scholarly journals Tissue Culture Applications of Artemisia annua L. Callus for Indirect Organogenesis and Production Phytochemical

2020 ◽  
Vol 30 (1) ◽  
pp. 97-106
Author(s):  
Ely Zayova ◽  
Trendafil Nedev ◽  
Detelina Petrova ◽  
Miroslava Zhiponova ◽  
Venеta Kapchina ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Plant Tissue ◽  
Artemisia Annua ◽  
Shoot Multiplication ◽  
Stem Explants ◽  
High Concentration ◽  
Indirect Organogenesis ◽  
Embryogenic Potential ◽  
Artemisia Annua L

Artemisia annua L. axenic plants were used to induce callogeneisis for production of phenolics and plant regeneration. Up to 95% callogenesis from leaf and stem explants on MS supplemented with 2.0 mg/l NAA or 2,4-D + 0.2 mg/l BAP (MSC1 and MSC2) was observed. Lower callus frequency but with improved embryogenic potential was observed upon subculture on medium with reduced auxin and increased BAP concentration (0.5 mg/l NAA or 2,4-D + 0.5 mg/l BAP) (MSC3 and MSC4). The leafinduced callus on NAA/BAP (MSC3) showed best antioxidant potential. Induced shoot regeneration occurred upon high concentration BAP combined with NAA rather than with 2,4-D (0.25 mg/l NAA or 2,4-D + 1.0 mg/l BAP, MSR 1 and MSR2, respectively). Optimal shoot multiplication and rooting were obtained on 1.0 mg/l BAP and 0.1 mg/l IBA, respectively, followed by acclimatization of regenerants to greenhouse conditions. This work aims at establishing protocol for A. annua preservation and biosynthesis of natural products. Plant Tissue Cult. & Biotech. 30(1): 97-106, 2020 (June)

scholarly journals IN VITRO SHOOT MULTIPLICATION OF STEVIA REBAUDIANA (BERT) THROUGH PLANT TISSUE CULTURE.

2016 ◽  
Vol 4 (11) ◽  
pp. 2300-2307
Author(s):  
Vibha Bhingradiya ◽  
◽  
Archana Mankad ◽  
Ruby Patel ◽  
Shivangi Mathur ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Plant Tissue Culture ◽  
Plant Tissue ◽  
Stevia Rebaudiana ◽  
Shoot Multiplication

scholarly journals Rapid and Efficient Plant Regeneration from Nodal Explants of Artemisia annua L.

2012 ◽  
Vol 22 (1) ◽  
pp. 33-39 ◽  
Author(s):  
B. Janarthanam ◽  
P. Rashmi ◽  
E. Sumathi
Keyword(s):  
Plant Regeneration ◽  
Growth Response ◽  
Artemisia Annua ◽  
Average Length ◽  
Shoot Elongation ◽  
Nodal Explants ◽  
Average Height ◽  
Farm Yard Manure ◽  
Artemisia Annua L ◽  
Half Strength

An efficient plant regeneration protocol was developed for Artemisia annua, an aromatic medicinal herb. Nodal explants inoculated on MS supplemented with 4.44 ?M BAP showed better growth response and produced 116.2 ± 0.1 micro-shoots of an average length 1.9 ± 0.3 cm after 35 days culture. The cluster of small shootlets were cultured on shoot elongation medium supplemented with 1.44 ?M GA3 and 10% coconut milk (CM) showed shoot elongation up to 4.6 ± 0.7 cm. Roots were induced after transfer to half strength MS supplemented with 2.46 ?M IBA produced 14.3 ± 0.2 roots with an average height of 4.3 ± 0.53 cm after 30 days. The rooted plantlets were transferred for hardening, 80 per cent of plantlets survived and resumed growth in the mixture of soil, vermiculite and farm yard manure (1 :  1 : 1). DOI: http://dx.doi.org/10.3329/ptcb.v22i1.11257 Plant Tissue Cult. & Biotech. 22(1): 33-39, 2012 (June)

scholarly journals Somatic Embryogenesis and Plant Regeneration in Tissue Cultures of Artemisia annua L.

2007 ◽  
Vol 34 (3) ◽  
pp. 197-200 ◽  
Author(s):  
Pil-Son Choi ◽  
Sung-Ran Min ◽  
Suk-Min Ko ◽  
Jang-R. Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Artemisia Annua ◽  
Tissue Cultures ◽  
Artemisia Annua L

scholarly journals Use an Organic Biostimulant (Vermicompost Tea) For Enhancement In Vıtro Callus Growth in Sainfoin (Onobrychis viciifolia Scop.)

2019 ◽  
Vol 7 (2) ◽  
pp. 214
Author(s):  
Ramazan Beyaz ◽  
Fevziye Şüheyda Hepşen Türkay
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Plant Growth ◽  
Physiological Responses ◽  
Callus Growth ◽  
Stem Explants ◽  
Onobrychis Viciifolia ◽  
Callus Initiation ◽  
Vermicompost Tea

The health and vitality of callus growth is one of the prerequisites for the success of further in vitro studies. This study investigated the efficiency of different percentage (0%, 10%, 20%, 30%, and 40%) of vermicompost tea as an organic substance on in vitro callus growth in sainfoin. Morpho-physiological responses of calli to vermicompost tea measured under in vitro conditions. As a result of this investigation, a combination of plant growth regulators (4 mg/l BAP and 0.5 mg/l NAA) with 20% of vermicompost tea causing significant callus initiation and growth in sainfoin stem explants. Under the light of these scientific findings, vermicompost tea might be used as an organic bio stimulant for efficient callus growth and complementary to commercial chemical hormones in sainfoin. This research is important due to it can contribute positively to the plant species that are difficult in terms of callus growth and plant regeneration in tissue culture.

