Inheritance and genetic mapping of cucumber mosaic virus resistance introgressed from Lycopersicon chilense into tomato

The Cucumber mosaic virus (CMV)-encoded 2b protein (Cmv2b) is a nuclear protein that suppresses transgene RNA silencing in Nicotiana benthamiana. Cmv2b is an important virulence determinant but nonessential for systemic spread in N. glutinosa, in contrast to its indispensable role for systemic infections in cucumber. Here, we report that Cmv2b became essential for systemic infections in older N. glutinosa plants or in young seedlings pre-treated with salicylic acid (SA). Expression of Cmv2b from the genome of either CMV or Tobacco mosaic virus significantly reduced the inhibitory effect of SA on virus accumulation in inoculated leaves and systemic leaves. A close correlation is demonstrated between Cmv2b expression and a reduced SA-dependent induction of the alternative oxidase gene, a component of the recently proposed SA-regulated antiviral defense. These results collectively reveal a novel activity of Cmv2b in the inhibition of SA-mediated virus resistance. We used a N. tabacum line expressing a bacterial nahG transgene that degrades SA to provide evidence for a Cmv2b-sensitive antiviral defense mechanism in tobacco in which SA acts as a positive modifier but not as an essential component. We propose that SA induces virus resistance by potentiating a RNA-silencing antiviral defense that is targeted by Cmv2b.

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Inheritance and QTL mapping of cucumber mosaic virus resistance in cucumber (Cucumis Sativus L.)

PLoS ONE ◽

10.1371/journal.pone.0200571 ◽

2018 ◽

Vol 13 (7) ◽

pp. e0200571 ◽

Cited By ~ 7

Author(s):

Lixue Shi ◽

Yuhong Yang ◽

Qing Xie ◽

Han Miao ◽

Kailiang Bo ◽

...

Keyword(s):

Qtl Mapping ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Cucumis Sativus ◽

Virus Resistance ◽

Cucumis Sativus L

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The role of synergistic action and molecular mechanism in the effect of genetically engineered strain Bacillus subtilis OKBHF in enhancing tomato growth and Cucumber mosaic virus resistance

BioControl ◽

10.1007/s10526-010-9306-x ◽

2010 ◽

Vol 56 (1) ◽

pp. 113-121 ◽

Cited By ~ 15

Author(s):

Shuai Wang ◽

Huijun Wu ◽

Jiang Zhan ◽

Yanfei Xia ◽

Shengfeng Gao ◽

...

Keyword(s):

Bacillus Subtilis ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Molecular Mechanism ◽

Virus Resistance ◽

Genetically Engineered ◽

Synergistic Action ◽

Tomato Growth ◽

Engineered Strain

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Genetic mapping of the compatibility between a lily isolate of Cucumber mosaic virus and a satellite RNA

Journal of General Virology ◽

10.1099/vir.0.81059-0 ◽

2005 ◽

Vol 86 (8) ◽

pp. 2359-2369 ◽

Cited By ~ 15

Author(s):

Naoya Yamaguchi ◽

Yuko Seshimo ◽

Eri Yoshimoto ◽

Hong Il Ahn ◽

Ki Hyun Ryu ◽

...

Keyword(s):

Amino Acid ◽

Genetic Mapping ◽

Cucumber Mosaic Virus ◽

Mosaic Virus ◽

Source Material ◽

Pronounced Effect ◽

Amino Acid Residues ◽

Satellite Rna ◽

Rna Sequence ◽

Ordinary Strain

Five isolates of Cucumber mosaic virus (CMV) from Lilium sp. (lily), which were isolated from specimens in Japan, Korea and Taiwan, were unable to support satellite RNA (satRNA) accumulation. In order to map the CMV sequences that are involved in satRNA support, HL-CMV (Japanese lily isolate), Y-CMV (ordinary strain) and Y-satellite RNA (Y-sat) were used as the source material. The pseudorecombinants between Y-CMV and HL-CMV revealed that RNA1 was essential for satRNA replication in lily. The results of chimeric constructs and various mutations showed that two amino acid residues (at positions 876 and 891) in the 1a protein were the determinants for the inability of HL-CMV to support a satRNA. Specifically, Thr at position 876 had a more pronounced effect than Met at position 891. Specific changes in RNA sequence were also detected in the 3′ terminus of Y-sat and these particular alterations allowed it to be supported by HL-CMV. It is believed that, through evolution, the adaptation of CMV to lily resulted in the introduction of amino acid changes in the 1a protein, changes that coincidentally affected the ability of lily CMV to support satRNAs.

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