Abstract
Microelectrode glucose biosensor based on three-dimensional hybrid nanoporous platinum/graphene oxide nanostructure was developed for rapid glucose detection of tomato and cucumber fruits. The nanostructure was fabricated by a two-step modification method on microelectrode for loading a larger amount of glucose oxidase. The nanoporous structure was prepared on the surface of the platinum microelectrode by electrochemical etching, and then graphene oxide was deposited on the prepared nanoporous electrode by electrochemical deposition. The nanoprorous platinum/graphene oxide nanostructure had the advantage of improving the effective surface area of the electrode and the loading quantity of glucose oxidase. As a result, the biosensor achieved a wide range of 0.1-20.0 mM in glucose detection, which had the ability to accurately detect the glucose content. It was found that the three-dimensional hybrid nanostructure on the electrode surface realized the rapid direct electrochemistry of glucose oxidase. Therefore, the biosensor achieved high glucose detection sensitivity (11.64 μA mM -1cm -2), low detection limit (13 μM) and rapid response time (reaching 95% steady-state response within 3 seconds), when calibrating in glucose standard solution. In agricultural application, the as-prepared biosensor was employed to detect the glucose concentration of tomato and cucumber samples. The results showed that the relative deviation of this method was less than 5% when compared with that of HPLC, implying high accuracy of the presented biosensor in glucose detection in plants.
Corrigendum to “Copper columnar nanostructures fabricated by glancing angle deposition as a robust and scalable method for high sensitive non-enzymatic glucose detection” [J. Appl. Surf. Sci. 518 (2020) 146182]