Foliar Sprayed Green Zinc Oxide Nanoparticles Mitigate Drought-Induced Oxidative Stress in Tomato

This study explored the effectiveness of green zinc oxide nanoparticles (ZnO-NPs) foliar spray on tomato growth and oxidative stress relief under drought conditions. Tomato plant subjected to four water regimes (100, 75, 50, and 25% FC), and in the same while seedlings were sprayed with 25, 50, and 100 mg/L green ZnO-NPs. The results showed that tomato growth parameters reduced significantly by increasing drought stress levels, while ZnO-NPs enhanced plant growth under all studied drought levels. Out of three ZnO-NPs concentrations tested, 25 and 50 mg/L ZnO-NPs proved to be the optimum treatments for alleviating drought stress. They increased shoot and root biomass compared to untreated controls. Application of 25 and 50 mg/L ZnO-NPs enhanced shoot dry weight by about 2–2.5-fold, respectively, under severe drought conditions (25%) compared to ZnO-NPs untreated plants. The application of 25 and 50 mg/L green ZnO-NPs decreased the drought-induced oxidative stress as indicated by the reduction in malondialdehyde and hydrogen peroxide concentrations compared to untreated controls. While 100 mg/L ZnO-NPs further increased oxidative stress. The beneficial effects of ZnO-NPs were evident in the plants’ defensive state, in which the concentration of ascorbic acid, free phenols, and the activity of superoxide dismutase, catalase, and ascorbate peroxidase were maintained at higher levels compared to NPs-untreated plants. At severe drought conditions, 25 mg/L ZnO-NPs induced SOD, CAT, and APX activity by about 3.99-, 3.23-, and 2.82-fold of their corresponding controls, respectively. Likewise, at 25% FC, SOD, CAT, and APX activity increased with 50 mg/L ZnO-NPs by about 4.58-, 3.57-, and 3.25-fold consecutively compared with their respective controls. Therefore, foliar use of green ZnO-NPs at lower concentrations might be suggested as an efficient way for enhancing tomato tolerance to drought stress.

Study The Effect of Water Quality, Bio Booster (Zytonic F) and Nano NPK fertilizer on Tomato Growth and Yield

Bio stimulants are natural or synthetic substances that can be applied to seeds, plants, and soil. These substances cause changes in vital and structural processes in order to influence plant growth through improved tolerance to abiotic stresses and increase grain yield and quality. In addition, bio stimulants reduce the need for fertilizers so an experiment was carried to investigate the influence of water qualities with three levels (1.2, 8, and 10 ds.m-1 ) as a first factor and the bio stimulants combinations were used of the poultry litter, Bio–booster (Zytonic F), and nano NPK fertilizer as a second factor, to study the level of tomato sensitivity to salt stress due to the scarcity of salinity studies on a tomato plant in Iraq, the experiment was conducted based on Spilt - Plot system within the RCBD design, The results confirmed that the saline water caused a drop in the vegetative growth and yield. The fertilizer combinations had been producing significant differences compared to control, The treatments, F5 (poultry manure 30 tons. ha-1 + nano fertilizer) 2g.liter and F6 (Poultry manure 30ton. ha-1 + Zytonic-F 0.2g+10g of peat moos plant-1) obtained the best rates, in most of the traits of vegetative growth and yield, and it has been decreasing the harmful effect of saline water on tomato growth.

Optimizing Tomato Growth and Productivity Using Nitrogen and Irrigation Application Timing

Soil nutrients and water management practices according to the concept of 4R nutrient stewardship (right rate, right timing, right placement, and right source) can have significant benefits on crop productivity and reduce the negative effects of agricultural practices on the environment. Therefore, this present study evaluated the effects of nitrogen (N) application timing under different irrigation regimes on open-field, fresh-market tomato production in Florida. In this study, 2 N application timings applied at 25% pre-plant with 75% fertigation (BM), and 0 pre-plant with 100% fertigation (NB), were evaluated. The two N application methods were evaluates using three irrigation regimes: full irrigation (FI, 100% ETc), deficit irrigation (DI, 66% ETc), and regulated deficit irrigation (RDI, 66% ETc during the first 4 weeks after transplanting and 100% ETc afterward). The results showed that BM treatment significantly improved early-season tomato growth compared to NB treatment. The results also indicated that under RDI and DI irrigation conditions, tomato root length was lowest (average value of 13%) within the first 15 cm compared to 40% within 15–30 cm and 47% at 30–40 cm soil depths. Similar to plant growth, BM treatment significantly increased tomato yield (average valued 56.00 Mg ha−1) compared to the NB (average value 40.23 Mg ha−1). The application of DI throughout the growing season reduced tomato yield; however, there were no differences in yield under the RDI and FI irrigation regimes. Therefore, based on the results from this study, it can be concluded that, under Florida growing conditions, pre-plant N application is essential for tomato growth and productivity. Additionally, irrigation application using the RDI method could be successfully adopted in Florida tomato production for improved water savings without any negative effects on tomato growth and productivity.

