Genetic linkage map of peach [Prunus persica (L.) Batsch] using morphological and molecular markers

1998 ◽  
Vol 97 (5-6) ◽  
pp. 888-895 ◽  
Author(s):  
E. Dirlewanger ◽  
V. Pronier ◽  
C. Parvery ◽  
C. Rothan ◽  
A. Guye ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Prunus Persica

Construction of a genetic linkage map and identification of AFLP markers for resistance to root-knot nematodes in peach rootstocks

Genome ◽  
1998 ◽  
Vol 41 (2) ◽  
pp. 199-207 ◽  
Author(s):  
Zhen-Xiang Lu ◽  
B Sosinski ◽  
G L Reighard ◽  
W V Baird ◽  
A G Abbott
Keyword(s):  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Prunus Persica ◽  
Aflp Marker ◽  
Aflp Markers ◽  
Single Linkage ◽  
Root Knot Nematodes ◽  
Group I ◽  
Peach Rootstocks

A genetic linkage map for peach (Prunus persica (L.) Batsch) rootstocks has been constructed using amplified fragment length polymorphism (AFLP) markers in 55 F2 individuals derived from the cross Lovell x Nemared. From 21 different primer combinations, a total of 169 AFLP markers were scored, of which, 153 were assigned to 15 linkage groups covering 1297 centimorgans (cM) of the peach nuclear genome. The average interval between these markers was 9.1 cM. Two genes (Mi and Mij) involved in resistance to root-knot nematodes (Meloidogyne incognita (Kofoid and White) Chitwood and Meloidogyne javanica (Treub) Chitwood) were mapped to a single linkage group (Group I). These two loci were separated by a 16.5-cM interval. One codominant AFLP marker (EAA/MCAT10) was tightly linked to the Mij locus (3.4 cM), and a dominant AFLP marker (EAT/MCAT2) was found to be closely associated with the Mi locus (6.0 cM). These markers are being studied for utilization in peach rootstock breeding with marker-assisted selection.Key words: peach rootstocks, root-knot nematodes, resistance, AFLP, mapping.

Application of the random amplified polymorphic DNA technique for the detection of polymorphism among wild and cultivated tetraploid wheats

Genome ◽  
1993 ◽  
Vol 36 (3) ◽  
pp. 602-609 ◽  
Author(s):  
Chandrashekhar P. Joshi ◽  
Henry T. Nguyen
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Triticum Turgidum ◽  
Rapd Analysis ◽  
Genetic Relationships ◽  
Random Amplified Polymorphic Dna ◽  
Emmer Wheat ◽  
Tetraploid Wheats

Development of a high-density genetic linkage map of cultivated wheats using conventional molecular markers has lagged behind the other major food crops such as rice and tomato because of the large genome size and limited levels of genetic polymorphisms. Recently, random amplified polymorphic DNAs (RAPDs) have been suggested to provide an alternative to visualize more polymorphism. For the construction of a genetic linkage map in tetraploid wheats, one can use a strategy of intersubspecific crosses between the most dissimilar wild and cultivated tetraploid wheats that are easy to hybridize and result in fertile progeny. An assessment of the level of RAPDs among different accessions and varieties of wild and cultivated tetraploid wheats is required to fulfill this objective. We present here the data obtained using RAPD analysis of 40 primers in 20 accessions of wild tetraploid emmer wheats (Triticum turgidum L. ssp. dicoccoides) and 10 genotypes of cultivated tetraploid durum wheats (Triticum turgidum L. ssp. durum) selected from geographically diverse locations. We have observed a higher level of polymorphism among different accessions of wild emmer wheat from Israel, Turkey, and Jordan than the group of cultivated American, Turkish, and Syrian durum wheats. These data have been used to generate a dendrogram suggesting the genetic relationships among these genotypes, and the most dissimilar genotypes are identified for future mapping and gene tagging work.Key words: durum wheat, emmer wheat, genetic similarity, molecular markers, RAPD analysis.

Construction of a genetic linkage map of black gram, Vigna mungo (L.) Hepper, based on molecular markers and comparative studies

Genome ◽  
2008 ◽  
Vol 51 (8) ◽  
pp. 628-637 ◽  
Author(s):  
S. K. Gupta ◽  
J. Souframanien ◽  
T. Gopalakrishna
Keyword(s):  
Molecular Markers ◽  
Linkage Group ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Average Distance ◽  
Vigna Mungo ◽  
Black Gram ◽  
Recombinant Inbred Population ◽  
Average Marker Density

A genetic linkage map of black gram, Vigna mungo (L.) Hepper, was constructed with 428 molecular markers using an F9 recombinant inbred population of 104 individuals. The population was derived from an inter-subspecific cross between a black gram cultivar, TU94-2, and a wild genotype, V. mungo var. silvestris. The linkage analysis at a LOD score of 5.0 distributed all 428 markers (254 AFLP, 47 SSR, 86 RAPD, and 41 ISSR) into 11 linkage groups. The map spanned a total distance of 865.1 cM with an average marker density of 2 cM. The largest linkage group spanned 115 cM and the smallest linkage group was of 44.9 cM. The number of markers per linkage group ranged from 11 to 86 and the average distance between markers varied from 1.1 to 5.6 cM. Comparison of the map with other published azuki bean and black gram maps showed high colinearity of markers, with some inversions. The current map is the most saturated map for black gram to date and will provide a useful tool for identification of QTLs and for marker-assisted selection of agronomically important characters in black gram.

