UCP1 and AOX1a contribute to regulation of carbon and nitrogen metabolism and yield in Arabidopsis under low nitrogen stress

2022 ◽  
Author(s):  
Xinyan Qiao ◽  
Mengjiao Ruan ◽  
Tao Yu ◽  
Chaiyan Cui ◽  
Cuiyun Chen ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Stress ◽  
Carbon And Nitrogen ◽  
Low Nitrogen Stress ◽  
Carbon And Nitrogen Metabolism ◽  
Low Nitrogen

scholarly journals Transcriptomic and Metabolomics Analysis of Different Endosperm Region under Nitrogen Treatments

2019 ◽  
Vol 20 (17) ◽  
pp. 4212 ◽  
Author(s):  
Dongyun Ma ◽  
Honghuan Gao ◽  
Chenyang Du ◽  
Lingli Li ◽  
Wan Sun ◽  
...  
Keyword(s):  
Transcription Factors ◽  
Nitrogen Metabolism ◽  
Protein Distribution ◽  
Post Translational Modification ◽  
Expression Change ◽  
Heterogeneous Distribution ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism ◽  
Nitrogen Treatments ◽  
Low Nitrogen

Storage protein distribution in wheat-grain endosperm is heterogeneous, but the underlying molecular mechanism remains unclear. Two parts of the endosperm region, the innermost endosperm (IE) region and the remaining endosperm (RE) region, grown under low nitrogen (LN) and high nitrogen (HN) treatments were used to perform metabolomic and transcriptomic analysis. We identified 533 and 503 differentially expressed genes (DEGs) with at least a two-fold expression change (p < 0.05) between IE and RE, among which 81 and 78 transcripts under LN and HN, respectively, related to carbon and nitrogen metabolism, and encoded transcription factors or proteins involved in post-translational modification (PTM). The significantly differentially abundant metabolites between IE and RE were mainly amino acids, N-compounds, carbohydrates, and nucleic acids. More upregulated transcripts and metabolites were identified in RE than IE under HN conditions, indicating that HN activates metabolism in the endosperm periphery. In addition to carbon and nitrogen metabolism, transcription factors and protein PTMs, such as phosphorylation and acetylation, might determine the protein heterogeneous distribution between IE and RE and its response to nitrogen fertilizer supply.

scholarly journals An Integrated Analysis of the Rice Transcriptome and Metabolome Reveals Differential Regulation of Carbon and Nitrogen Metabolism in Response to Nitrogen Availability

2019 ◽  
Vol 20 (9) ◽  
pp. 2349 ◽  
Author(s):  
Wei Xin ◽  
Lina Zhang ◽  
Wenzhong Zhang ◽  
Jiping Gao ◽  
Jun Yi ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Nitrogen Use Efficiency ◽  
Nitrogen Availability ◽  
Tca Cycle ◽  
Integrated Analysis ◽  
Nitrogen Use ◽  
Carbon And Nitrogen ◽  
Use Efficiency ◽  
Carbon And Nitrogen Metabolism ◽  
Low Nitrogen

Nitrogen (N) is an extremely important macronutrient for plant growth and development. It is the main limiting factor in most agricultural production. However, it is well known that the nitrogen use efficiency (NUE) of rice gradually decreases with the increase of the nitrogen application rate. In order to clarify the underlying metabolic and molecular mechanisms of this phenomenon, we performed an integrated analysis of the rice transcriptome and metabolome. Both differentially expressed genes (DEGs) and metabolite Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis indicated that carbon and nitrogen metabolism is significantly affected by nitrogen availability. Further analysis of carbon and nitrogen metabolism changes in rice under different nitrogen availability showed that high N inhibits nitrogen assimilation and aromatic metabolism pathways by regulating carbon metabolism pathways such as the tricarboxylic acid (TCA) cycle and the pentose phosphate pathway (PPP). Under low nitrogen, the TCA cycle is promoted to produce more energy and α-ketoglutarate, thereby enhancing nitrogen transport and assimilation. PPP is also inhibited by low N, which may be consistent with the lower NADPH demand under low nitrogen. Additionally, we performed a co-expression network analysis of genes and metabolites related to carbon and nitrogen metabolism. In total, 15 genes were identified as hub genes. In summary, this study reveals the influence of nitrogen levels on the regulation mechanisms for carbon and nitrogen metabolism in rice and provides new insights into coordinating carbon and nitrogen metabolism and improving nitrogen use efficiency in rice.

