Effect of incorrectly estimated parameters on the control of specific growth rate inE. coli fed-batch fermentation

1996 ◽  
Vol 1 (1) ◽  
pp. 22-25
Author(s):  
Tai Hyun Park ◽  
Sung Kwan Yoon ◽  
Whan Koo Kang
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Batch Fermentation ◽  
Specific Growth ◽  
Fed Batch ◽  
Estimated Parameters ◽  
Fed Batch Fermentation

scholarly journals Controlling specific growth rate for recombinant protein production by Pichia pastoris under oxidation stress in fed-batch fermentation

2021 ◽  
Author(s):  
Rongkang Hu ◽  
Ruiguo Cui ◽  
Qingqing Xu ◽  
Dongming Lan ◽  
Yonghua Wang
Keyword(s):  
Oxidative Stress ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Batch Fermentation ◽  
Protein Production ◽  
Specific Growth ◽  
Induction Phase ◽  
Fed Batch ◽  
Ros Accumulation ◽  
Fed Batch Fermentation

Abstract Methanol can be used by Pichia pastoris as the sole carbon source and inducer to produce recombinant proteins in high-cell-density fermentations, but also damages cells due to reactive oxygen species (ROS) accumulation from methanol oxidation. Here, we study the relationship between methanol feeding and ROS accumulation by controlling specific growth rate during the induction phase. A higher specific growth rate increased the level of ROS accumulation caused by methanol oxidation. While the cell growth rate was proportional to specific growth rate, but maximum total protein production and highest enzyme activity were achieved at a specific growth rate of 0.05 1/h as compared to that 0.065 1/h. Moreover, oxidative damage induced by over-accumulation of ROS in P. pastoris during the methanol induction phase caused cell death and reduced protein expression ability. ROS scavenging system analysis reveals that the higher specific growth rate, especially 0.065 1/h, resulted in increased intracellular catalase activity and decreased glutathione content significantly. Finally, Spearman's correlation analysis further reveals that the reduced glutathione might be beneficial for maintaining cell viability and increasing protein production under oxidative stress caused by ROS toxic accumulation. Our findings suggest an integrated strategy to control the feeding of the essential substrate based on analyzing its response to oxidative stress caused by ROS toxic accumulation, as well as develop a strategy to optimize fed-batch fermentation.

scholarly journals Controlling specific growth rate for recombinant protein production by Pichia pastoris under oxidation stress in fed-batch fermentation

2021 ◽  
Author(s):  
Rongkang Hu ◽  
Ruiguo Cui ◽  
Qingqing Xu ◽  
Dongming Lan ◽  
Yonghua Wang
Keyword(s):  
Oxidative Stress ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Batch Fermentation ◽  
Protein Production ◽  
Specific Growth ◽  
Induction Phase ◽  
Fed Batch ◽  
Ros Accumulation ◽  
Fed Batch Fermentation

Abstract Methanol can be used by Pichia pastoris as the sole carbon source and inducer to produce recombinant proteins in high-cell-density fermentations, but also damages cells due to reactive oxygen species (ROS) accumulation from methanol oxidation. Here, we study the relationship between methanol feeding and ROS accumulation by controlling specific growth rate during the induction phase. A higher specific growth rate increased the level of ROS accumulation caused by methanol oxidation. While the cell growth rate was proportional to specific growth rate, but maximum total protein production and highest enzyme activity were achieved at a specific growth rate of 0.05 1/h as compared to that 0.065 1/h. Moreover, oxidative damage induced by over-accumulation of ROS in P. pastoris during the methanol induction phase caused cell death and reduced protein expression ability. ROS scavenging system analysis reveals that the higher specific growth rate, especially 0.065 1/h, resulted in increased intracellular catalase activity and decreased glutathione content significantly. Finally, Spearman's correlation analysis further reveals that the reduced glutathione might be beneficial for maintaining cell viability and increasing protein production under oxidative stress caused by ROS toxic accumulation. Our findings suggest an integrated strategy to control the feeding of the essential substrate based on analyzing its response to oxidative stress caused by ROS toxic accumulation, as well as develop a strategy to optimize fed-batch fermentation.

scholarly journals Fed-batch fermentation of GM-CSF-producing glycoengineered Pichia pastoris under controlled specific growth rate

2010 ◽  
Vol 9 (1) ◽  
pp. 93 ◽  
Author(s):  
Pieter P Jacobs ◽  
Mehmet Inan ◽  
Nele Festjens ◽  
Jurgen Haustraete ◽  
Annelies Van Hecke ◽  
...  
Keyword(s):  
Growth Rate ◽  
Pichia Pastoris ◽  
Specific Growth Rate ◽  
Batch Fermentation ◽  
Specific Growth ◽  
Fed Batch ◽  
Gm Csf ◽  
Fed Batch Fermentation ◽  
Controlled Specific Growth Rate

Influence of specific growth rate over the secretory expression of recombinant potato carboxypeptidase inhibitor in fed-batch cultures of Escherichia coli

2010 ◽  
Vol 45 (8) ◽  
pp. 1334-1341 ◽  
Author(s):  
Juan-Miguel Puertas ◽  
Jordi Ruiz ◽  
Mónica Rodríguez de la Vega ◽  
Julia Lorenzo ◽  
Glòria Caminal ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Fed Batch ◽  
Secretory Expression ◽  
Batch Cultures ◽  
Carboxypeptidase Inhibitor

An algorithm for operating a fed-batch fermentor at optimum specific-growth rate

1989 ◽  
Vol 33 (1) ◽  
pp. 115-125 ◽  
Author(s):  
Pramod Agrawal ◽  
George Koshy ◽  
Michael Ramseier
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Specific Growth ◽  
Fed Batch

scholarly journals Specific Growth Rate control in Fed-Batch Bakers' Yeast Fermentation

1993 ◽  
Vol 26 (2) ◽  
pp. 185-188
Author(s):  
M. Keulers ◽  
L. Ariaans ◽  
M. Giuseppin ◽  
R. Soeterboek
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Rate Control ◽  
Specific Growth ◽  
Yeast Fermentation ◽  
Fed Batch ◽  
Growth Rate Control

Automated fed-batch culture of Kluyveromyces fragilis based on a novel method for on-line estimation of cell specific growth rate

2001 ◽  
Vol 9 (3) ◽  
pp. 221-231 ◽  
Author(s):  
Zairossani M. Nor ◽  
Melih I. Tamer ◽  
Jeno M. Scharer ◽  
Murray Moo-Young ◽  
Eric J. Jervis
Keyword(s):  
Growth Rate ◽  
Specific Growth Rate ◽  
Batch Culture ◽  
Specific Growth ◽  
Fed Batch ◽  
Kluyveromyces Fragilis ◽  
On Line ◽  
Novel Method ◽  
Fed Batch Culture
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