Modification of lipase fromCandida rugosa with poly(ethylene oxide-co-maleic anhydride) and its separation using aqueous two-phase partition system

Maleic anhydride modified polycarboxylates superplasticizes (MPC) was synthesized from allylic poly (ethylene oxide ether) (APEG), maleic anhydride (MAH), sodium methylallyl sulfonate (SMAS) using ammonium persulfate (APS) as initiator. The optimal MPC with best fluidity was got at 90 °C when molar ratio of MAH, APEG, SAMA was 2.75∶1∶0.4 in the present of 4.5 mol% APS of all the monomer molars. MPC which possessed hydroxyl terminated side chains shows some difference in fluidity retarding related to the conventional superplasticizers possessed methoxy terminated side chains.

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Remediation of PAH-contaminated soil at a gas manufacturing plant by a combined two-phase partition system washing and microbial degradation process

Environmental Science and Pollution Research ◽

10.1007/s11356-015-4466-y ◽

2015 ◽

Vol 22 (16) ◽

pp. 12001-12010 ◽

Cited By ~ 17

Author(s):

Xuan Gong ◽

Xinyang Xu ◽

Zongqiang Gong ◽

Xiaojun Li ◽

Chunyun Jia ◽

...

Keyword(s):

Microbial Degradation ◽

Contaminated Soil ◽

Degradation Process ◽

Manufacturing Plant ◽

Two Phase ◽

Partition System ◽

Phase Partition ◽

Two Phase Partition

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The dynamics of phase partition. A study of parameters affecting rat liver organelle partitioning in aqueous two-polymer phase systems

Biochemical Journal ◽

10.1042/bj2350245 ◽

1986 ◽

Vol 235 (1) ◽

pp. 245-249 ◽

Cited By ~ 1

Author(s):

D Heywood-Waddington ◽

T J Peters ◽

I A Sutherland

Keyword(s):

Rat Liver ◽

Dynamic Process ◽

Volume Ratio ◽

Theoretical Modelling ◽

Two Phase ◽

Phase Mixing ◽

Phase Partition ◽

Poly Ethylene ◽

Two Phases ◽

Two Phase Partition

Separation of subcellular organelles by two-phase partition is thought to reflect differential partition of the organelles between the two phases or between one of the phases and the interface. Studies by Fisher and colleagues [Fisher & Walter (1984) Biochim. Biophys. Acta 801, 106-110] suggest that cell separation by phase partition is a dynamic process in which the partition changes with time. This is mainly due to association of the cells with sedimenting droplets of one phase in the bulk of the other. Rat liver organelle partition was studied to determine whether the same dynamic behaviour is observed. Partition was clearly time-dependent during 24 h at unit gravity, and was also affected by altering the volume ratio of the two phases and the duration of phase mixing. These results indicate that, as with cells, the partition of organelles between phases is a dynamic process, and is consistent with the demonstration that organelles adhere to the phase droplet surfaces. Optimization of the volume ratio between phases may lead to significant processing economies. Organelle sedimentation in the upper phase was significantly faster than in the isoosmotic sucrose. Theoretical modelling of apparent organelle sizes indicates that aggregation occurs in the poly(ethylene glycol)-rich upper phase. This phenomenon is likely to limit the use of this technique in organelle separations unless means can be found to decrease aggregation.

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