scholarly journals In-solution buffer-free digestion allows full-sequence coverage and complete characterization of post-translational modifications of the receptor-binding domain of SARS-CoV-2 in a single ESI–MS spectrum

2021 ◽  
Author(s):  
Luis Ariel Espinosa ◽  
Yassel Ramos ◽  
Ivan Andújar ◽  
Enso Onill Torres ◽  
Gleysin Cabrera ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Complete Characterization ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
Sequence Coverage ◽  
Post Translational Modifications ◽  
Full Sequence ◽  
Esi Ms

Receptor-binding domain of human α2-macroglobulin Expression, folding and biochemical characterization of a high-affinity recombinant derivative

FEBS Letters ◽  
1994 ◽  
Vol 344 (2-3) ◽  
pp. 242-246 ◽  
Author(s):  
Thor Las Holtet ◽  
Kåre Lehmann Nielsen ◽  
Michael Etzerodt ◽  
Søren Kragh Moestrup ◽  
Jørgen Gliemann ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Biochemical Characterization ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
High Affinity ◽  
Α2 Macroglobulin

scholarly journals An antibody-escape calculator for mutations to the SARS-CoV-2 receptor-binding domain

2021 ◽  
Author(s):  
Allison J Greaney ◽  
Tyler N Starr ◽  
Jesse D Bloom
Keyword(s):  
Receptor Binding ◽  
Polyclonal Antibody ◽  
Polyclonal Antibodies ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
Experimental Characterization ◽  
Antibody Recognition ◽  
Antigenic Properties ◽  
Interactive Version

A key goal of SARS-CoV-2 surveillance is to rapidly identify viral variants with mutations that reduce neutralization by polyclonal antibodies elicited by vaccination or infection. Unfortunately, direct experimental characterization of new viral variants lags their sequence-based identification. Here we help address this challenge by aggregating deep mutational scanning data into an "escape calculator" that estimates the antigenic effects of arbitrary combinations of mutations to the virus's spike receptor-binding domain (RBD). The calculator can be used to intuitively visualize how mutations impact polyclonal antibody recognition, and score the expected antigenic effect of combinations of mutations. These scores correlate with neutralization assays performed on SARS-CoV-2 variants, and emphasize the ominous antigenic properties of the recently described Omicron variant. An interactive version of the calculator is at https://jbloomlab.github.io/SARS2_RBD_Ab_escape_maps/escape-calc/, and we provide a Python module for batch processing.

scholarly journals Biochemical, Biophysical, and Functional Characterization of Bacterially Expressed and Refolded Receptor Binding Domain ofPlasmodium vivaxDuffy-binding Protein

2001 ◽  
Vol 276 (20) ◽  
pp. 17111-17116 ◽  
Author(s):  
Sanjay Singh ◽  
Kailash Pandey ◽  
Rana Chattopadhayay ◽  
Syed Shams Yazdani ◽  
Andrew Lynn ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Binding Protein ◽  
Functional Characterization ◽  
Binding Domain ◽  
Receptor Binding Domain

Evolutionary characterization of recent human H3N2 influenza A isolates from Japan and China: novel changes in the receptor binding domain

1996 ◽  
Vol 141 (7) ◽  
pp. 1349-1355 ◽  
Author(s):  
S. Lindstrom ◽  
S. Sugita ◽  
A. Endo ◽  
M. Ishida ◽  
P. Huang ◽  
...  
Keyword(s):  
Receptor Binding ◽  
Influenza A ◽  
Binding Domain ◽  
Receptor Binding Domain ◽  
H3n2 Influenza ◽  
Japan And China
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