scholarly journals A structured growth model for Cynara cardunculus cell suspension

2000 ◽  
Vol 23 (2) ◽  
pp. 199-203 ◽  
Author(s):  
P. M. N. A. C. Pires Cabral ◽  
M. E. Lima Costa ◽  
J. M. S. Cabral
Keyword(s):  
Cell Suspension ◽  
Growth Model ◽  
Cynara Cardunculus

Proteases from cell suspension cultures of Cynara cardunculus

1993 ◽  
Vol 33 (6) ◽  
pp. 1323-1326 ◽  
Author(s):  
Maria Cordeiro ◽  
Zhong-Tian Xue ◽  
M. Salomé Pais ◽  
Peter E. Brodelius
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cynara Cardunculus

Protease and phenol production of Cynara cardunculus L. cell suspension in a chemostat

1996 ◽  
Vol 19 (7) ◽  
pp. 493-500 ◽  
Author(s):  
M.E. Lima Costa ◽  
W.M. van Gulik ◽  
H.J.G. ten Hoopen ◽  
M.S.S. Pais ◽  
J.M.S. Cabral
Keyword(s):  
Cell Suspension ◽  
Cynara Cardunculus ◽  
L Cell

Electron microscopy of keratinocytes cultured on a collagen substrate used as an allograft for the treatment of chronic wounds

1992 ◽  
Vol 50 (2) ◽  
pp. 1104-1105
Author(s):  
Debby A. Jennings ◽  
Michael J. Morykwas ◽  
Louis C. Argenta
Keyword(s):  
Electron Microscopy ◽  
Cell Suspension ◽  
Single Cell ◽  
Chronic Wounds ◽  
Mechanical Stability ◽  
Uv Light ◽  
Type I ◽  
Single Cell Suspension ◽  
Collagen Substrate ◽  
Dermal Collagen

Grafts of cultured allogenic or autogenic keratlnocytes have proven to be an effective treatment of chronic wounds and burns. This study utilized a collagen substrate for keratinocyte and fibroblast attachment. The substrate provided mechanical stability and augmented graft manipulation onto the wound bed. Graft integrity was confirmed by light and transmission electron microscopy.Bovine Type I dermal collagen sheets (100 μm thick) were crosslinked with 254 nm UV light (13.5 Joules/cm2) to improve mechanical properties and reduce degradation. A single cell suspension of third passage neonatal foreskin fibroblasts were plated onto the collagen. Five days later, a single cell suspension of first passage neonatal foreskin keratinocytes were plated on the opposite side of the collagen. The grafts were cultured for one month.The grafts were fixed in phosphate buffered 4% formaldehyde/1% glutaraldehyde for 24 hours. Graft pieces were then washed in 0.13 M phosphate buffer, post-fixed in 1% osmium tetroxide, dehydrated, and embedded in Polybed 812.

Three-dimensional distribution of ribulose-1, 5-bisphosphate carboxylase/oxygemase during the early development of proplastids in dark-grown Euglena cells transferred to an inorganic medium

1990 ◽  
Vol 48 (3) ◽  
pp. 906-907
Author(s):  
Tomoko Ehara ◽  
Shuji Sumida ◽  
Tetsuaki Osafune ◽  
Eiji Hase
Keyword(s):  
Cell Suspension ◽  
Euglena Gracilis ◽  
Carbon Materials ◽  
Three Dimensional ◽  
Peripheral Region ◽  
Plastid Development ◽  
Bisphosphate Carboxylase ◽  
Inorganic Medium ◽  
Ribulose 1 ◽  
Dimensional Distribution

As shown previously, Euglena cells grown in Hutner’s medium in the dark without agitation accumulate wax as well as paramylum, and contain proplastids showing no internal structure except for a single prothylakoid existing close to the envelope. When the cells are transferred to an inorganic medium containing ammonium salt and the cell suspension is aerated in the dark, the wax was oxidatively metabolized, providing carbon materials and energy 23 for some dark processes of plastid development. Under these conditions, pyrenoid-like structures (called “pro-pyrenoids”) are formed at the sites adjacent to the prolamel larbodies (PLB) localized in the peripheral region of the proplastid. The single prothylakoid becomes paired with a newly formed prothylakoid, and a part of the paired prothylakoids is extended, with foldings, in to the “propyrenoid”. In this study, we observed a concentration of RuBisCO in the “propyrenoid” of Euglena gracilis strain Z using immunoelectron microscopy.

Implications of fault array evolution for synrift depocentre development: insights from a numerical fault growth model

2000 ◽  
Vol 12 (3-4) ◽  
pp. 241-261 ◽  
Author(s):  
P. A. Cowie ◽  
S. Gupta ◽  
N. H. Dawers
Keyword(s):  
Growth Model ◽  
Fault Growth

Transformation of Papaver somniferum cell suspension cultures with sam1 from A. thaliana results in cell lines of different S-adenosyl-L-methionine synthetase activity

1997 ◽  
Vol 99 (2) ◽  
pp. 233-240 ◽  
Author(s):  
Muriel Belny ◽  
Didier Herouart ◽  
Brigitte Thomasset ◽  
Helene David ◽  
Annie Jacquin-Dubreuil ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Lines ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Papaver Somniferum ◽  
Synthetase Activity
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