Characterization of xylitol dehydrogenase from debaryomyces hansenii

1996 ◽  
Vol 56 (1) ◽  
pp. 79-87 ◽  
Author(s):  
Francisco M. Gírio ◽  
Fátima Pelica ◽  
M. T. Amaral-Collaço
Keyword(s):  
Debaryomyces Hansenii ◽  
Xylitol Dehydrogenase

Characterization of a xylitol dehydrogenase and a d -arabitol dehydrogenase from the thermo- and acidophilic red alga Galdieria sulphuraria

Planta ◽  
1997 ◽  
Vol 202 (4) ◽  
pp. 487-493 ◽  
Author(s):  
Robert Stein ◽  
Wolfgang Gross ◽  
Claus Schnarrenberger
Keyword(s):  
Red Alga ◽  
Xylitol Dehydrogenase ◽  
Galdieria Sulphuraria

scholarly journals Identification and Characterization of Oleaginous Yeast Isolated from Kefir and Its Ability to Accumulate Intracellular Fats in Deproteinated Potato Wastewater with Different Carbon Sources

2017 ◽  
Vol 2017 ◽  
pp. 1-19 ◽  
Author(s):  
Iwona Gientka ◽  
Marek Kieliszek ◽  
Karolina Jermacz ◽  
Stanisław Błażejak
Keyword(s):  
Fatty Acids ◽  
Fatty Acid ◽  
Fossil Fuels ◽  
Unsaturated Fatty Acids ◽  
Saturated Fatty Acids ◽  
Carbon Sources ◽  
Debaryomyces Hansenii ◽  
Industry Waste ◽  
Identification And Characterization

The search for efficient oleaginous microorganisms, which can be an alternative to fossil fuels and biofuels obtained from oilseed crops, has been going on for many years. The suitability of microorganisms in this regard is determined by their ability to biosynthesize lipids with preferred fatty acid profile along with the concurrent utilization of energy-rich industrial waste. In this study, we isolated, characterized, and identified kefir yeast strains using molecular biology techniques. The yeast isolates identified wereCandida inconspicua,Debaryomyces hansenii,Kluyveromyces marxianus,Kazachstania unispora, andZygotorulaspora florentina. We showed that deproteinated potato wastewater, a starch processing industry waste, supplemented with various carbon sources, including lactose and glycerol, is a suitable medium for the growth of yeast, which allows an accumulation of over 20% of lipid substances in its cells. Fatty acid composition primarily depended on the yeast strain and the carbon source used, and, based on our results, most of the strains met the criteria required for the production of biodiesel. In particular, this concerns a significant share of saturated fatty acids, such as C16:0 and C18:0, and unsaturated fatty acids, such as C18:1 and C18:2. The highest efficiency in lipid biosynthesis exceeded 6.3 g L−1.Kazachstania unisporawas able to accumulate the high amount of palmitoleic acid.

Molecular cloning, characterization, and engineering of xylitol dehydrogenase from Debaryomyces hansenii

2012 ◽  
Vol 97 (4) ◽  
pp. 1613-1623 ◽  
Author(s):  
Dipanwita Biswas ◽  
Manish Datt ◽  
Monika Aggarwal ◽  
Alok K. Mondal
Keyword(s):  
Molecular Cloning ◽  
Debaryomyces Hansenii ◽  
Xylitol Dehydrogenase

scholarly journals Characterization of a Salt-induced DhSSA Gene from the Extreme Halophilic Yeast Debaryomyces hansenii

2014 ◽  
Vol 2 (3) ◽  
pp. 76-83
Author(s):  
Hsiu-Fung Chao ◽  
Yi-Fang Hsu ◽  
Li-Tine Wang ◽  
Yung-Fu Yen
Keyword(s):  
Debaryomyces Hansenii

scholarly journals A Physiological Characterization in Controlled Bioreactors Reveals a Novel Survival Strategy for Debaryomyces hansenii at High Salinity and Confirms its Halophilic Behavior

2020 ◽  
Author(s):  
Clara Navarrete ◽  
August T. Frost ◽  
Laura Ramos-Moreno ◽  
Mette R. Krum ◽  
José L. Martínez
Keyword(s):  
Carbon Metabolism ◽  
Debaryomyces Hansenii ◽  
Model Organism ◽  
Central Carbon Metabolism ◽  
Culture Conditions ◽  
Physiological Characterization ◽  
Behavioural Patterns ◽  
Central Carbon ◽  
First Time

AbstractDebaryomyces hansenii is traditionally described as a halotolerant non-conventional yeast, being the model organism for the study of osmo- and salt tolerance mechanisms in eukaryotic systems for the past 30 years.However, unravelling of D. hansenii’s biotechnological potential has always been difficult due to the persistent limitations in the availability of efficient molecular tools described for this yeast. Additionally, there is a lack of consensus and contradictory information along the recent years that limits a comprehensive understanding of its central carbon metabolism, mainly due to a lack of physiological studies in controlled and monitored environments. Moreover, there is controversy about the diversity in the culture conditions (media composition, temperature and pH among others) used by different groups, which makes it complicated when trying to get significant conclusions and behavioural patterns.In this work, we present for the first time a physiological characterization of D. hansenii in batch cultivations using highly instrumented and controlled lab-scale bioreactors. Our findings contribute to a more complete picture of the central carbon metabolism and the external pH influence on the yeast ability to tolerate high Na+ and K+ concentrations. Finally, the controversial halophilic/halotolerant character of this yeast is further clarified.

Characterization of vinasses from five certified brands of origin (CBO) and use as economic nutrient for the xylitol production by Debaryomyces hansenii

2010 ◽  
Vol 101 (7) ◽  
pp. 2379-2388 ◽  
Author(s):  
José Manuel Salgado ◽  
Elena Martínez Carballo ◽  
Belén Max ◽  
José Manuel Domínguez
Keyword(s):  
Debaryomyces Hansenii ◽  
Xylitol Production

Production of l-xylulose from xylitol by a newly isolated strain of Bacillus pallidus Y25 and characterization of its relevant enzyme xylitol dehydrogenase

2007 ◽  
Vol 40 (5) ◽  
pp. 1206-1212 ◽  
Author(s):  
Wayoon Poonperm ◽  
Goro Takata ◽  
Kenji Morimoto ◽  
Tom Birger Granström ◽  
Ken Izumori
Keyword(s):  
Xylitol Dehydrogenase

Characterization of recombinant xylitol dehydrogenase from Galactocandida mastotermitis expressed in Escherichia coli

2003 ◽  
Vol 143-144 ◽  
pp. 533-542 ◽  
Author(s):  
Bernd Nidetzky ◽  
Heidemarie Helmer ◽  
Mario Klimacek ◽  
Regina Lunzer ◽  
Gerhard Mayer
Keyword(s):  
Escherichia Coli ◽  
Xylitol Dehydrogenase
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