scholarly journals Genetic variation and inheritance of phytosterol and oil content in a doubled haploid population derived from the winter oilseed rape Sansibar × Oase cross

2015 ◽  
Vol 129 (1) ◽  
pp. 181-199 ◽  
Author(s):  
Lishia Teh ◽  
Christian Möllers
Keyword(s):  
Genetic Variation ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Oil Content ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Haploid Population

Mapping QTL controlling agronomic traits in a doubled haploid population of winter oilseed rape (Brassica napus L.)

2018 ◽  
Vol 97 (5) ◽  
pp. 1389-1406 ◽  
Author(s):  
Farshad Fattahi ◽  
Barat Ali Fakheri ◽  
Mahmood Solouki ◽  
Christian Möllers ◽  
Abbas Rezaizad
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Agronomic Traits ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Brassica Napus L ◽  
Mapping Qtl ◽  
Haploid Population

Mapping of QTL for seed dormancy in a winter oilseed rape doubled haploid population

2013 ◽  
Vol 126 (9) ◽  
pp. 2405-2415 ◽  
Author(s):  
Jörg Schatzki ◽  
Burkhard Schoo ◽  
Wolfgang Ecke ◽  
Cornelia Herrfurth ◽  
Ivo Feussner ◽  
...  
Keyword(s):  
Seed Dormancy ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Doubled Haploid Population ◽  
Winter Oilseed Rape ◽  
Haploid Population

scholarly journals A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape

2020 ◽  
Vol 71 (18) ◽  
pp. 5402-5413
Author(s):  
Jia Liu ◽  
Rijin Zhou ◽  
Wenxiang Wang ◽  
Hui Wang ◽  
Yu Qiu ◽  
...  
Keyword(s):  
Oilseed Rape ◽  
Doubled Haploid ◽  
Quantitative Variation ◽  
Upstream Region ◽  
Doubled Haploid Population ◽  
Parental Line ◽  
Pod Shattering ◽  
Retrotransposon Insertion ◽  
Pod Shatter ◽  
Haploid Population

Abstract Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B. napus SHATTERPROOF1 homologs, BnSHP1.A9, in a doubled haploid population generated from parents designated R1 and R2 as well as in a diverse panel of oilseed rape. The R1 maternal parental line of the doubled haploid population carried the allele for shattering at qSRI.A9.1, while the R2 parental line carried the allele for shattering resistance. Quantitative RT-PCR showed that BnSHP1.A9 was expressed specifically in flower buds, flowers, and developing siliques in R1, while it was not expressed in any tissue of R2. Transgenic plants constitutively expressing either of the BnSHP1.A9 alleles from the R1 and R2 parental lines showed that both alleles are responsible for pod shattering, via a mechanism that promotes lignification of the enb layer. These findings indicated that the allelic differences in the BnSHP1.A9 gene per se are not the causal factor for quantitative variation in shattering resistance at qSRI.A9.1. Instead, a highly methylated copia-like long terminal repeat retrotransposon insertion (4803 bp) in the promotor region of the R2 allele of BnSHP1.A9 repressed the expression of BnSHP1.A9, and thus contributed to pod shatter resistance. Finally, we showed a copia-like retrotransposon-based marker, BnSHP1.A9R2, can be used for marker-assisted breeding targeting the pod shatter resistance trait in oilseed rape.

scholarly journals Clubroot Symptoms and Resting Spore Production in a Doubled Haploid Population of Oilseed Rape (Brassica napus) Are Controlled by Four Main QTLs

2020 ◽  
Vol 11 ◽  
Author(s):  
Andrea Botero-Ramírez ◽  
Anne Laperche ◽  
Solenn Guichard ◽  
Mélanie Jubault ◽  
Antoine Gravot ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Oilseed Rape ◽  
Doubled Haploid ◽  
Plasmodiophora Brassicae ◽  
Spore Production ◽  
Doubled Haploid Population ◽  
Resting Spore ◽  
Monogenic Resistance ◽  
Pathogen Fitness ◽  
Haploid Population

Clubroot, caused by Plasmodiophora brassicae Woronin, is one of the most important diseases of oilseed rape (Brassica napus L.). The rapid erosion of monogenic resistance in clubroot-resistant (CR) varieties underscores the need to diversify resistance sources controlling disease severity and traits related to pathogen fitness, such as resting spore production. The genetic control of disease index (DI) and resting spores per plant (RSP) was evaluated in a doubled haploid (DH) population consisting of 114 winter oilseed rape lines, obtained from the cross ‘Aviso’ × ‘Montego,’ inoculated with P. brassicae isolate “eH.” Linkage analysis allowed the identification of three quantitative trait loci (QTLs) controlling DI (PbBn_di_A02, PbBn_di_A04, and PbBn_di_C03). A significant decrease in DI was observed when combining effects of the three resistance alleles at these QTLs. Only one QTL, PbBn_rsp_C03, was found to control RSP, reducing resting spore production by 40%. PbBn_rsp_C03 partially overlapped with PbBn_di_C03 in a nucleotide-binding leucine-rich repeat (NLR) gene-containing region. Consideration of both DI and RSP in breeding for clubroot resistance is recommended for the long-term management of this disease.

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