New extraction sorbent based on aptamers for the determination of ochratoxin A in red wine

2011 ◽  
Vol 400 (5) ◽  
pp. 1199-1207 ◽  
Author(s):  
Florence Chapuis-Hugon ◽  
Aude du Boisbaudry ◽  
Benjamin Madru ◽  
Valérie Pichon
Keyword(s):  
Ochratoxin A ◽  
Red Wine

scholarly journals Determination of Ochratoxin A in Wine and Beer by Immunoaffinity Column Cleanup and Liquid Chromatographic Analysis with Fluorometric Detection: Collaborative Study

2001 ◽  
Vol 84 (6) ◽  
pp. 1818-1827 ◽  
Author(s):  
Angelo Visconti ◽  
Michelangelo Pascale ◽  
Gianluca Centonze ◽  
E Anklam ◽  
A M Betbeder ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Red Wine ◽  
Collaborative Study ◽  
White Wine ◽  
Fluorometric Detection ◽  
Immunoaffinity Column ◽  
Relative Standard ◽  
Standard Deviations ◽  
Liquid Chromatographic

Abstract The accuracy, repeatability, and reproducibility characteristics of a liquid chromatographic method for the determination of ochratoxin A (OTA) in white wine, red wine, and beer were established in a collaborative study involving 18 laboratories in 10 countries. Blind duplicates of blank, spiked, and naturally contaminated materials at levels ranging from ≤0.01 to 3.00 ng/mL were analyzed. Wine and beer samples were diluted with a solution containing polyethylene glycol and sodium hydrogen carbonate, and the diluted samples were filtered and cleaned up on an immunoaffinity column. OTA was eluted with methanol and quantified by reversed-phase liquid chromatography with fluorometric detection. Average recoveries from white wine, red wine, and beer ranged from 88.2 to 105.4% (at spiking levels ranging from 0.1 to 2.0 ng/mL), from 84.3 to 93.1% (at spiking levels ranging from 0.2 to 3.0 ng/mL), and from 87.0 to 95.0% (at spiking levels ranging from 0.2 to 1.5 ng/mL), respectively. Relative standard deviations for within-laboratory repeatability (RSDr) ranged from 6.6 to 10.8% for white wine, from 6.5 to 10.8% for red wine, and from 4.7 to 16.5% for beer. Relative standard deviations for between-laboratories reproducibility (RSDR) ranged from 13.1 to 15.9% for white wine, from 11.9 to 13.6% for red wine, and from 15.2 to 26.1% for beer. HORRAT values were ≤0.4 for the 3 matrixes.

Determination of Ochratoxin A in Red Wine and Vinegar by Immunoaffinity High-Pressure Liquid Chromatography

2001 ◽  
Vol 64 (4) ◽  
pp. 533-537 ◽  
Author(s):  
P. MARKAKI ◽  
C. DELPONT-BINET ◽  
F. GROSSO ◽  
S. DRAGACCI
Keyword(s):  
Ochratoxin A ◽  
High Performance ◽  
Red Wine ◽  
Chromatographic Determination ◽  
High Performance Liquid Chromatographic ◽  
Balsamic Vinegar ◽  
Liquid Chromatographic Determination ◽  
Liquid Chromatographic ◽  
Good Agreement

A method is described for the determination of ochratoxin A (OTA) in red wine and vinegar using an acidic chloroform extraction, an immmunoaffinity clean-up step, and a high-performance liquid chromatographic determination with fluorescence detection. The detection limit was estimated at 0.002 μg/liter. The mean recovery factors were found at 91.3 and 96.6% for wine and vinegar, respectively. Thirty-one samples of red wine originating from Mediterranean sea countries and 15 samples of vinegar were examined for the presence of OTA. All red wine samples contained OTA. Seventy-two percent of these samples were found to be contaminated over 0.1 μg/liter. Among them, nine samples contained ochratoxin A in the range of 0.5 to 3.4 μg/liter, 12 samples in the range of 0.10 to 0.50 μg/liter (median: 0.176 μg/liter), and 9 samples in the range of 0.010 to 0.100 μg/liter (median: 0.041 μg/liter). All 15 vinegar samples showed the presence of OTA. The most contaminated ones were three balsamic vinegar samples containing 0.156 μg/liter, 0.102 μg/liter, and 0.252 μg/liter of OTA. In the remaining 12 samples, ochratoxin A levels ranged from 0.008 μg/liter to 0.046 μg/liter (median: 0.012 μg/liter). These data are in good agreement with the hypothesis that wine originating from Southern countries might contain significant OTA concentration and showed the possible occurrence of traces of OTA in vinegar.