Construction of a genetic linkage map and identification of molecular markers in peach rootstocks for response to peach tree short life syndrome

2007 ◽  
Vol 3 (4) ◽  
pp. 341-350 ◽  
Author(s):  
A. V. Blenda ◽  
I. Verde ◽  
L. L. Georgi ◽  
G. L. Reighard ◽  
S. D. Forrest ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Peach Tree ◽  
Short Life ◽  
Peach Rootstocks

A genetic linkage map of crested wheatgrass based on AFLP and RAPD markers

Genome ◽  
2012 ◽  
Vol 55 (4) ◽  
pp. 327-335 ◽  
Author(s):  
Xiaoxia Yu ◽  
Xiaolei Li ◽  
Yanhong Ma ◽  
Zhuo Yu ◽  
Zaozhe Li
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Mapping Population ◽  
Gene Localization ◽  
Linkage Groups ◽  
Crested Wheatgrass ◽  
Map Distance

Using a population of 105 interspecific F2 hybrids derived from a cross between Agropyron mongolicum Keng and Agropyron cristatum (L.) Gaertn. ‘Fairway’ as a mapping population, a genetic linkage map of crested wheatgrass was constructed based on AFLP and RAPD molecular markers. A total of 175 markers, including 152 AFLP and 23 RAPD markers, were ordered in seven linkage groups. The map distance was 416 cM, with a mean distance of 2.47 cM between markers. The number of markers ranged from 13 to 46 in each linkage group and the length of groups ranged from 18 to 104 cM. The research found that 30 out of 175 molecular markers showed segregation distortion, accounting for 17% of all markers. This is the first genetic linkage map of crested wheatgrass. This map will facilitate gene localization, cloning, and molecular marker-assisted selection in the future.

Integration of Brassica A genome genetic linkage map between Brassica napus and B. rapa

Genome ◽  
2008 ◽  
Vol 51 (3) ◽  
pp. 169-176 ◽  
Author(s):  
Keita Suwabe ◽  
Colin Morgan ◽  
Ian Bancroft
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Linkage Groups ◽  
Indel Markers ◽  
A Genome ◽  
High Level ◽  
High Degree ◽  
Genetic Integration

An integrated linkage map between B. napus and B. rapa was constructed based on a total of 44 common markers comprising 41 SSR (33 BRMS, 6 Saskatoon, and 2 BBSRC) and 3 SNP/indel markers. Between 3 and 7 common markers were mapped onto each of the linkage groups A1 to A10. The position and order of most common markers revealed a high level of colinearity between species, although two small regions on A4, A5, and A10 revealed apparent local inversions between them. These results indicate that the A genome of Brassica has retained a high degree of colinearity between species, despite each species having evolved independently after the integration of the A and C genomes in the amphidiploid state. Our results provide a genetic integration of the Brassica A genome between B. napus and B. rapa. As the analysis employed sequence-based molecular markers, the information will accelerate the exploitation of the B. rapa genome sequence for the improvement of oilseed rape.

scholarly journals A Combined Amplified Fragment Length Polymorphism and Randomly Amplified Polymorphism DNA Genetic Linkage Map of Mycosphaerella graminicola, the Septoria Tritici Leaf Blotch Pathogen of Wheat

Genetics ◽  
2002 ◽  
Vol 161 (4) ◽  
pp. 1497-1505
Author(s):  
Gert H J Kema ◽  
Stephen B Goodwin ◽  
Sonia Hamza ◽  
Els C P Verstappen ◽  
Jessica R Cavaletto ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Linkage Map ◽  
Mating Type ◽  
Genetic Linkage ◽  
Rapd Markers ◽  
Genetic Linkage Map ◽  
Mycosphaerella Graminicola ◽  
Septoria Tritici ◽  
Biological Traits ◽  
Linkage Groups

Abstract An F1 mapping population of the septoria tritici blotch pathogen of wheat, Mycosphaerella graminicola, was generated by crossing the two Dutch field isolates IPO323 and IPO94269. AFLP and RAPD marker data sets were combined to produce a high-density genetic linkage map. The final map contained 223 AFLP and 57 RAPD markers, plus the biological traits mating type and avirulence, in 23 linkage groups spanning 1216 cM. Many AFLPs and some RAPD markers were clustered. When markers were reduced to 1 per cluster, 229 unique positions were mapped, with an average distance of 5.3 cM between markers. Because M. graminicola probably has 17 or 18 chromosomes, at least 5 of the 23 linkage groups probably will need to be combined with others once additional markers are added to the map. This was confirmed by pulsed-field gel analysis; probes derived from 2 of the smallest linkage groups hybridized to two of the largest chromosome-sized bands, revealing a discrepancy between physical and genetic distance. The utility of the map was demonstrated by identifying molecular markers tightly linked to two genes of biological interest, mating type and avirulence. Bulked segregant analysis was used to identify additional molecular markers closely linked to these traits. This is the first genetic linkage map for any species in the genus Mycosphaerella or the family Mycosphaerellaceae.

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