Evaluation the Effects of some Cobalt Amino Acids Complexes Using Leukocytes as in vitro Model for Cytotoxicity

It was evaluate the effect of amino acids complex we had used viable leukocytes readily obtained from sterile whole blood as an in vitro model for cytotoxicity. The end point for cytotoxicity evaluation was lactate dehidrogenase activity (LDH) and lipid peroxidation (MDA-TBA test). We tested 5 amino acid complexes: Co-leucine, Co-methionine, Co-valine, Co-hystidine, Co-phenylalanine at different concentrations. Some of the amino acids complexes determined the decreasing of LDH level after 8h, 24h and 48h which mean that these compounds have no cytotoxicity. Concerning the lipid peroxidation the lowest level were obtained for cobalt complexes with metionine, valine, leucine and hystidine at the concentrations of 2-0,2µM and for cobalt phenylalanine complexes for all concentrations especially after 24h and 48h. The higher levels of of lipid peroxidation were in the case of Copper-valine at 2µM and 20µM after 24h, Copper-hystidine at 20µM after 8h, 24h, 48h, and Co-leucine at 20µM after 48h, having a prooxidant effect.