Identification and grouping of pod shattering resistance and agronomic characters performance from several soybean advanced lines

The use of pod-shatter resistant variety is considered effective in reducing yield loss in soybeans. This study aimed to identify and grouping of pod shattering resistance and agronomic characters performance from several advanced soybean lines. The field experiment was conducted in Blitar (Indonesia) from January to April 2021, using 20 soybean advanced lines and two check cultivars. The laboratory study to identify the pod shattering resistance based on the oven-dry method was conducted in the Breeding Laboratory of ILETRI, Malang. The pod shattering resistance of 20 advanced lines was classified into five very resistant genotypes, three resistant genotypes, two moderate genotypes, nine susceptible genotypes, and a very susceptible genotype. The pod shattering resistance has a significant negative correlation with the pod width (r = -0.472*). A wider pod resulted in a low percentage of shattering. The agronomic performance varied among all genotypes. Selection for pod shattering resistance and agronomic performance obtained two lines (G511H/Anjas//Anjas///Anjas-3-1 and Dega/Degra-2-105) as high yielding and very resistant to pod shattering. Those lines were suggested to be tested across diverse environments to determine their yield potential and stability.

Gene editing in Brassica napus for basic research and trait development

The genome of Brassica napus L. is the result of several polyploidization events that occurred during the history of B. napus. Due to its relatively short domestication history, diversity is relatively limited. An increasing number of loci in this crop’s genome have been gene-edited using various technologies and reagent delivery methods for basic research as well as for trait development. New alleles have been developed as edits in single, 2, 4, or more homologous loci in this important oilseed crop. This comprehensive review will summarize new alleles that have been developed as they relate to weed control, flowering, self-incompatibility, plant hormone biology, disease resistance, grain composition, and pod shatter reduction. These new alleles have significantly augmented our understanding of both plant growth and development for basic research as well as for their potential commercial impacts.

Screen Oilseed Rape (Brassica napus) Suitable for Low-Loss Mechanized Harvesting

The main reason for the massive loss of rapeseed in mechanized harvesting is the mismatch between the harvester requirements and the pod shatter resistance and plant branching characteristics. Low pod resistance, entanglement caused by excessive branches, and inconsistent pod maturity are primary mismatch problems. However, studies on rape characteristics by integrating agricultural machinery and agronomy are limited. A total of 15 varieties were planted for research from 2016 to 2018. In this paper, the Two-Degree-of-Freedom (2-DOF) collision method was adopted to evaluate the pod shatter resistance taken from the field, and the plant agronomic characteristics and their correlation were investigated. In 2020, a screened variety of C6009 with higher shatter resistance and suitable plant features for mechanized harvesting was planted in large areas and harvested by machines for verification. The test results demonstrated that the compact plant varieties with high branches might be more favorable for yield and shatter resistance. The field harvest loss of the screened variety was significantly less than that of the control group. It provides a reliable reference for agronomic experts in terms of rape variety improvement and agricultural machinery experts regarding the optimization of rape harvesters.

Ascophyllum nodosum Extract (SealicitTM) Boosts Soybean Yield Through Reduction of Pod Shattering-Related Seed Loss and Enhanced Seed Production

Soybean is one of the most valuable commercial crops because of its high protein, carbohydrate, and oil content. The land area cultivated with soybean in subtropical regions, such as Brazil, is continuously expanding, in some instances at the expense of carbon storing natural habitats. Strategies to decrease yield/seed losses and increase production efficiency are urgently required to meet global demand for soybean in a sustainable manner. Here, we evaluated the effectiveness of an Ascophyllum nodosum extract (ANE), SealicitTM, in increasing yields of different soybean varieties, in two geographical regions (Canada and Brazil). In addition, we investigated the potential of SealicitTM to reduce pod shattering at the trials in Brazil. Three different concentrations of SealicitTM were applied to pod shatter-susceptible (SS) UFUS 6901 and shatter-resistant (SR) UFUS 7415 varieties to assess their impact on pod firmness. SS variety demonstrated a significant decrease in pod shattering, which coincided with deregulation of GmPDH1.1 and GmSHAT1–5 expression, genes that determine pod dehiscence, and higher seed weight per pod. SealicitTM application to the SR variety did not significantly alter its inherent pod shatter resistance, but provided higher increases in seed yield at harvest. This yield increase maybe associated with to other yield components stimulated by the biostimulant. This work demonstrates that SealicitTM, which has previously been shown to improve pod firmness in Arabidopsis and selected commercial oilseed rape varieties through IND gene down-regulation, also has the potential to improve pod resistance and seed productivity in soybean, a member of the legume family sharing a similar strategy for seed dispersal.

