Quantification of fluorescent molecules in heterogeneous media by use of the fluorescence decay amplitude analysis

1998 ◽  
Vol 8 (1) ◽  
pp. 27-34 ◽  
Author(s):  
G. E. Dobretsov ◽  
T. I. Syrejshchikova ◽  
Yu. A. Gryzunov ◽  
M. N. Yakimenko
Keyword(s):  
Heterogeneous Media ◽  
Decay Amplitude ◽  
Fluorescence Decay ◽  
Amplitude Analysis ◽  
Fluorescent Molecules

scholarly journals Fluorescence background discrimination by prebleaching.

1979 ◽  
Vol 27 (1) ◽  
pp. 96-101 ◽  
Author(s):  
T Hirschfeld
Keyword(s):  
Fluorescent Dyes ◽  
Fluorescence Decay ◽  
Free Form ◽  
Alternative Procedure ◽  
Background Fluorescence ◽  
Decay Lifetime ◽  
Fluorescent Molecules ◽  
Background Contrast

A number of electrooptical techniques are described that discriminate against background fluorescence in biologic staining, whether from sample background or unbound excess stain. These techniques are based on the fluorescent decay lifetime difference between bound stain and the sample background or between the bound stain its free form. The fluorescence decay lifetimes may be measured either directly or in a combination gated photometry scheme to substantially enhance the sample background contrast. An alternative procedure uses the photochemical bleaching of fluorescent dyes under intense exposure to time discriminate with higher selectivity, sensitivity and in a more convenient fashion between diverse fluorescent molecules.

scholarly journals Vector-Tensor and Vector-Vector Decay Amplitude Analysis ofB0→φK*0

2007 ◽  
Vol 98 (5) ◽  
Author(s):  
B. Aubert ◽  
M. Bona ◽  
D. Boutigny ◽  
F. Couderc ◽  
Y. Karyotakis ◽  
...  
Keyword(s):  
Decay Amplitude ◽  
Amplitude Analysis

Multiples: Signal or noise?

Geophysics ◽  
2016 ◽  
Vol 81 (4) ◽  
pp. V283-V302 ◽  
Author(s):  
Arthur B. Weglein
Keyword(s):  
High Frequency ◽  
Heterogeneous Media ◽  
Velocity Model ◽  
Structural Determination ◽  
Subsurface Structure ◽  
Amplitude Analysis ◽  
Migration Method ◽  
And Migration ◽  
And Inversion

Migration and migration inversion are the seismic processing methods for structural determination and subsequent amplitude analysis, respectively. To date, the most well-founded and physically interpretable migration method is based on predicting a coincident source and receiver experiment at depth at time equals zero. We have extended that migration method for heterogeneous media and to accommodate two-way propagation in a local sense at every point from the source to the target reflector and back from the reflector to the receiver and in a global sense, separately for each of the two legs from the source to the reflector and from the reflector to the receiver. That provides the first migration method that avoids high-frequency assumptions in the imaging principle and how it is implemented, and hence, it is equally effective at all frequencies at the target or reservoir. This advance for two-way wave propagation migration then provides a tool to quantitatively, unambiguously, and definitively define the role of primaries and multiples in migration. Our conclusion was that with data consisting of primaries and multiples, for an accurate discontinuous velocity model, only primaries contribute to migration with the same image and inversion results independent of whether multiples are kept or removed. However, for a smooth and continuous velocity model (i.e., generally assumed in practice), every multiple will result in a false, misleading, and potentially injurious subsurface image and hence must be removed before migration. In practice, we migrate with a smooth velocity model, and hence multiples must be removed. When the collection of primaries is incomplete, a multiple can be used to provide an approximate image of an unrecorded primary. However, it is always the migration of primaries that provides subsurface structure and amplitude information.

Probing the Structural Features and the Micro-heterogeneity of Various Deep Eutectic Solvents and their Water Dilutions by the Photophysical Behaviour of Two Fluorophores

2020 ◽  
Author(s):  
Matteo Tiecco ◽  
Irene Di Guida ◽  
Pier Luigi Gentili ◽  
Raimondo Germani ◽  
Carmela Bonaccorso ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Transient Absorption ◽  
Photophysical Properties ◽  
Deep Eutectic Solvents ◽  
Structural Features ◽  
Solvation Dynamics ◽  
Structured Systems ◽  
Reduced Viscosity ◽  
Bond Network ◽  
Fluorescent Molecules

<div><div><div><p>The structural features of a series of diverse Deep Eutectic Solvents (DESs) have been investigated and characterized by means of two fluorescent probes. The spectral and photophysical properties of the latter are strictly dependent on the experienced environment, so that they can provide insights into the polarity, viscosity, hydrogen-bond network, and micro-heterogeneity of the various DESs.</p><p>In fact, the investigated DESs exhibit a variety of properties with regards to their hydrophilicity, acidity, and hydrogen-bond ability, and these details were deeply probed by the two fluorescent molecules. The effect of the addition of water, which is a key strategy for tuning the properties of these structured systems, was also tested. In particular, the excited state dynamics of the probes, measured by femtosecond-resolved transient absorption, proved instrumental in understanding the changes in the structural properties of the DESs, namely reduced viscosity and enhanced heterogeneity, as the water percentage increases. Differences between the various DESs in terms of both local microheterogeneity and bulk viscosity also emerged from the peculiar multi-exponential solvation dynamics undergone by the excited states of the probes.</p></div></div></div>

Probing the Structural Features and the Micro-heterogeneity of Various Deep Eutectic Solvents and their Water Dilutions by the Photophysical Behaviour of Two Fluorophores

2020 ◽  
Author(s):  
Matteo Tiecco ◽  
Irene Di Guida ◽  
Pier Luigi Gentili ◽  
Raimondo Germani ◽  
Carmela Bonaccorso ◽  
...  
Keyword(s):  
Hydrogen Bond ◽  
Transient Absorption ◽  
Photophysical Properties ◽  
Deep Eutectic Solvents ◽  
Structural Features ◽  
Solvation Dynamics ◽  
Structured Systems ◽  
Reduced Viscosity ◽  
Bond Network ◽  
Fluorescent Molecules

<div><div><div><p>The structural features of a series of diverse Deep Eutectic Solvents (DESs) have been investigated and characterized by means of two fluorescent probes. The spectral and photophysical properties of the latter are strictly dependent on the experienced environment, so that they can provide insights into the polarity, viscosity, hydrogen-bond network, and micro-heterogeneity of the various DESs.</p><p>In fact, the investigated DESs exhibit a variety of properties with regards to their hydrophilicity, acidity, and hydrogen-bond ability, and these details were deeply probed by the two fluorescent molecules. The effect of the addition of water, which is a key strategy for tuning the properties of these structured systems, was also tested. In particular, the excited state dynamics of the probes, measured by femtosecond-resolved transient absorption, proved instrumental in understanding the changes in the structural properties of the DESs, namely reduced viscosity and enhanced heterogeneity, as the water percentage increases. Differences between the various DESs in terms of both local microheterogeneity and bulk viscosity also emerged from the peculiar multi-exponential solvation dynamics undergone by the excited states of the probes.</p></div></div></div>

Vibroacoustic dynamics of heterogeneous media and NPP structures

2014 ◽  
Vol 2014 (1) ◽  
pp. 99-110
Author(s):  
Vladimir Sergeevich Fedotovskij ◽  
Tat’yana Nikolaevna Vereschagina ◽  
Svetlana Valer’evna Lunina ◽  
Evgeniya Aleksandrovna Ivanova
Keyword(s):  
Heterogeneous Media
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