Cow fever – High seroprevalence of Coxiella burnetii antibodies in veterinarians associated with obstetric activity on cattle, Germany, 2009

Abstract A cross-sectional study was carried out to determine the seroprevalence and risk factors of Q fever in sheep in the northern part of Kaduna State, Nigeria. This study aimed to determine Coxiella burnetii infection and its risk factors in sheep in Kaduna State. A total of 400 blood samples consisting of 259 samples from females and 141 from males were aseptically collected from the jugular vein of sheep from flocks in Kaduna State. The sera obtained were screened for Q fever using an indirect enzyme-linked immunosorbent assay (iELISA). The obtained data were analysed to determine whether there is a relationship between sex, age, and the animals tested. The analysis revealed that 8.0% of the sera was seropositive by iELISA. There was no significant difference in Q fever seropositivity in the study area according to the sex of sheep (P > 0.05). There was a statistically significant difference (P < 0.05) in Q fever seropositivity according to the age of sheep. This study indicated a high seroprevalence of Q fever mainly among female animals and older sheep. Further studies are required to determine the epizootiology of Q fever in the study area more precisely.

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High seroprevalence of Coxiella burnetii in dairy cattle in China

Tropical Animal Health and Production ◽

10.1007/s11250-015-0968-3 ◽

2015 ◽

Vol 48 (2) ◽

pp. 423-426 ◽

Cited By ~ 4

Author(s):

Heba S. El-Mahallawy ◽

Patrick Kelly ◽

Jilei Zhang ◽

Yi Yang ◽

Hui Zhang ◽

...

Keyword(s):

Dairy Cattle ◽

Coxiella Burnetii ◽

High Seroprevalence

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High seroprevalence of Coxiella burnetii infection in Eastern Cantabria (Spain)

International Journal of Epidemiology ◽

10.1093/ije/27.1.142 ◽

1998 ◽

Vol 27 (1) ◽

pp. 142-145 ◽

Cited By ~ 22

Author(s):

F. Pascual-Velasco ◽

M. Montes ◽

J. Marimon ◽

G. Cilla

Keyword(s):

Coxiella Burnetii ◽

High Seroprevalence

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Q fever (Coxiella burnetii) reservoir in wild brown rat (Rattus norvegicus) populations in the UK

Parasitology ◽

10.1017/s0031182000081014 ◽

1995 ◽

Vol 110 (1) ◽

pp. 31-35 ◽

Cited By ~ 52

Author(s):

J. P. Webster ◽

G. Lloyd ◽

D. W. Macdonald

Keyword(s):

Coxiella Burnetii ◽

Rattus Norvegicus ◽

Q Fever ◽

Causal Agent ◽

Captive Population ◽

High Seroprevalence ◽

Brown Rat ◽

Wild Rats ◽

The Uk

Seroprevalence ofCoxiella burnetii, the causal agent of Q fever in humans, was examined in wild brown rat populations from 4 Oxfordshire farmsteads (n= 127), 9 Somerset homesteads (n= 98), and 1 captive enclosure (n= 88) in the UK. Seroprevalence ranged between 7 and 53%, the lowest being within the captive population and the highest on dairy farmsteads with sheep present. This is the first reported investigation ofC. burnetiiin wild rats outside India. We suggest that the high Seroprevalence among rats (i) provides evidence that wild rats constitute an important reservoir forC. burnetiiin the UK, and (ii) explains why cats, as frequent predators of rats, are so important in the epidemiology of this disease.

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Seroprevalence of Q Fever in Lorestan Province Veterinary Staff Using IFA Method

International Journal of Infection ◽

10.5812/iji.110731 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Zahra Soleimani ◽

Amin Jaydari

Keyword(s):

Coxiella Burnetii ◽

Work Experience ◽

Q Fever ◽

Cross Sectional Study ◽

Sectional Study ◽

High Seroprevalence ◽

Cross Sectional ◽

Treatment And Prevention ◽

Significant Relationships ◽

History Of

Background: Q fever is caused by the bacterium Coxiella burnetii. To date, no studies have been conducted on the rate of Q fever infection among the veterinary staff in Lorestan province. Objectives: The present study aimed to investigate the seroprevalence of infection in the veterinary staff. Methods: This cross-sectional study was conducted from April to July 2019 on 92 samples collected randomly from Lorestan province on veterinary staff who had a history of contact with livestock and were tested via indirect immunofluorescence kit. Results: In this study, 77 (83/69%) samples were positive, and 15 (16/3%) samples were negative. There were no statistically significant relationships between residence, contact with livestock, and work experience. Conclusions: According to the results of this study, the high seroprevalence of a Q fever was observed among Veterinary staff, which further reveals the necessitates of diagnosis, treatment, and prevention of the disease in order to prevent unwanted complications in people in contact with livestock.