scholarly journals Expression Analysis of Nitrogen Metabolism-Related Genes Reveals Differences in Adaptation to Low-Nitrogen Stress between Two Different Barley Cultivars at Seedling Stage

2018 ◽  
Vol 2018 ◽  
pp. 1-10 ◽  
Author(s):  
Zhiwei Chen ◽  
Chenghong Liu ◽  
Yifei Wang ◽  
Ting He ◽  
Runhong Gao ◽  
...  
Keyword(s):  
Gene Expression ◽  
Nitrogen Metabolism ◽  
Expression Analysis ◽  
Nitrogen Fertilizers ◽  
Nitrogen Stress ◽  
Environmental Damage ◽  
Barley Cultivars ◽  
Low Nitrogen Stress ◽  
Low Nitrogen Tolerance ◽  
Low Nitrogen

The excess use of nitrogen fertilizers causes many problems, including higher costs of crop production, lower nitrogen use efficiency, and environmental damage. Crop breeding for low-nitrogen tolerance, especially molecular breeding, has become the major route to solving these issues. Therefore, in crops such as barley (Hordeum vulgare L.), it is crucial to understand the mechanisms of low-nitrogen tolerance at the molecule level. In the present study, two barley cultivars, BI-04 (tolerant to low nitrogen) and BI-45 (sensitive to low nitrogen), were used for gene expression analysis under low-nitrogen stress, including 10 genes related to primary nitrogen metabolism. The results showed that the expressions of HvNIA2 (nitrite reductase), HvGS2 (chloroplastic glutamine synthetase), and HvGLU2 (ferredoxin-dependent glutamate synthase) were only induced in shoots of BI-04 under low-nitrogen stress, HvGLU2 was also only induced in roots of BI-04, and HvGS2 showed a rapid response to low-nitrogen stress in the roots of BI-04. The expression of HvASN1 (asparagine synthetase) was reduced in both cultivars, but it showed a lower reduction in the shoots of BI-04. In addition, gene expression and regulation differences in the shoots and roots were also compared between the barley cultivars. Taken together, the results indicated that the four above-mentioned genes might play important roles in low-nitrogen tolerance in barley.

Low nitrogen level improves low-light tolerance in tall fescue by regulating carbon and nitrogen metabolism

2022 ◽  
Vol 194 ◽  
pp. 104749
Author(s):  
Suyun Wen ◽  
Bowen Liu ◽  
Si Long ◽  
Shuanghong Gao ◽  
Qian Liu ◽  
...  
Keyword(s):  
Nitrogen Metabolism ◽  
Tall Fescue ◽  
Nitrogen Level ◽  
Low Light ◽  
Carbon And Nitrogen ◽  
Carbon And Nitrogen Metabolism ◽  
Low Nitrogen

scholarly journals Activities of carbon and nitrogen metabolism enzymes during germinating sorghum seeds and early seedlings growth

2017 ◽  
Vol 45 (4) ◽  
pp. 587-597
Author(s):  
R. Ben Mrid ◽  
R. El Omari ◽  
Y. Bouargalne ◽  
N. El Mourabit ◽  
M. Nhiri
Keyword(s):  
Nitrogen Metabolism ◽  
Carbon And Nitrogen ◽  
Metabolism Enzymes ◽  
Carbon And Nitrogen Metabolism
Sign in / Sign up

Export Citation Format

Share Document