Characterization of Phosphate Solubilizing Bacterial Endophytes and Plant Growth Promotion In Vitro and in Greenhouse

Phosphate is one of the most important nutrients for plant growth and development, and only 0.1% of the phosphate in soils is available to plants. Currently, the use of excess phosphate fertilizer has caused surface and ground water pollution and water eutrophication, resulting in algal blooms in lakes and oceans. Therefore, it is imperative to explore alternative ways to solve these problems for sustainable agricultural production and improvement of soil fertility, while protecting the environment. Microorganisms from the rhizosphere and within plants are able to solubilize insoluble soil phosphate, making it available to plants. Five high phosphate solubilizing bacteria from our bacterial endophyte library were chosen for this study and identified as Pantoea vagans IALR611, Pseudomonas psychrotolerans IALR632, Bacillus subtilis IALR1033, Bacillus safensis IALR1035 and Pantoea agglomerans IALR1325. All five bacteria significantly promoted tall fescue growth in vitro. Greenhouse experiments showed that IALR1325 significantly promoted pepper and tomato growth, and IALR632 was the best in promoting tomato growth. In addition, all these bacteria had extracellular acid phosphatase and phytase activities. One of the mechanisms for phosphate solubilization by bacteria is pH reduction caused by gluconic acid production. Our results indicate that P. agglomerans IALR1325 is a promising bacterium for future applications.

Effect of Organic Fertilizer on Tomato Growth and Production under Soil-less System

Soil-less system constitutes an efficient approach for the cultivation of tomato; however, organic liquid amendments are very limited under such systems. The current experiment aimed to evaluate the effects of Kurojiru (K), an organic liquid fertilizer and fulvic acid (FA) on the growth and production of tomato, Solanum lycopersicum L. cv. ‘Momotaro’. Recently, it is desirable to reduce the environmental impact and fertilizer cost by lowering the concentration of inorganic components in the culture medium. Therefore, we explored the effect of adding these organic fertilizers on the yield and quality of tomatoes by gradually reducing the phosphoric acid concentration in the culture medium (87, 58 and 29 mmol・pot-1・week-1). The whole experiment was conducted for 20 weeks (from seeding until harvesting). The plant biomass, tomato fruit weight and chlorophyll content were measured. The fresh weight (FW) of both root and shoot indicated a progress response according to phosphorus concentration in liquid media, in FA treatments. Total fresh weight was significantly higher in the treatment with K+FA than in the control. Additionally, the yield responded to the all treatments within the 58 mmol P. Especially, in this level of P the relative fruit weight was higher only under K application. The chlorophyll content responded K+FA treatment under a low P content (29 mmol), and to all of those in 58 mmol. These results indicate that Kurojiru has some positive effects on tomato growth in soil-less systems.

Ground-Based Hyperspectral Remote Sensing for Estimating Water Stress in Tomato Growth in Sandy Loam and Silty Loam Soils

Drought and water scarcity due to global warming, climate change, and social development have been the most death-defying threat to global agriculture production for the optimization of water and food security. Reflectance indices obtained by an Analytical Spectral Device (ASD) Spec 4 hyperspectral spectrometer from tomato growth in two soil texture types exposed to four water stress levels (70–100% FC, 60–70% FC, 50–60% FC, and 40–50% FC) was deployed to schedule irrigation and management of crops’ water stress. The treatments were replicated four times in a randomized complete block design (RCBD) in a 2 × 4 factorial experiment. Water stress treatments were monitored with Time Domain Reflectometer (TDR) every 12 h before and after irrigation to maintain soil water content at the desired (FC%). Soil electrical conductivity (Ec) was measured daily throughout the growth cycle of tomatoes in both soil types. Ec was revealing a strong correlation with water stress at R2 above 0.95 p < 0.001. Yield was measured at the end of the end of the growing season. The results revealed that yield had a high correlation with water stress at R2 = 0.9758 and 0.9816 p < 0.01 for sandy loam and silty loam soils, respectively. Leaf temperature (LT °C), relative leaf water content (RLWC), leaf chlorophyll content (LCC), Leaf area index (LAI), were measured at each growth stage at the same time spectral reflectance data were measured throughout the growth period. Spectral reflectance indices used were grouped into three: (1) greenness vegetative indices; (2) water overtone vegetation indices; (3) Photochemical Reflectance Index centered at 570 nm (PRI570), and normalized PRI (PRInorm). These reflectance indices were strongly correlated with all four water stress indicators and yield. The results revealed that NDVI, RDVI, WI, NDWI, NDWI1640, PRI570, and PRInorm were the most sensitive indices for estimating crop water stress at each growth stage in both sandy loam and silty loam soils at R2 above 0.35. This study recounts the depth of 858 to 1640 nm band absorption to water stress estimation, comparing it to other band depths to give an insight into the usefulness of ground-based hyperspectral reflectance indices for assessing crop water stress at different growth stages in different soil types.