Remediation of PAH-contaminated soil at a gas manufacturing plant by a combined two-phase partition system washing and microbial degradation process

2015 ◽  
Vol 22 (16) ◽  
pp. 12001-12010 ◽  
Author(s):  
Xuan Gong ◽  
Xinyang Xu ◽  
Zongqiang Gong ◽  
Xiaojun Li ◽  
Chunyun Jia ◽  
...  
Keyword(s):  
Microbial Degradation ◽  
Contaminated Soil ◽  
Degradation Process ◽  
Manufacturing Plant ◽  
Two Phase ◽  
Partition System ◽  
Phase Partition ◽  
Two Phase Partition

Cresolase activity of potato tuber partially purified in a two-phase partition system

1993 ◽  
Vol 41 (8) ◽  
pp. 1225-1228 ◽  
Author(s):  
Alvaro. Sanchez-Ferrer ◽  
Francisco. Laveda ◽  
Francisco. Garcia-Carmona
Keyword(s):  
Potato Tuber ◽  
Two Phase ◽  
Partition System ◽  
Phase Partition ◽  
Two Phase Partition

scholarly journals Electrostatic interactions in the heparin-enhanced reaction between human thrombin and antithrombin

1983 ◽  
Vol 211 (1) ◽  
pp. 91-97 ◽  
Author(s):  
L C Petersen ◽  
M Jørgensen
Keyword(s):  
Electrostatic Interactions ◽  
Reaction Rate ◽  
Peptide Substrate ◽  
Binding Studies ◽  
Two Phase ◽  
Partition System ◽  
Phase Partition ◽  
Human Thrombin ◽  
Two Phase Partition ◽  
Kinetics Of

Binding of heparin to thrombin is monitored by means of an aqueous two-phase partition system, and binding of heparin to antithrombin is monitored by means of heparin induced enhancement of the intrinsic fluorescence of the protein. Both types of binding are studied at various electrolyte compositions of the medium. Heparin is displaced from thrombin at lower concentrations of electrolyte than those necessary for its displacement from antithrombin. K+ is more efficient than Na+, which is again more efficient than Li+ in displacing heparin from these proteins. The kinetics of the reaction between thrombin and antithrombin in the presence of heparin were studied by using an assay where synthetic peptide substrate is present in the reaction mixture during the reaction between proteinase and inhibitor. The kinetics are studied at various electrolyte compositions of the medium and the results are compared with those obtained from the binding studies performed under similar conditions. The results are consistent with a model where binding of heparin to antithrombin causes enhancement of the reaction rate, and where this enhancement is abolished again when additional binding of heparin to thrombin takes place on further addition of heparin.

The application of aqueous two-phase partition to the study of chick limb mesenchymal diversification

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 267-275
Author(s):  
C. P. Cottrill ◽  
Paul T. Sharpe ◽  
Lewis Wolpert
Keyword(s):  
Pattern Formation ◽  
Limb Development ◽  
Developmental Stages ◽  
Proximal Region ◽  
Limb Bud ◽  
Cell Populations ◽  
Two Phase ◽  
Phase Partition ◽  
Surface Character ◽  
Two Phase Partition

A technique which identifies cells differing in surface character, aqueous two-phase partition using thin-layer countercurrent distribution (TLCCD), has been used to study differentiation and pattern formation in the developing chick limb bud. The TLCCD profiles of cell populations, derived from various regions of morphologically undifferentiated mesenchyme from three different stages of limb development, have been compared. At no stage, or location, has the population been found to be homogeneous. Cells from progress zones and more proximal regions could all be resolved into several populations. The populations from progress zones at three different developmental stages were qualitatively similar but differed in the proportions of cells in each. The most striking differences in cell populations were those obtained from the most proximal region of the limb, closest to the flank, which represents the developmentally most advanced region.

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