stephen Douthwaite ◽  
Robin R. Gutell
Keyword(s):  
Ribosomal Rna ◽  
Structure And Function ◽  
16S Ribosomal Rna ◽  
Chemical Probes ◽  
Specific Chemical ◽  
And Function

scholarly journals Structure and biogenesis of small nucleolar RNAs acting as guides for ribosomal RNA modification.

1999 ◽  
Vol 46 (2) ◽  
pp. 377-389 ◽  
Author(s):  
W Filipowicz ◽  
P Pelczar ◽  
V Pogacic ◽  
F Dragon
Keyword(s):  
Ribosomal Rna ◽  
Structure And Function ◽  
Hydroxyl Group ◽  
Rna Modification ◽  
Small Nucleolar Rnas ◽  
Yeast Saccharomyces Cerevisiae ◽  
Site Specific ◽  
Alternative Pathways ◽  
And Function ◽  
Nucleolar Rnas

Maturation of pre-ribosomal RNA (pre-rRNA) in eukaryotic cells takes place in the nucleolus and involves a large number of cleavage events, which frequently follow alternative pathways. In addition, rRNAs are extensively modified, with the methylation of the 2'-hydroxyl group of sugar residues and conversion of uridines to pseudouridines being the most frequent modifications. Both cleavage and modification reactions of pre-rRNAs are assisted by a variety of small nucleolar RNAs (snoRNAs), which function in the form of ribonucleoprotein particles (snoRNPs). The majority of snoRNAs acts as guides directing site-specific 2'-O-ribose methylation or pseudouridine formation. Over one hundred RNAs of this type have been identified to date in vertebrates and the yeast Saccharomyces cerevisiae. This number is readily explained by the findings that one snoRNA acts as a guide usually for one or at most two modifications, and human rRNAs contain 91 pseudouridines and 106 2'-O-methyl residues. In this article we review information about the biogenesis, structure and function of guide snoRNAs.

scholarly journals Design and Construction of a Focused DNA-Encoded Library for Multivalent Chromatin Reader Proteins

Molecules ◽  
2020 ◽  
Vol 25 (4) ◽  
pp. 979 ◽  
Author(s):  
Justin M. Rectenwald ◽  
Shiva Krishna Reddy Guduru ◽  
Zhao Dang ◽  
Leonard B. Collins ◽  
Yi-En Liao ◽  
...  
Keyword(s):  
Structure And Function ◽  
Cellular Phenotype ◽  
Chemical Probes ◽  
Target Class ◽  
High Quality ◽  
Histone Tails ◽  
Post Translational Modifications ◽  
Chromatin Dynamics ◽  
And Function ◽  
Insight Into

Chromatin structure and function, and consequently cellular phenotype, is regulated in part by a network of chromatin-modifying enzymes that place post-translational modifications (PTMs) on histone tails. These marks serve as recruitment sites for other chromatin regulatory complexes that ‘read’ these PTMs. High-quality chemical probes that can block reader functions of proteins involved in chromatin regulation are important tools to improve our understanding of pathways involved in chromatin dynamics. Insight into the intricate system of chromatin PTMs and their context within the epigenome is also therapeutically important as misregulation of this complex system is implicated in numerous human diseases. Using computational methods, along with structure-based knowledge, we have designed and constructed a focused DNA-Encoded Library (DEL) containing approximately 60,000 compounds targeting bi-valent methyl-lysine (Kme) reader domains. Additionally, we have constructed DNA-barcoded control compounds to allow optimization of selection conditions using a model Kme reader domain. We anticipate that this target-class focused approach will serve as a new method for rapid discovery of inhibitors for multivalent chromatin reader domains.

Sign in / Sign up

Export Citation Format

Share Document