Fine-mapping and gene candidate analysis for AhRt1, a major dominant locus responsible for testa color in cultivated peanut

Abstract A better understanding of peanut (Arachis hypogaea L.) testa color genetics would be helpful to breeders in developing new cultivars to meet U.S. market acceptability. Wine is one of the least understood of all basic testa colors in peanut. The objective of this genetic study was to gain further knowledge on the inheritance of wine testa color and possible allelic interactions. Crosses were made using two true-breeding wine testa color genotypes (Wine-Frr and PI 264549) as females with the tan testa and recessive red testa male parents Krinkle-Leaf and Makulu Red, respectively. F1, F2, and F3 data suggest no difference between the two wine testa color genotypes. Inheritance of wine testa color was found to be recessive with a one gene difference between wine and the tan testa color of Krinkle-Leaf, and with two gene differences between wine and the recessive red testa color of Makulu Red. Inheritance of wine seems to closely parallel that for recessive red testa color in the cultivated peanut.

Download Full-text

Inheritance of White-Spot Testa Color Trait in Peanut1

Peanut Science ◽

10.3146/i0095-3679-25-1-11 ◽

1998 ◽

Vol 25 (1) ◽

pp. 44-45 ◽

Cited By ~ 1

Author(s):

W. D. Branch

Keyword(s):

Arachis Hypogaea ◽

White Spot ◽

Arachis Hypogaea L ◽

True Breeding ◽

Cultivated Peanut ◽

Recessive Genes ◽

Testa Color

Abstract The white-spot testa color trait has occasionally been found among segregating cross populations in the cultivated peanut (Arachis hypogaea L.). Crosses involving one such true-breeding white-spot selection were made both between and within subspecies of the cultigen to determine the inheritance of this unusual trait. The F1, F2, and F3 data indicated that two duplicate recessive genes, designated wsp1 and wsp2, control the white-spot trait in peanut.

Download Full-text

Genic Relationship Between R1, R2, and R3 for Red Peanut Testa Color1

Peanut Science ◽

10.3146/i0095-3679-15-1-4 ◽

1988 ◽

Vol 15 (1) ◽

pp. 13-14 ◽

Cited By ~ 3

Author(s):

W. D. Branch ◽

C. C. Holbrook

Keyword(s):

Arachis Hypogaea ◽

The Other ◽

Genetic Characteristic ◽

Reciprocal Crosses ◽

Arachis Hypogaea L ◽

Cultivated Peanut ◽

R1 Gene ◽

Testa Color

Abstract Testa color in the cultivated peanut (Arachis hypogaea L.) is an important genetic characteristic. Presently, three genes (R1, R2, and R3) are known to be involved in the expression of red testa color. Reciprocal crosses between the dominant (R1 R1) Tennessee Red and recessive (r2 r2) Makulu Red cultivars and test crosses between Makulu Red and two recessive red genotypes (r3 r3) were made to determine the interaction among these three loci. The F1, F2, and F3 results suggest that the red testa color of Tennessee Red differs from that of Makulu Red by two loci and that Makulu Red does not differ from the other recessive red genotypes. Also, the R1 gene appears to be inherited independently from at least one of the recessive alleles controlling red peanut testa color.

Download Full-text

IntroMap: A Pipeline and Set of Diagnostic Diploid Arachis SNPs as a Tool for Mapping Alien Introgressions in Arachis hypogaea

Peanut Science ◽

10.3146/ps17-5.1 ◽

2017 ◽

Vol 44 (2) ◽

pp. 66-73 ◽

Cited By ~ 6

Author(s):

J. Clevenger ◽

D.J. Bertioli ◽

S.C.M. Leal-Bertioli ◽

Y. Chu ◽

H.T. Stalker ◽

...

Keyword(s):

Arachis Hypogaea ◽

Fine Mapping ◽

Related Species ◽

Mapping Algorithms ◽

Alien Introgression ◽

Genetic Base ◽

Public Resources ◽

Cultivated Peanut ◽

Low Coverage ◽

Diagnostic Snps

ABSTRACT For crops with a narrow cultivated genetic base, incorporating beneficial alleles from related species through alien introgression widens the genetic base and provides key resistances to disease and abiotic stresses. Fine mapping of these introgressions can increase the efficiency of marker-assisted selection for breeding programs. To facilitate high resolution fine mapping of alien introgressions, we developed an automated pipeline, IntroMap. This pipeline was developed with accessibility and utility in mind, and does not present novel mapping algorithms. Using five diploid wild Arachis species, we identified diagnostic SNP sets for introgression mapping in Arachis hypogaea, cultivated peanut. IntroMap has applicability in all crops where alien introgression is used to bring in beneficial alleles from related species, so the pipeline includes an option to generate new diagnostic SNPs from any species/accession of interest for use in the pipeline. These user generated resources will be included for distribution with IntroMap to increase the SNP resources for all users. We demonstrate the efficacy of IntroMap by fine mapping three alien introgressions in an elite peanut breeding line with superior disease resistance. IntroMap works well even at low coverage, recovering at 2x coverage almost 50% of the diagnostic SNPs found at 10x coverage. The true benefit of IntroMap is the availability and generation of shared public resources, specifically for Arachis spp. IntroMap is freely distributed at https://sourceforge.net/projects/intromap/.

Download Full-text