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Seroreactivity to Coxiella burnetii in an Agricultural Population and Prevalence of Coxiella burnetii Infection in Ticks of A Non-Endemic Region for Q Fever in South Korea

Pathogens ◽

10.3390/pathogens10101337 ◽

2021 ◽

Vol 10 (10) ◽

pp. 1337

Author(s):

Jeong-Rae Yoo ◽

Mi-Sun Kim ◽

Sang-Taek Heo ◽

Hyun-Joo Oh ◽

Jung-Hwan Oh ◽

...

Keyword(s):

South Korea ◽

Coxiella Burnetii ◽

Ribosomal Rna ◽

Rural Areas ◽

Q Fever ◽

Jeju Island ◽

Haemaphysalis Longicornis ◽

Igg Antibodies ◽

High Seroprevalence ◽

Endemic Region

Coxiella burnetii infects humans and wild and domesticated animals. Although reported cases on Jeju Island, off the coast of South Korea, are rare, the region is considered to have a high potential for Q fever. We investigated the seroprevalence of antibodies to C. burnetii in 230 farmers living in ten rural areas on Jeju Island between January 2015 and December 2019. Blood samples were collected and examined for C. burnetii Phase I/II IgM and IgG antibodies. Trained researchers collected ticks from rural areas. Clone XCP-1 16S ribosomal RNA gene sequencing was performed to identify Coxiella species from the collected ticks. The overall seroprevalence of antibodies to C. burnetii in farmers was 35.7%. The seroprevalence was significantly higher in fruit farmers. Of the collected ticks, 5.4% (19/351) of the Haemaphysalis longicornis ticks harbored C. burnetti. A high seroprevalence of antibodies to C. burnetii was observed in this region of Jeju Island, confirming that C. burnetti is endemic. Physicians should thus consider Q fever in the differential diagnosis of patients that present with acute fever after participating in outdoor activities.

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A comparison of three cryotechniques (freeze-drying, cryosubstitution, cryosectioning) of specimen preparation for immunolabelling of bacterial antigens of Coxiella burnetii

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100168438 ◽

1994 ◽

Vol 52 ◽

pp. 140-141

Author(s):

T. F. McCaul ◽

R. J. Gould

Keyword(s):

Coxiella Burnetii ◽

Freeze Drying ◽

Stress Proteins ◽

Cultured Cells ◽

High Specificity ◽

Specimen Preparation ◽

Bacterial Antigens ◽

Structural Preservation ◽

Nuclear Ribonucleoprotein ◽

Cellular Components

Immuno-electron microscopy has allowed the selective localisation of molecules with high resolution and high specificity. Cryopreparatory methods have provided better retention of antigenicity suitable for precise immunolabelling together with optimal structural preservation of cellular components. Cryosubstitution and cryoultramicrotomy have widely been exploited for immunolabelling. Molecular Distillation Dryer (MDD), a form of freeze-drying technique, has recently been used for immunolabelling of Plasmodium falciparum stress proteins and nuclear ribonucleoprotein particles in cultured cells. In the present study, we report the comparison of all three cryotechniques in the immunolabelling of bacterial antigens of Coxiella burnetii.The highly infectious C. burnetii was prefixed in 3% glutaraldehyde (66 mM cacodylate buffer, pH 6.8 ). The cells were then pre-embedded in 2% low-temperature agarose on Durapore hydrophilic membrane prior to cryofixation using a LifeCell CF100 metal-mirror system. A 1% glutaraldehyde in 100% methanol was used as a medium for cryosubstitution in a Reichert CS Auto Cryosubstitution apparatus.

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