Multiplex isotope dimethyl labeling of substrate peptides for high throughput kinase activity assay via quantitative MALDI MS

2014 ◽  
Vol 50 (90) ◽  
pp. 13960-13962 ◽  
Author(s):  
Zhenzhen Deng ◽  
Mingliang Ye ◽  
Yangyang Bian ◽  
Zheyi Liu ◽  
Fangjie Liu ◽  
...  
Keyword(s):  
High Throughput ◽  
Kinase Activity ◽  
Maldi Ms ◽  
Time Dependent ◽  
Activity Assay ◽  
Dimethyl Labeling ◽  
Labeling Approach ◽  
Kinase Activity Assay

A multiplex isotope dimethyl labeling approach allowed MALDI MS to monitor the time dependent consumption of substrates and generation of products in one spot.

A robust and quantitative assay platform for multiplexed, high throughput screening of protein kinase inhibitors

2016 ◽  
Vol 52 (81) ◽  
pp. 12112-12115 ◽  
Author(s):  
Jieon Lee ◽  
Il-Soo Park ◽  
Ginam Park ◽  
Kyukwang Cho ◽  
Hee-Sung Park ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Protein Kinase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
Kinase Inhibitors ◽  
Protein Kinase Inhibitors ◽  
Protein Kinase Activity ◽  
Activity Assay ◽  
Kinase Activity Assay

We present a new platform for multiplexed protein kinase activity assay using TiO2decorated graphene oxide (GO), which is applicable to high throughput inhibitor screening.

scholarly journals PhosphoFlowSeq – A high-throughput kinase activity assay for screening drug resistance mutations in EGFR

2021 ◽  
pp. 167210
Author(s):  
Anja Wagner ◽  
Magdalena Teufl ◽  
Lukas Gold ◽  
Manfred Lehner ◽  
Christian Obinger ◽  
...  
Keyword(s):  
Drug Resistance ◽  
High Throughput ◽  
Kinase Activity ◽  
Activity Assay ◽  
Resistance Mutations ◽  
Drug Resistance Mutations ◽  
Kinase Activity Assay

Homogeneous single-label tyrosine kinase activity assay for high throughput screening

2015 ◽  
Vol 897 ◽  
pp. 96-101 ◽  
Author(s):  
Natalia Tong-Ochoa ◽  
Kari Kopra ◽  
Markku Syrjänpää ◽  
Nicolas Legrand ◽  
Harri Härmä
Keyword(s):  
Tyrosine Kinase ◽  
High Throughput ◽  
High Throughput Screening ◽  
Kinase Activity ◽  
Activity Assay ◽  
Tyrosine Kinase Activity ◽  
Kinase Activity Assay

A rapid, multiplexed kinase activity assay using 8-plex iTRAQ labeling, SPE, and MALDI-TOF/TOF MS

The Analyst ◽  
2020 ◽  
Vol 145 (3) ◽  
pp. 992-1000
Author(s):  
Yu-Ching Liu ◽  
Fuu-Jen Tsai ◽  
Chao-Jung Chen
Keyword(s):  
Kinase Activity ◽  
Kinase Assay ◽  
Activity Assay ◽  
Peptide Substrates ◽  
Itraq Labeling ◽  
Cell Lysates ◽  
Maldi Tof ◽  
Kinase Activity Assay ◽  
Tof Ms

Synthesized peptide substrates with iTRAQ labeling has been used for in vitro kinase assay using cell lysates and detected by MALDI TOF/TOF MS.

Sign in / Sign up

Export Citation Format

Share Document