Plantlet Regeneration via Multiple Shoot Induction in Indian Cultivars of Lucerne (Medicago sativa L)

2008 ◽  
Vol 17 (2) ◽  
pp. 181-184 ◽  
Author(s):  
Suresh Kumar ◽  
Amaresh Chandra ◽  
Madan G. Gupta
Keyword(s):  
Medicago Sativa ◽  
Multiple Shoot ◽  
Plantlet Regeneration ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Medicago Sativa L

Mutagen treatment affects the regeneration efficiency of the shoot-tip explant in Celosia cristate L. (Cockscomb)

2020 ◽  
Vol 80 (03) ◽  
Author(s):  
Rupesh S. Badere ◽  
Pallavi K. Rinkey
Keyword(s):  
Plant Growth Regulator ◽  
Gamma Ray ◽  
Multiple Shoot ◽  
Shoot Tip ◽  
Ms Medium ◽  
Shoot Induction ◽  
Ethyl Methane Sulphonate ◽  
Multiple Shoot Induction ◽  
Methane Sulphonate ◽  
Regeneration Efficiency

The shoot-tip explant harvested from ethyl methane sulphonate (EMS) and gamma ray (GR) mutagenized seedling was cultured over MS medium fortified with NAA and BAP for five generations to amplify the mutated sector. Mutagens reduced the regeneration efficiency of the explant and affected its plant growth regulator-dependence for multiple shoot induction. While the 12d-old shoot-tip from GR-treated seedling induced shoots with 0.5µM NAA+6.6µM BAP; that from EMS-treated seedling induced shoots with 8.8µM BAP. The present study establishes that the mutagens affect the regeneration process in the explant.

scholarly journals In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

2010 ◽  
Vol 20 (1) ◽  
pp. 73-79 ◽  
Author(s):  
M. F. Hasan ◽  
B. Sikdar
Keyword(s):  
Plant Regeneration ◽  
In Vitro Propagation ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Shoot Tips ◽  
Root Induction ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Polygonum Hydropiper

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully. Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation D.O.I. 10.3329/ptcb.v20i1.5970 Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

scholarly journals Direct multiple shoot induction and plant regeneration from dormant buds of Codonopsis pilosula (Franch.) Nannf.

2011 ◽  
Vol 10 (51) ◽  
pp. 10509-10515 ◽  
Author(s):  
H Zhang Y ◽  
F Gao S ◽  
Du T ◽  
G Chen H ◽  
H Z Wang ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Multiple Shoot ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Codonopsis Pilosula

RAPID MASS PROPAGATION THROUGH MULTIPLE SHOOT INDUCTION FROM RHIZOME OF CHINESE CYMBIDIUMS

2010 ◽  
pp. 213-217 ◽  
Author(s):  
H.-F. Chiang ◽  
J.-R. Lin ◽  
C.-Y. Kao ◽  
K.-S. Liu ◽  
Y. Chu
Keyword(s):  
Multiple Shoot ◽  
Shoot Induction ◽  
Mass Propagation ◽  
Multiple Shoot Induction ◽  
Rapid Mass

scholarly journals STANDARDIZATION OF MEDIUM FOR SYNTHETIC SEED GERMINATION OF SALVIA SCLAREA L.

2015 ◽  
Vol 2 (1) ◽  
pp. 118-120
Author(s):  
Durgha H ◽  
Ramya G ◽  
Gogul Ramanth M ◽  
Thirugnanasampandan R
Keyword(s):  
Seed Germination ◽  
Sodium Alginate ◽  
Calcium Chloride ◽  
Multiple Shoot ◽  
Nodal Explants ◽  
Synthetic Seed ◽  
Ms Medium ◽  
Shoot Induction ◽  
Multiple Shoot Induction ◽  
Salvia Sclarea

Young nodal explants (0.5-1cm) of Salvia sclarea L. was used for synthetic seed preparation.Synthetic seeds were prepared using 5% sodium alginate and 1.11% calcium chloride. Seed germination was observed on MS medium fortified with 1.4µM GA3+4.4µM BA after twenty days of culture. Further multiple shoot induction was observed after fifteen days of shootinduction.

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