Fed-batch culture of yeast Saccharomyces cerevisiae with a DO-stat method by a fuzzy controller

1998 ◽  
Vol 18 (2) ◽  
pp. 79-82 ◽  
Author(s):  
Fan-Chiang Yang ◽  
Der-Wei Maa
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Batch Culture ◽  
Fuzzy Controller ◽  
Fed Batch ◽  
Yeast Saccharomyces Cerevisiae ◽  
Fed Batch Culture ◽  
Stat Method

scholarly journals A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

2021 ◽  
Author(s):  
Massimo Sanchez ◽  
Emanuela Palomba ◽  
Valentina Tirelli ◽  
Elisabetta de Alteriis ◽  
Carmine Landi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Batch Culture ◽  
Time Course ◽  
Reference Model ◽  
Product Yield ◽  
Fed Batch ◽  
Yeast Saccharomyces Cerevisiae ◽  
Biotechnological Processes ◽  
Fed Batch Culture

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

Application of genetic algorithm to self-organizing fuzzy controller in fed-batch culture ofScutellaria baicalensis G. plant cell

1998 ◽  
Vol 15 (4) ◽  
pp. 404-410 ◽  
Author(s):  
Jeong-Woo Choi ◽  
Jin Man Cho ◽  
Jung Gun Lee ◽  
Won Hong Lee ◽  
Ik Hwan Kim ◽  
...  
Keyword(s):  
Genetic Algorithm ◽  
Batch Culture ◽  
Plant Cell ◽  
Fuzzy Controller ◽  
Fed Batch ◽  
Fed Batch Culture ◽  
Self Organizing

Fed-batch culture with a modified DO-stat method

1991 ◽  
Vol 71 (1) ◽  
pp. 35-38 ◽  
Author(s):  
Takuo Yano ◽  
Takahiko Endo ◽  
Takashi Tuji ◽  
Yoshinori Nishizawa
Keyword(s):  
Batch Culture ◽  
Fed Batch ◽  
Fed Batch Culture ◽  
Stat Method

scholarly journals A cytofluorimetric analysis of a Saccharomyces cerevisiae population cultured in a fed-batch bioreactor

PLoS ONE ◽  
2021 ◽  
Vol 16 (6) ◽  
pp. e0248382
Author(s):  
Emanuela Palomba ◽  
Valentina Tirelli ◽  
Elisabetta de Alteriis ◽  
Palma Parascandola ◽  
Carmine Landi ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Cell Cycle ◽  
Batch Culture ◽  
Time Course ◽  
Reference Model ◽  
Product Yield ◽  
Fed Batch ◽  
Batch Bioreactor ◽  
Biotechnological Processes ◽  
Fed Batch Culture

The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses.

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