Thidiazuron-induced Shoot Multiplication and Plant Regeneration in Bamboo (Dendrocalamus strictus Nees)

Artemisia annua L. axenic plants were used to induce callogeneisis for production of phenolics and plant regeneration. Up to 95% callogenesis from leaf and stem explants on MS supplemented with 2.0 mg/l NAA or 2,4-D + 0.2 mg/l BAP (MSC1 and MSC2) was observed. Lower callus frequency but with improved embryogenic potential was observed upon subculture on medium with reduced auxin and increased BAP concentration (0.5 mg/l NAA or 2,4-D + 0.5 mg/l BAP) (MSC3 and MSC4). The leafinduced callus on NAA/BAP (MSC3) showed best antioxidant potential. Induced shoot regeneration occurred upon high concentration BAP combined with NAA rather than with 2,4-D (0.25 mg/l NAA or 2,4-D + 1.0 mg/l BAP, MSR 1 and MSR2, respectively). Optimal shoot multiplication and rooting were obtained on 1.0 mg/l BAP and 0.1 mg/l IBA, respectively, followed by acclimatization of regenerants to greenhouse conditions. This work aims at establishing protocol for A. annua preservation and biosynthesis of natural products. Plant Tissue Cult. & Biotech. 30(1): 97-106, 2020 (June)

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PLANT REGENERATION OF KAMCHATIC PLANTAIN (PLANTAGO CAMTSCHATICA LINK) THROUGH CALLUS INDUCTION

Mongolian Journal of Agricultural Sciences ◽

10.5564/mjas.v19i3.734 ◽

2017 ◽

Vol 19 (3) ◽

pp. 41-48

Author(s):

Ay N.V. ◽

Duy M.V. ◽

Baatartsogt O. ◽

Altantsetseg Kh. ◽

Enkhchimeg V.

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Leaf Explants ◽

Stage Iv ◽

Shoot Proliferation ◽

Shoot Multiplication ◽

Development Stage ◽

High Survival ◽

Ms Medium

In vitro seedling offspring of Plantago camtschatica Link was investigated regarding induction of somatic embryogenesis in petiole/leaf explants from shoot tissue and shoot proliferation. The aim of study was to investigate the medium supplemented with suitable concentration of plant growth regulators in order to induce somatic embryogenesis, plant regeneration and shoot multiplication. The results showed that: (i) Petiole/young leaf of immature stem induced the highest ratio of calli induction and compact calli formation on MS medium supplemented with 1 mgL-1 2,4-D and 0.5 mgL-1 BA; (ii) From created calli, somatic embryogenesis could be induced on MS medium supplemented with 1 mgL-1 TDZ or 1 mgL-1 TDZ and 0.5 mgL-1 NAA; (iii) MS medium supplemented with 5-7 mgL-1 BA shown the most effective on shoot development stage; (iv) Rooting of shoot was the best on 1/2 solid MS medium with activated charcoal (2 gL-1), and 0.5-4 mgL-1 NAA; and (v) acclimatization of micropropagated plants could be planted in plastic pots containing a mixture of decayed straw : rice husk ashes, (1:1, v/v), sand : soil (1:1, v/v) or soil, showed a high survival rate and most seedlings grew normally.

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Establishment of a Plant Regeneration and Genetic Transformation System of Panax ginseng C.A. Meyer

HortScience ◽

10.21273/hortsci.31.4.572d ◽

1996 ◽

Vol 31 (4) ◽

pp. 572d-572

Author(s):

Hak-Tae Lim ◽

Haeng-Soon Lee ◽

Tage Eriksson

Keyword(s):

Somatic Embryogenesis ◽

Plant Regeneration ◽

Genetic Transformation ◽

Adventitious Shoots ◽

Shoot Multiplication ◽

Regeneration System ◽

Transformation Protocol ◽

Genetic Transformation System ◽

Regeneration Systems

Plant regeneration of ginseng has been known to be difficult, and there are a few reports on plant regeneration of ginseng via somatic embryogenesis. In vitro flowering has, however, been one of the major drawbacks in these regeneration systems in which BA and GA3 were included in germination and shoot multiplication media. Multiplication of adventitious shoots from a single somatic embryo, abnormal morphology, and vitrified shoots were also observed. All these facts have made successful acclimatization of ginseng plantlets difficult. The purposes of this study were 1) to establish the plant regeneration system via organogenesis, 2) to improve normal plant regeneration via somatic embryogenesis, 3) to improve the efficiency of plant regeneration from protoplast culture, 4) to understand the acclimatization process, 5) to develop effective genetic transformation protocol. Data in relation with all these studies are presented in detail.

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Plant regeneration via protocorm-like body formation and shoot multiplication from seed-derived callus of a maudiae type slipper orchid

Acta Physiologiae Plantarum ◽

10.1007/s11738-008-0158-2 ◽

2008 ◽

Vol 30 (5) ◽

pp. 755-759 ◽

Cited By ~ 25

Author(s):

Pou-Ieng Hong ◽

Jen-Tsung Chen ◽

Wei-Chin Chang

Keyword(s):

Plant Regeneration ◽

Shoot Multiplication ◽

Body Formation ◽

Slipper Orchid

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In vitro shoot multiplication and plant regeneration in four Capsicum species using thidiazuron

Scientia Horticulturae ◽

10.1016/j.scienta.2005.06.010 ◽

2006 ◽

Vol 107 (2) ◽

pp. 117-122 ◽

Cited By ~ 23

Author(s):

Venkataiah Peddaboina ◽

Christopher Thamidala ◽

Subhash Karampuri

Keyword(s):

Plant Regeneration ◽

Shoot Multiplication ◽

Capsicum Species

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Plant Regeneration Via Organogenesis and Somatic Embryogenesis in Verbascum Sinuatum L.

Acta Biologica Cracoviensia s Botanica ◽

10.2478/abcsb-2014-0010 ◽

2014 ◽

Vol 56 (1) ◽

pp. 97-103 ◽

Cited By ~ 3

Author(s):

Roya Karamian ◽

Fatemeh Ghasemlou

Keyword(s):

Acetic Acid ◽

Somatic Embryogenesis ◽

Plant Regeneration ◽

Casein Hydrolysate ◽

Shoot Multiplication ◽

Naphthalene Acetic Acid ◽

Ms Medium ◽

Shoot Buds ◽

Traditional Medicines ◽

Mature Embryos

Abstract The genus Verbascum L. belongs to the family Scrophulariaceae and its members are used as medicinal herbs in traditional medicines worldwide. In this study we achieved plant regeneration in Verbascum sinuatum L. via organogenesis and somatic embryogenesis by culture of mature embryos. Embryogenic and nonembryogenic calli were induced from mature embryos on Murashige and Skoog (MS) medium supplemented with different concentrations of benzyl adenine (BA) and a-naphthalene acetic acid (NAA) (but not for 1.5 and 3 mg l−1 NAA). For multiplication of somatic embryoids and differentiation of shoot buds, yellow and friable embryonic calli were transferred to MS medium containing 30 g/l sucrose, 0.5 mg l−1 charcoal and 0.1 or 1 mg l−1 2,4-dichlorophenoxy acetic acid (2,4-D) or to MS medium containing 60 g l−1 sucrose, 50 mg l−1 casein hydrolysate (CH), 0.5 mg l−1 kinetin (Kin), 5 mg l−1 2,4-D and 0.5 mg l−1 charcoal. Shoot multiplication and plantlet regeneration were achieved by transferring shoot buds to MS medium supplemented with 1 mg l−1 BA or Kin.

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