Pneumonie, Zweiklappenendokarditis und Lungenarterienembolie bei einem 26-jährigen Patienten

Der Internist ◽

10.1007/s00108-021-01228-1 ◽

2021 ◽

Author(s):

Lisa Hasselbach ◽

Maximilian Dübbers

Keyword(s):

Coxiella Burnetii ◽

Ross Operation

ZusammenfassungEin 26-jähriger Patient zeigte eine linksseitige Pneumonie, eine Zweiklappenendokarditis und eine Lungenarterienembolie. Im Jahr 2004 war er bei angeborener Aortenklappenstenose mittels Ross-Operation versorgt worden. Es zeigte sich ein Anstieg der Titer für Coxiella burnetii, den Erreger des Q‑Fiebers. Unter antibiotischer Therapie mit Levofloxacin und Doxycyclin kam es zu einer Besserung der Symptomatik und einem Rückgang der Entzündungsparameter. Die Therapie der Endokarditis war erfolgreich.

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A comparison of three cryotechniques (freeze-drying, cryosubstitution, cryosectioning) of specimen preparation for immunolabelling of bacterial antigens of Coxiella burnetii

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100168438 ◽

1994 ◽

Vol 52 ◽

pp. 140-141

Author(s):

T. F. McCaul ◽

R. J. Gould

Keyword(s):

Coxiella Burnetii ◽

Freeze Drying ◽

Stress Proteins ◽

Cultured Cells ◽

High Specificity ◽

Specimen Preparation ◽

Bacterial Antigens ◽

Structural Preservation ◽

Nuclear Ribonucleoprotein ◽

Cellular Components

Immuno-electron microscopy has allowed the selective localisation of molecules with high resolution and high specificity. Cryopreparatory methods have provided better retention of antigenicity suitable for precise immunolabelling together with optimal structural preservation of cellular components. Cryosubstitution and cryoultramicrotomy have widely been exploited for immunolabelling. Molecular Distillation Dryer (MDD), a form of freeze-drying technique, has recently been used for immunolabelling of Plasmodium falciparum stress proteins and nuclear ribonucleoprotein particles in cultured cells. In the present study, we report the comparison of all three cryotechniques in the immunolabelling of bacterial antigens of Coxiella burnetii.The highly infectious C. burnetii was prefixed in 3% glutaraldehyde (66 mM cacodylate buffer, pH 6.8 ). The cells were then pre-embedded in 2% low-temperature agarose on Durapore hydrophilic membrane prior to cryofixation using a LifeCell CF100 metal-mirror system. A 1% glutaraldehyde in 100% methanol was used as a medium for cryosubstitution in a Reichert CS Auto Cryosubstitution apparatus.

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