Extracellular calmodulin acceleratesrbcS-GUS expression in suspension-cultured transgenic tobacco cell

2000 ◽  
Vol 45 (22) ◽  
pp. 2089-2092 ◽  
Author(s):  
Ligeng Ma ◽  
Junli Zhou ◽  
Suqiao Zhang ◽  
Qiang Liu ◽  
Daye Sun
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Tobacco Cell ◽  
Extracellular Calmodulin

scholarly journals Characteristics of the promoters derived from the single-stranded DNA components of Milk vetch dwarf virus in transgenic tobacco

2005 ◽  
Vol 86 (6) ◽  
pp. 1851-1860 ◽  
Author(s):  
Naomi Shirasawa-Seo ◽  
Yoshitaka Sano ◽  
Shigeo Nakamura ◽  
Taka Murakami ◽  
Shigemi Seo ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Types ◽  
Gus Expression ◽  
Cauliflower Mosaic Virus ◽  
Dwarf Virus ◽  
Promoter Regions ◽  
Meristematic Tissue ◽  
Link Protein ◽  
Gus Reporter ◽  
Milk Vetch Dwarf Virus

Predicted promoter regions of Milk vetch dwarf virus (MDV) components (C1–C11) were isolated and fused with a β-glucuronidase (GUS) reporter gene and the characteristics of the promoters were examined. In transgenic tobacco calli, promoters of MDV C4 (encoding a cell-cycle link protein), C5 and C7 (both encoding unknown proteins), C6 (encoding a nuclear-shuttle protein) and C8 (encoding a movement protein) generated a stronger level of GUS expression than the Cauliflower mosaic virus 35S RNA promoter (P35S). In leaves of transgenic tobacco plants, the promoters of C5 and C8 conferred a level of GUS activity comparable to that of P35S. Histochemical GUS analysis showed that the promoters of C4–C9, the latter encoding a capsid protein, were active in phloem and meristematic tissue. The promoter of C8 was also active in mesophyll and cortex cell types. A low level of activity was found for the promoters of C11, which encodes a master replication-initiator protein (Rep), and C1, C2, C3 and C10, which encode additional Reps, in both transgenic tobacco calli and plants.

scholarly journals Histochemical GUS expression of beta tubulin promoter in transgenic tobacco

2017 ◽  
Vol 16 (17) ◽  
pp. 945-952
Author(s):  
Mubeen Hira ◽  
Bashir Aftab ◽  
Ameen Ayesha ◽  
Masood Ammara ◽  
Raza Shahid
Keyword(s):  
Transgenic Tobacco ◽  
Gus Expression ◽  
Beta Tubulin

Biotransformation of hyoscyamine into scopolamine in transgenic tobacco cell cultures

2007 ◽  
Vol 164 (4) ◽  
pp. 521-524 ◽  
Author(s):  
Elisabeth Moyano ◽  
Javier Palazón ◽  
Mercedes Bonfill ◽  
Lidia Osuna ◽  
Rosa M. Cusidó ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Tobacco Cell

Biotransformation of Metamitron by Human P450 Expressed in Transgenic Tobacco Cell Cultures

2006 ◽  
Vol 41 (3) ◽  
pp. 201-222 ◽  
Author(s):  
MAREN BODE ◽  
MATTHIAS HAAS ◽  
TANYA FAYMONVILLE ◽  
BRIGITTE THIEDE ◽  
INGOLF SCHUPHAN ◽  
...  
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Tobacco Cell

Bioreactor strategies for improving production yield and functionality of a recombinant human protein in transgenic tobacco cell cultures

2009 ◽  
Vol 102 (2) ◽  
pp. 508-520 ◽  
Author(s):  
Ting-Kuo Huang ◽  
Michael A. Plesha ◽  
Bryce W. Falk ◽  
Abhaya M. Dandekar ◽  
Karen A. McDonald
Keyword(s):  
Transgenic Tobacco ◽  
Cell Cultures ◽  
Human Protein ◽  
Production Yield ◽  
Tobacco Cell ◽  
Recombinant Human Protein

scholarly journals Expression Pattern of Arabidopsis Thaliana Pollen- and Embryo-Specific Promoter in Transgenic Tobacco Plants

2014 ◽  
Vol 56 (1) ◽  
pp. 73-79 ◽  
Author(s):  
Martin Jopcik ◽  
Ildiko Matusikova ◽  
Jana Moravcikova ◽  
Jana Libantova
Keyword(s):  
Arabidopsis Thaliana ◽  
Transgenic Tobacco ◽  
Expression Profiles ◽  
Pollen Grains ◽  
Apical Part ◽  
Gus Expression ◽  
Transgenic Tobacco Plants ◽  
Specific Expression ◽  
Tobacco Plants ◽  
Biotechnology Research

Abstract Current biotechnology research is focused on tissue-specific expression of genes of interest in plants. Promoters with specific spatial and temporal expression profiles in targeted organisms are in wide use for this. This study investigated whether the Arabidopsis thaliana seed- and pollen-specific promoter MXL maintains its specificity in transgenic tobacco plants. Histochemical analysis revealed that the MXL fusion promoter drives slightly different GUS expression in that heterologous organism. GUS staining was clearly detected in the bicellular stage of pollen development and later in germinating tobacco pollen grains. Unlike in A. thaliana, where the MXL promoter is active during the whole period of embryo development, in tobacco its activity was restricted to a short temporal and spatial window from late-heart to mid-torpedo stages, mainly in the apical part of the developing embryo. These results point to the need to test the expression profiles of heterologous promoters in targeted species before they are used in particular biotechnological programs.

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