Artemisinin Production by Shoot Regeneration of Artemisia annua L. Using Thidiazuron

2008 ◽  
Vol 63 (1-2) ◽  
pp. 96-100 ◽  
Author(s):  
Wanwimon Lualon ◽  
Wanchai De-Eknamkul ◽  
Hiroyuki Tanaka ◽  
Yukihiro Shoyama ◽  
Waraporn Putalun
Keyword(s):  
Plant Growth ◽  
Plant Growth Regulators ◽  
Growth Regulators ◽  
Artemisia Annua ◽  
Multiple Shoot ◽  
Stem Explants ◽  
Ms Medium ◽  
Artemisia Annua L ◽  
Bud Induction

An efficient in vitro method for multiple shoot bud induction and regeneration has been developed in Artemisia annua L. using leaf and stem explants in various concentrations and combinations of plant growth regulators to evaluate the frequency of regeneration. The sources of explants as well as plant growth regulators in the medium were found to influence the multiple shoot induction. The result shows that the stem segment cultured on Murashige and Skoog (MS) medium supplemented with 0.1 mg/l thidiazuron (TDZ) gave a perfect shoot formation (100%) and good shoot multiplication (57 shoots/explant) after 2 weeks of culture. Healthy regenerated shoots were elongated and rooted in MS medium without hormones. The artemisinin content in plants regenerated from stem explants using 0.1 mg/l TDZ was (3.36 ± 0.36) μg/mg dry weight and two-fold higher than that of in vitro grown plants of the same age [(1.73 ± 0.23) μg/mg DW]. This system exhibited a potential for a rapid propagation of shoots from the stem explant and makes it possible to develop a clonal propagation of A. annua.

scholarly journals The Impact of Carbon Source, Explants and Growth Regulators on Callogenesis and Organogenesis of Artemisia annua

2015 ◽  
Vol 7 (4) ◽  
pp. 475-485 ◽  
Author(s):  
Bita GHASSEMI ◽  
Fatemeh DEHGHAN NAYERI ◽  
Ramin HOSSEINI
Keyword(s):  
Carbon Source ◽  
Artemisia Annua ◽  
Antimalarial Activity ◽  
Leaf Explants ◽  
Basal Medium ◽  
Stem Explants ◽  
Root Explants ◽  
Artemisia Annua L ◽  
High Concentrations ◽  
The Impact

Artemisinin, a sesquiterpene lactone isolated from Artemisia annua L. plant is known for its antimalarial activity. The low content of artemisinin has stimulated researchers to enhance its production through biotechnological approaches such as tissue culture. The present study was initiated to study the effect of some important factors alone and in combination, on the callogenesis and organogenesis of Artemisia annua. The type of carbon source had a significant effect on NAA efficiency to callogenesis of A. annua, whereas the best callogenesis of A. absinthium was observed at 2 mg/L BAP + 30 g/L sucrose and in the absence of NAA, with root explants. Presence of BAP also had an important effect on callogenesis, especially in high concentrations. A suitable suspension culture was obtained in the MS basal medium containing 0.5 mg/L NAA and BAP, with 30 g/L glucose. Artemisinin was naturally production was at least 0.03 mg/g (w.dt) at the first day and peaked on the 16th day with 0.31 mg/g (w.dt) in the cell culture of A. annua. The maximum number of shoots (2.167 ± 1.484) was induced at 0.5 mg/L BAP + 0.1 mg/L NAA + glucose (30 g/L) with leaf explants. However, treatments containing glucose did not show a good shoot induction. Longer shoots were induced in the medium containing either 0.5 mg/l NAA + 0.5 mg/l BAP + sucrose with leaf explants (1.493 cm ± 0.342) or 0.5 mg/l NAA + sucrose with stem explants (0.697 cm ± 0.930). Medium containing 0.5 or 2 mg/l NAA and sucrose (without BAP) induced more roots though.

REVIEW: In vitro plant regeneration studies and their potential applications in Populus spp.: a review

2016 ◽  
Vol 63 (2) ◽  
pp. 77-84 ◽  
Author(s):  
Ayesh Gaur ◽  
Pankaj Kumar ◽  
Ajay Kumar Thakur ◽  
Dinesh Kumar Srivastava
Keyword(s):  
Tissue Culture ◽  
Plant Regeneration ◽  
Large Scale ◽  
Direct Organogenesis ◽  
Attractive Alternative ◽  
Special Role ◽  
Scale Production ◽  
Multiplication Rate ◽  
Indirect Organogenesis

Genus Populus comprises about 25–35 species of deciduous flowering plants in the family Salicaceae which are widely distributed in temperate climates of the Northern Hemisphere. Populus species are important resources in certain branches of industry and have a special role for the scientific study of biological and agricultural systems. The poplar is known for its remarkable significance among the commercially propagated tree species such as teak, eucalyptus, wild cherry, red wood, and radiata pine. In vitro regeneration refers to growing and multiplications of cells, tissues and organs on defined liquid/solid media under aseptic and controlled environments. In vitro clonal propagation of forest trees, due to the high multiplication rate, is an attractive alternative for rapid propagation of elite genotypes of those species that could not easily be propagated through conventional methods. Owing to their widespread uses at the industrial level and for meeting the ever-increasing global demand for biomass production and wood industry, tissue culture techniques can be exploited for rapid cloning and large-scale production of planting material of various poplar species. Recent progress in the field of plant tissue culture determined this area to be one of the most dynamic and promising for experimental biology. Much work has been carried out on in vitro plant regeneration studies in Populus spp. including direct organogenesis, indirect organogenesis and somatic embryogenesis. These reviews provide an insight for in vitro plant regeneration studies in poplar species and their potential in its improvement.

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