Loss of heterozygosity of APC and DCC tumor suppressor genes in human sporadic colon cancer

1999 ◽  
Vol 77 (3) ◽  
pp. 316-321 ◽  
Author(s):  
Sanda Šturlan ◽  
S. Kapitanović ◽  
Duje Kovačević ◽  
Josip Lukač ◽  
Šime Spaventi ◽  
...  
Keyword(s):  
Colon Cancer ◽  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Suppressor Genes ◽  
Sporadic Colon Cancer

Analysis of loss of heterozygosity on chromosomes 10q, 11, and 16 in medulloblastomas

2001 ◽  
Vol 94 (5) ◽  
pp. 799-805 ◽  
Author(s):  
Xiao-lu Yin ◽  
Jesse C. Pang ◽  
Yan-hui Liu ◽  
Edith Y. Chong ◽  
Yue Cheng ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Molecular Genetic ◽  
Genetic Material ◽  
Suppressor Genes ◽  
Blood Samples ◽  
Content Type ◽  
Targeted Deletion ◽  
Fine Print

Object. The loss of genetic material from specific chromosome loci is a common feature in the oncogenesis of tumors and is often indicative of the presence of important tumor suppressor genes at these loci. Recent molecular genetic analyses have demonstrated frequent loss of chromosomes 10q, 11, and 16 in medulloblastomas. The aim of this study was to localize the targeted deletion regions on the three aforementioned chromosomes in medulloblastomas. Methods. Loss of heterozygosity (LOH) was examined on chromosomes 10q, 11, and 16 in a series of 22 primary and two recurrent medulloblastomas by using polymerase chain reaction—based microsatellite analysis. The DNA extracted from the tumors and corresponding normal blood samples were amplified independently in the presence of radioactively labeled microsatellite primers, resolved by denaturing gel electrophoresis and processed for autoradiography. The DNA obtained from control blood samples that displayed allelic heterozygosity at a given microsatellite locus were considered informative. Loss of heterozygosity was inferred when the allelic signal intensity of the tumor sample was reduced by at least 40%, relative to that of the constitutional control. The LOH analysis demonstrated that deletions of chromosomes 10q, 11p, and 16q are recurrent genetic events in the development of medulloblastomas. Three subchromosomal regions of loss have been identified and are localized to the deleted in malignant brain tumors 1 [DMBT1] gene site on chromosomes 10q25, 11p13–11p15.1, and 16q24.1–24.3. Conclusions. These results indicate that DMBT1 is closely associated with the oncogenesis of medulloblastomas and highlight regions of loss on chromosomes 11p and 16q for further fine mapping and cloning of candidate tumor suppressor genes that are important for the genesis of medulloblastoma.

Analysis of Loss of Heterozygosity for Tumor-Suppressor Genes Can Accurately Classify and Predict the Clinical Behavior of Mucinous Tumors Arising From the Appendix

2006 ◽  
Vol 13 (12) ◽  
pp. 1610-1616 ◽  
Author(s):  
Vivek Maheshwari ◽  
Allan Tsung ◽  
Yan Lin ◽  
Herb J. Zeh ◽  
Sydney D. Finkelstein ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Suppressor Genes ◽  
Clinical Behavior

Frequent Occurrence of Loss of Heterozygosity among Tumor Suppressor Genes in Uterine Leiomyosarcoma

1999 ◽  
Vol 75 (3) ◽  
pp. 453-459 ◽  
Author(s):  
Ya-Li Zhai ◽  
Toshio Nikaido ◽  
Ayaka Orii ◽  
Akiko Horiuchi ◽  
Toshihiko Toki ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Frequent Occurrence ◽  
Uterine Leiomyosarcoma ◽  
Suppressor Genes

scholarly journals Loss of Heterozygosity at the Ink4a/ArfLocus Facilitates Abelson Virus Transformation of Pre-B Cells

2000 ◽  
Vol 74 (20) ◽  
pp. 9479-9487 ◽  
Author(s):  
Justin Mostecki ◽  
Anne Halgren ◽  
Arash Radfar ◽  
Zohar Sachs ◽  
James Ravitz ◽  
...  
Keyword(s):  
B Cells ◽  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Cell Lines ◽  
Tumor Suppressor Genes ◽  
Single Copy ◽  
Transformation Process ◽  
Suppressor Genes ◽  
Abelson Virus

ABSTRACT In many tumor systems, analysis of cells for loss of heterozygosity (LOH) has helped to clarify the role of tumor suppressor genes in oncogenesis. Two important tumor suppressor genes, p53 and the Ink4a/Arf locus, play central roles in the multistep process of Abelson murine leukemia virus (Ab-MLV) transformation. p53 and the p53 regulatory protein, p19Arf, are required for the apoptotic crisis that characterizes the progression of primary transformed pre-B cells to fully malignant cell lines. To search for other tumor suppressor genes which may be involved in the Ab-MLV transformation process, we used endogenous proviral markers and simple-sequence length polymorphism analysis to screen Abelson virus-transformed pre-B cells for evidence of LOH. Our survey reinforces the role of the p53-p19 regulatory pathway in transformation; 6 of 58 cell lines tested had lost sequences on mouse chromosome 4, including theInk4a/Arf locus. Consistent with this pattern, a high frequency of primary pre-B-cell transformants derived fromInk4a/Arf +/− mice became established cell lines. In addition, half of them retained the single copy of the locus when the transformation process was complete. These data demonstrate that a single copy of the Ink4a/Arf locus is not sufficient to fully mediate the effects of these genes on transformation.

Loss of heterozygosity of selected tumor suppressor genes in parathyroid carcinoma

Surgery ◽  
2008 ◽  
Vol 144 (6) ◽  
pp. 949-955 ◽  
Author(s):  
Linwah Yip ◽  
Raja R. Seethala ◽  
Marina N. Nikiforova ◽  
Yuri E. Nikiforov ◽  
Jennifer B. Ogilvie ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Parathyroid Carcinoma ◽  
Suppressor Genes

Use of Crypt Isolation to Determine Loss of Heterozygosity of Multiple Tumor Suppressor Genes in Colorectal Carcinoma

2000 ◽  
Vol 196 (3) ◽  
pp. 145-150 ◽  
Author(s):  
Tamotsu Sugai ◽  
Wataru Habano ◽  
Shin-ichi Nakamura ◽  
Toru Yoshida ◽  
Noriyuki Uesugi ◽  
...  
Keyword(s):  
Tumor Suppressor ◽  
Colorectal Carcinoma ◽  
Loss Of Heterozygosity ◽  
Tumor Suppressor Genes ◽  
Suppressor Genes ◽  
Multiple Tumor ◽  
Crypt Isolation
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