Dependence of soy growth processes on the application of fungicides and inoculants in the Right bank Forest steppe of Ukraine

The article presents the results of the research dealing with the dependence of the mass, growth processes and the attachment height of the low bean of soybean under the application of different types of fungicides and biopreparation Rhizoactive in the Right Bank Forest-Steppe of Ukraine. Application of different types of fungicides had a positive influence on the plant mass of soybeans, growth processes and attachment height of a low bean. Indexes were defined in different years and at different development stages of a plant: budding, flowering and beans maturing. Throughout all development stages, the best indexes of soybeans height, their mass and attachment height of a low bean were under the complex application of preparations, in particular, the highest indexes were in the variants with the application of fungicides Impact K, 0,9l/ha; Koronet 300 SC, KC 0.8 l/ha at the background of seeds treatment with the inoculator Rhizoactove. Important selection features that are associated with the main morphological and biological characteristics of soybeans are the height, mass and lower bean attachment height of soybean. Productivity in general depends on the height and weight of plants, as the stem is an organ of conversion and transport of organic and mineral substances, which plays an important role in crop formation. Technology of soybean cultivation on irrigation provides suitability of varieties for mechanized harvesting, including resistance to pod shatter and lodging, optimal placement of the first beans on the plant and genotypes reaction to irrigation. For successful introduction into production, new soybean varieties must be not only high yielding but also suitable for mechanized harvesting, which is primarily due to the lower bean attachment height of the plant. Low first bean attachment leads to a decrease in the yield of the variety, since a significant number of beans are lost during combine harvesting. Yield losses because of the low attachment of the lower bean may reach 15-20%. This feature is associated with the total height of the plant. The height of soybean plants and the lower bean attachment largely depend on growing conditions. The research conducted by A.Ya Ala. and A.A. Hamolin have established that linear parameters of plants at irrigation considerably increase. In the south of Ukraine irrig

A copia-like retrotransposon insertion in the upstream region of the SHATTERPROOF1 gene, BnSHP1.A9, is associated with quantitative variation in pod shattering resistance in oilseed rape

Seed loss resulting from pod shattering is a major constraint in production of oilseed rape (Brassica napus L.). However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here, we show that the pod shatter resistance at quantitative trait locus qSRI.A9.1 is controlled by one of the B. napus SHATTERPROOF1 homologs, BnSHP1.A9, in a doubled haploid population generated from parents designated R1 and R2 as well as in a diverse panel of oilseed rape. The R1 maternal parental line of the doubled haploid population carried the allele for shattering at qSRI.A9.1, while the R2 parental line carried the allele for shattering resistance. Quantitative RT-PCR showed that BnSHP1.A9 was expressed specifically in flower buds, flowers, and developing siliques in R1, while it was not expressed in any tissue of R2. Transgenic plants constitutively expressing either of the BnSHP1.A9 alleles from the R1 and R2 parental lines showed that both alleles are responsible for pod shattering, via a mechanism that promotes lignification of the enb layer. These findings indicated that the allelic differences in the BnSHP1.A9 gene per se are not the causal factor for quantitative variation in shattering resistance at qSRI.A9.1. Instead, a highly methylated copia-like long terminal repeat retrotransposon insertion (4803 bp) in the promotor region of the R2 allele of BnSHP1.A9 repressed the expression of BnSHP1.A9, and thus contributed to pod shatter resistance. Finally, we showed a copia-like retrotransposon-based marker, BnSHP1.A9R2, can be used for marker-assisted breeding targeting the pod shatter resistance trait in oilseed rape.

An Epigenetic LTR-retrotransposon insertion in the upstream region of BnSHP1.A9 controls quantitative pod shattering resistance in Brassica napus

Seed loss resulting from pod shattering is a major problem in oilseed rape (Brassica napus L.) production worldwide. However, the molecular mechanisms underlying pod shatter resistance are not well understood. Here we show that the pod shatter resistance at quantitative trait locus, qSRI.A9.1 is controlled by a SHATTERPROOF1 (SHP1) paralog in B. napus (BnSHP1.A9). Expression analysis by quantitative RT-PCR showed that BnSHP1.A9 was specifically expressed in flower buds, flowers and developing siliques in the oilseed rape line (R1) carrying the qSRI.A9.1 allele with negative effect, but not expressed in any tissue of the line (R2) carrying the positive effect qSRI.A9.1 allele. Transgenic plants constitutively expressing BnSHP1.A9 alleles from pod resistant and pod shattering parental lines showed that both alleles are responsible for pod shattering via promoting lignification of enb layer, which indicated allelic difference of BnSHP1.A9 gene per se is not the causal factor of the QTL. The upstream sequence of BnSHP1.A9 in the promotor region harboring highly methylated long terminal repeat retrotransposon insertion (LTR, 4803bp) in R2 repressed the expression of BnSHP.A9, and thus contributed to the positive effect on pod shatter resistance. Genetic and association analysis revealed that the copia LTR retrotransposon based marker BnSHP1.A9-R2 can be used for breeding for pod shatter resistant varieties and reducing the loss of seed yield in oilseed rape.