ESI–MS method for in vitro investigation of skin penetration by copper–amino acid complexes: from an emulsion through a model membrane

2007 ◽  
Vol 388 (5-6) ◽  
pp. 1157-1163 ◽  
Author(s):  
Lena Mazurowska ◽  
Kinga Nowak-Buciak ◽  
Mirosław Mojski
Keyword(s):  
Amino Acid ◽  
Skin Penetration ◽  
Model Membrane ◽  
Esi Ms ◽  
Amino Acid Complexes ◽  
In Vitro Investigation

scholarly journals Characterization of zinc amino acid complexes for zinc delivery in vitro using Caco-2 cells and enterocytes from hiPSC

BioMetals ◽  
2017 ◽  
Vol 30 (5) ◽  
pp. 643-661 ◽  
Author(s):  
Ann Katrin Sauer ◽  
Stefanie Pfaender ◽  
Simone Hagmeyer ◽  
Laura Tarana ◽  
Ann-Kathrin Mattes ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Complexes

Pentacyclic triterpene carboxylic acids derivatives integrated piperazine-amino acid complexes for α-glucosidase inhibition in vitro

2021 ◽  
pp. 105212
Author(s):  
Jinxiang Huang ◽  
Xufeng Zang ◽  
Wuying Yang ◽  
Xiaoli Yin ◽  
Jianping Huang ◽  
...  
Keyword(s):  
Amino Acid ◽  
Carboxylic Acids ◽  
Pentacyclic Triterpene ◽  
Amino Acid Complexes

scholarly journals Evaluation the Effects of some Cobalt Amino Acids Complexes Using Leukocytes as in vitro Model for Cytotoxicity

1970 ◽  
Vol 66 (2) ◽  
Author(s):  
Andreea STĂNILĂ ◽  
Cornelia BRAICU
Keyword(s):  
Amino Acids ◽  
Lipid Peroxidation ◽  
Amino Acid ◽  
Whole Blood ◽  
In Vitro Model ◽  
Cobalt Complexes ◽  
Prooxidant Effect ◽  
Amino Acid Complexes ◽  
Vitro Model

It was evaluate the effect of amino acids complex we had used viable leukocytes readily obtained from sterile whole blood as an in vitro model for cytotoxicity. The end point for cytotoxicity evaluation was lactate dehidrogenase activity (LDH) and lipid peroxidation (MDA-TBA test). We tested 5 amino acid complexes: Co-leucine, Co-methionine, Co-valine, Co-hystidine, Co-phenylalanine at different concentrations. Some of the amino acids complexes determined the decreasing of LDH level after 8h, 24h and 48h which mean that these compounds have no cytotoxicity. Concerning the lipid peroxidation the lowest level were obtained for cobalt complexes with metionine, valine, leucine and hystidine at the concentrations of 2-0,2µM and for cobalt phenylalanine complexes for all concentrations especially after 24h and 48h. The higher levels of of lipid peroxidation were in the case of Copper-valine at 2µM and 20µM after 24h, Copper-hystidine at 20µM after 8h, 24h, 48h, and Co-leucine at 20µM after 48h, having a prooxidant effect.

Electron probe x-ray microanalysis and electron microscopy of amino acid absorption and transport by the small intestine: inhibition by chemical poisons and correlation to the elemental composition of the epithelial cells

1986 ◽  
Vol 44 ◽  
pp. 134-135
Author(s):  
A. J. Tousimis
Keyword(s):  
Small Intestine ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Elemental Composition ◽  
Isotope Labeling ◽  
Structural Components ◽  
X Ray ◽  
Human Small Intestine ◽  
Transport Studies

The elemental composition of amino acids is similar to that of the major structural components of the epithelial cells of the small intestine and other tissues. Therefore, their subcellular localization and concentration measurements are not possible by x-ray microanalysis. Radioactive isotope labeling: I131-tyrosine, Se75-methionine and S35-methionine have been successfully employed in numerous absorption and transport studies. The latter two have been utilized both in vitro and vivo, with similar results in the hamster and human small intestine. Non-radioactive Selenomethionine, since its absorption/transport behavior is assumed to be the same as that of Se75- methionine and S75-methionine could serve as a compound tracer for this amino acid.

In vitro investigation of Varizella zoster virus as an oncolytic virus in glioblastoma therapy

2008 ◽  
Vol 35 (S 01) ◽  
Author(s):  
H Leske ◽  
A Baiker ◽  
C Schichor ◽  
J.C Tonn ◽  
R Goldbrunner ◽  
...  
Keyword(s):  
Oncolytic Virus ◽  
In Vitro Investigation ◽  
Varizella Zoster Virus

Oxytocin analogues with non-coded amino acid residues in position 8: [8-Neopentylglycine]oxytocin and [8-cycloleucine]oxytocin

1987 ◽  
Vol 52 (9) ◽  
pp. 2317-2325 ◽  
Author(s):  
Jan Hlaváček ◽  
Jan Pospíšek ◽  
Jiřina Slaninová ◽  
Walter Y. Chan ◽  
Victor J. Hruby
Keyword(s):  
Amino Acid ◽  
Solid Phase Synthesis ◽  
Solid Phase ◽  
The Other ◽  
Amino Acid Residues ◽  
Phase Synthesis ◽  
Other Hand ◽  
Fragment Condensation

[8-Neopentylglycine]oxytocin (II) and [8-cycloleucine]oxytocin (III) were prepared by a combination of solid-phase synthesis and fragment condensation. Both analogues exhibited decreased uterotonic potency in vitro, each being about 15-30% that of oxytocin. Analogue II also displayed similarly decreased uterotonic potency in vivo and galactogogic potency. On the other hand, analogue III exhibited almost the same potency as oxytocin in the uterotonic assay in vivo and in the galactogogic assay.

Synthesis of Fragments of the Vasoactive Intestinal Peptide (VIP) and Analysis of Their Residual Biological Activities

1995 ◽  
Vol 60 (7) ◽  
pp. 1229-1235 ◽  
Author(s):  
Ivana Zoulíková ◽  
Ivan Svoboda ◽  
Jiří Velek ◽  
Václav Kašička ◽  
Jiřina Slaninová ◽  
...  
Keyword(s):  
Amino Acid ◽  
Biological Activities ◽  
Residual Activity ◽  
Detailed Examination ◽  
Amino Acid Residues ◽  
Linear Peptide ◽  
Zone Electrophoresis ◽  
Capillary Zone

The vasoactive intestinal (poly)peptide (VIP) is a linear peptide containing 28 amino acid residues, whose primary structure indicates a low metabolic stability. The following VIP fragments, as potential metabolites, and their analogues were prepared by synthesis on a solid: [His(Dnp)1]VIP(1-10), VIP(11-14), [D-Arg12]VIP(11-14), [Lys(Pac)15,21,Arg20]VIP(15-22), and VIP(23-28). After purification, the peptides were characterized by amino acid analysis, mass spectrometry, RP HPLC, and capillary zone electrophoresis. In some tests, detailed examination of the biological activity of the substances in vivo and in vitro gave evidence of a low, residual activity of some fragments, viz. a depressoric activity in vivo for [His(Dnp)1]VIP(1-10) and a stimulating activity for the release of α-amylase in vitro and in vivo for [Lys(Pac)15,21,Arg20]VIP(15-22) and VIP(23-28).

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