Enhanced production of the alkaloid nicotine in hairy root cultures of Nicotiana tabacum L.

2012 ◽  
Vol 113 (1) ◽  
pp. 121-129 ◽  
Author(s):  
Bo Zhao ◽  
Foster A. Agblevor ◽  
Ritesh K. C. ◽  
John G. Jelesko
Keyword(s):  
Nicotiana Tabacum ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production ◽  
Nicotiana Tabacum L

Enhanced production of falcarinol-type polyacetylenes in hairy roots of cultivated carrot (Daucus carota subsp. sativus)

2021 ◽  
Author(s):  
Frank Dunemann ◽  
Christoph Böttcher
Keyword(s):  
Hairy Root ◽  
Large Scale ◽  
Higher Plants ◽  
Leaf Tissue ◽  
Hairy Root Cultures ◽  
Scale Production ◽  
Root Cultures ◽  
Tissue Samples ◽  
Positive Effects ◽  
Enhanced Production

Abstract Polyacetylenes (PAs) are a large group of bioactive phytochemicals, which are primarily produced by higher plants of the families Apiaceae and Araliaceae. Especially aliphatic C17-polyacetylenes of the falcarinol-type such as falcarinol (FaOH) and falcarindiol (FaDOH) are known for their numerous positive effects on human health. In this study we investigate the potential of carrot hairy root cultures for production of PAs. Three individual plants of seven differently coloured carrot cultivars were used for the development of hairy root cultures by transformation of root discs with the wild-type Rhizobium rhizogenes strain 15834. A total of 51 individual hairy root (HR) lines were obtained and quantitatively analysed together with root, petiole and leaf tissue samples for FaOH and FaDOH. Among the five tissues sampled from the donor plants, root periderm samples generally exhibited the highest PA levels with FaDOH as prevailing PA and large differences between cultivars. In comparison to periderm tissue, FaOH levels were highly increased in HR lines of all cultivars. In contrast, FaDOH levels were not significantly altered. Considering the low to moderate PA concentration in root and leaf tissues of the orange cultivars there was an up to more than 10-fold increase of the FaOH concentration in HRs of these genotypes. Within this study a reproducible method for Rrhi-mediated transformation of carrot root discs was applied which provides an efficient tool to assess the function of candidate genes involved in the biosynthesis of key PAs in carrot but might be used in future also for the large-scale production of falcarinol-type PAs.

Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 203-208 ◽  
Author(s):  
Suchitra Banerjee ◽  
L Rahman ◽  
G.C Uniyal ◽  
P.S Ahuja
Keyword(s):  
Agrobacterium Rhizogenes ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production

Induction of Pyridine Alkaloid Formation in Transformed Root Cultures of Nicotiana tabacum

2009 ◽  
Vol 64 (11-12) ◽  
pp. 869-874 ◽  
Author(s):  
Rawia Zayed ◽  
Michael Wink
Keyword(s):  
Salicylic Acid ◽  
Nicotiana Tabacum ◽  
Hairy Root ◽  
Inhibitory Effect ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Fresh Weight ◽  
Root Tips ◽  
Transformed Root Cultures ◽  
Set Up

Hairy root cultures of Nicotiana tabacum were set up by excised root tips with Agrobacterium rhizogenes. The successful transformation was confirmed by analyzing rolC and virC genes using polymerase chain reaction (PCR). Hairy root cultures were employed to study the formation of pyridine alkaloids, mainly nicotine. The transformed cultures were incubated with potential elicitors, such as methyljasmonate, quercetin and salicylic acid, in order to stimulate the biosynthesis of pyridine alkaloids. Profile and amounts of pyridine alkaloids were analyzed using capillary GLC-MS. Treatment of the cultures with methyljasmonate (50 μM) increased the alkaloid accumulation ca. 7-fold up to a level of 0.58 mg/g fresh weight as compared to untreated controls. Quercetin (200 μM) enhanced the alkaloid production ca. 4-fold (0.34 mg/g fresh weight) within 24 h. In contrast salicylic acid in all tested concentrations decreased the alkaloid level to 1 μg/g fresh weight. Also the inhibitory effect of salicylic acid on the elicitation effect of methyljasmonate and quercetin was investigated.

Enhanced production of artemisinin by Artemisia annua L hairy root cultures in a modified inner-loop airlift bioreactor

1998 ◽  
Vol 19 (5) ◽  
pp. 389-392 ◽  
Author(s):  
C. Liu ◽  
Y. Wang ◽  
C. Guo ◽  
F. Ouyang ◽  
H. Ye ◽  
...  
Keyword(s):  
Hairy Root ◽  
Artemisia Annua ◽  
Airlift Bioreactor ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Artemisia Annua L ◽  
Enhanced Production

Enhanced Production of Resveratrol, Piceatannol, Arachidin-1, and Arachidin-3 in Hairy Root Cultures of Peanut Co-treated with Methyl Jasmonate and Cyclodextrin

2015 ◽  
Vol 63 (15) ◽  
pp. 3942-3950 ◽  
Author(s):  
Tianhong Yang ◽  
Lingling Fang ◽  
Cesar Nopo-Olazabal ◽  
Jose Condori ◽  
Luis Nopo-Olazabal ◽  
...  
Keyword(s):  
Methyl Jasmonate ◽  
Hairy Root ◽  
Hairy Root Cultures ◽  
Root Cultures ◽  
Enhanced Production
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