Electrochemical nonenzymatic sensor for cholesterol determination in food

During the course of routine investigations it was noticed that an abnormal number of low serum cholesterol results were occurring in our laboratory, and that these results appeared to come from mentally retarded patients. An investigation was therefore carried out on over 1,400 patients in the Stoke Park Group of Hospitals. Whenever blood was taken for routine haematological examination a serum sample was also collected for cholesterol determination. A preliminary report of these findings was made (Eastham and Jancar, 1967).

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Method for Fully Automated Determination of Total Cholesterol in Blood Serum, Including Saponification and Extraction

Clinical Chemistry ◽

10.1093/clinchem/14.10.960 ◽

1968 ◽

Vol 14 (10) ◽

pp. 960-966 ◽

Cited By ~ 8

Author(s):

J van der Honing ◽

C C Saarloos ◽

J Stip

Keyword(s):

Carbon Tetrachloride ◽

Blood Serum ◽

Total Cholesterol ◽

Standard Error ◽

New Method ◽

Automated Method ◽

The Relationship ◽

Cholesterol Determination ◽

Automated Determination

Abstract A fully automated method has been developed for the determination of total cholesterol in blood serum, using the AutoAnalyzer system. According to the new method, based on the cholesterol determination of Abell et al. (1), the serum sample is saponified and subsequently extracted with carbon tetrachloride. After treatment with Liebermann-Burchard reagent, the amount of cholesterol is determined at 630 nm. The method can be used for free and esterified cholesterol because saponification is carried out. The relationship between the new method and that of Abell et al. is linear. The correlation coefficient is 0.98 and the standard error 1.5%. According to the new method, 30 samples can be analyzed per hour.

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Separation of serum high-density lipoprotein for cholesterol determination: ultracentrifugation vs precipitation with sodium phosphotungstate and magnesium chloride.

Clinical Chemistry ◽

10.1093/clinchem/27.6.838 ◽

1981 ◽

Vol 27 (6) ◽

pp. 838-841 ◽

Cited By ~ 122

Author(s):

L Seigler ◽

W T Wu

Keyword(s):

High Density Lipoprotein ◽

Magnesium Chloride ◽

Density Lipoprotein ◽

Cholesterol Content ◽

Reference Intervals ◽

High Density ◽

Selective Precipitation ◽

Serum High Density Lipoprotein ◽

Coefficients Of Variation ◽

Cholesterol Determination

Abstract We compared two methods for separation of serum high-density lipoprotein: selective precipitation with sodium phosphotungstate and magnesium chloride, and ultracentrifugation in sodium chloride solution (relative density 1.063). When the cholesterol content (determined enzymically with a centrifugal analyzer) of fractions obtained by each method was compared (ultracentrifugation = x), the correlation coefficient was 0.97; y = 1.01x - 11.2 mg/L; p less than 0.05; n = 54. The within-day and between-day coefficients of variation for this method were 1.1 and 4.0%, respectively. Reference intervals for high-density lipoprotein cholesterol in subpopulations categorized by age and sex were based on data obtained from volunteer blood donors.

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Correction for relative interferences in a cholesterol determination

Microchemical Journal ◽

10.1016/0026-265x(76)90068-0 ◽

1976 ◽

Vol 21 (4) ◽

pp. 485-495 ◽

Cited By ~ 4

Author(s):

M.T. Perlstein ◽

A. Manasterski ◽

R.J. Thibert ◽

B. Zak

Keyword(s):

Cholesterol Determination

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