Development of a very sensitive electrochemical magneto immunosensor for the direct determination of ochratoxin A in red wine

2012 ◽  
Vol 162 (1) ◽  
pp. 327-333 ◽  
Author(s):  
Patricio René Perrotta ◽  
Fernando Javier Arévalo ◽  
Nelio Roberto Vettorazzi ◽  
María Alicia Zón ◽  
Héctor Fernández
Keyword(s):  
Ochratoxin A ◽  
Red Wine ◽  
Direct Determination

Alternatives for sample pre-treatment and HPLC determination of Ochratoxin A in red wine using fluorescence detection

2010 ◽  
Vol 660 (1-2) ◽  
pp. 119-126 ◽  
Author(s):  
Catherine Tessini ◽  
Claudia Mardones ◽  
Dietrich von Baer ◽  
Mario Vega ◽  
Erika Herlitz ◽  
...  
Keyword(s):  
Ochratoxin A ◽  
Fluorescence Detection ◽  
Red Wine ◽  
Hplc Determination ◽  
Pre Treatment

Determination of ochratoxin A in wine by direct analysis in real time-tandem mass spectrometry

2017 ◽  
Vol 35 (2) ◽  
pp. 185 ◽  
Author(s):  
Xiaoming GONG ◽  
Ronghui MA ◽  
Hongtao WANG ◽  
Liqiang GUO ◽  
Kai LI ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Tandem Mass Spectrometry ◽  
Real Time ◽  
Ochratoxin A ◽  
Direct Analysis ◽  
Tandem Mass

Recent progress in determination of ochratoxin a in foods by chromatographic and mass spectrometry methods

2021 ◽  
pp. 1-32
Author(s):  
Xianjiang Li ◽  
Wen Ma ◽  
Zhiyong Ma ◽  
Qinghe Zhang ◽  
Hongmei Li
Keyword(s):  
Mass Spectrometry ◽  
Ochratoxin A ◽  
Recent Progress

scholarly journals Development of a novel UHPLC-MS/MS method for the determination of ochratoxin A in tea

Heliyon ◽  
2021 ◽  
Vol 7 (4) ◽  
pp. e06663
Author(s):  
Mariel Cina ◽  
María del Valle Ponce ◽  
Luis Dante Martinez ◽  
Soledad Cerutti
Keyword(s):  
Ochratoxin A

scholarly journals Comparison of Flow Injection-MS/MS and LC-MS/MS for the Determination of Ochratoxin A

Toxins ◽  
2021 ◽  
Vol 13 (8) ◽  
pp. 547
Author(s):  
Kai Zhang
Keyword(s):  
Ochratoxin A ◽  
Flow Injection ◽  
Internal Standard ◽  
Grape Juice ◽  
Tandem Mass ◽  
Triple Quadrupole ◽  
On Line ◽  
Red Grape Juice ◽  
Red Grape

Two methods for measuring ochratoxin A in corn, oat, and grape juice were developed and compared. Flow injection (FI) and on-line liquid chromatography (LC) performances were evaluated separately, with both methods using a triple quadrupole tandem mass spectrometer (MS/MS) for quantitation. Samples were fortified with 13C uniformly labeled ochratoxin A as the internal standard (13C-IS) and prepared by dilution and filtration, followed by FI- and LC-MS/MS analysis. For the LC-MS/MS method, which had a 10 min run time/sample, recoveries of ochratoxin A fortified at 1, 5, 20, and 100 ppb in corn, oat, red grape juice, and white grape juice ranged from 100% to 117% with RSDs < 9%. The analysis time of the FI-MS/MS method was <60 s/sample, however, the method could not detect ochratoxin A at the lowest fortification concentration, 1 ppb, in all tested matrix sources. At 5, 20, and 100 ppb, recoveries by FI-MS/MS ranged from 79 to 117% with RSDs < 15%. The FI-MS/MS method also had ~5× higher solvent and matrix-dependent instrument detection limits (0.12–0.35 ppb) compared to the LC-MS/MS method (0.02–0.06 ppb). In the analysis of incurred corn and oat samples, both methods generated comparable results within ±20% of reference values, however, the FI-MS/MS method failed to determine ochratoxin A in two incurred wheat flour samples due to co-eluted interferences due to the lack of chromatographic separation.

Sign in / Sign up

Export Citation Format

Share Document