Real-time loop-mediated isothermal amplification for the CaMV-35S promoter as a screening method for genetically modified organisms

2004 ◽  
Vol 218 (5) ◽  
pp. 496-500 ◽  
Author(s):  
Shiro Fukuta ◽  
Yuko Mizukami ◽  
Akira Ishida ◽  
Junichi Ueda ◽  
Mayumi Hasegawa ◽  
...  
Keyword(s):  
Real Time ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Screening Method ◽  
Isothermal Amplification ◽  
Camv 35S Promoter ◽  
35S Promoter ◽  
Camv 35S ◽  
Loop Mediated Isothermal Amplification ◽  
Time Loop

PCR-ELISA for the CaMV-35S promoter as a screening method for genetically modified Roundup Ready soybeans

2001 ◽  
Vol 213 (4-5) ◽  
pp. 366-371 ◽  
Author(s):  
Hans-Josef Brunnert ◽  
Friedrich Spener ◽  
Torsten Börchers
Keyword(s):  
Genetically Modified ◽  
Screening Method ◽  
Camv 35S Promoter ◽  
35S Promoter ◽  
Roundup Ready ◽  
Camv 35S

Event-specific analytical methods for six genetically modified maize events using visual and real-time loop-mediated isothermal amplification

Food Control ◽  
2015 ◽  
Vol 55 ◽  
pp. 18-30 ◽  
Author(s):  
Rajesh K. Bhoge ◽  
Rashmi Chhabra ◽  
Gurinderjit Randhawa ◽  
Muthukrishnan Sathiyabama ◽  
Monika Singh
Keyword(s):  
Real Time ◽  
Analytical Methods ◽  
Genetically Modified ◽  
Isothermal Amplification ◽  
Genetically Modified Maize ◽  
Loop Mediated Isothermal Amplification ◽  
Time Loop

scholarly journals Simultaneous multiple target detection in real-time loop-mediated isothermal amplification

BioTechniques ◽  
2012 ◽  
Vol 53 (2) ◽  
pp. 81-89 ◽  
Author(s):  
Nathan A. Tanner ◽  
Yinhua Zhang ◽  
Thomas C. Evans
Keyword(s):  
Real Time ◽  
Target Detection ◽  
Isothermal Amplification ◽  
Multiple Target ◽  
Loop Mediated Isothermal Amplification ◽  
Time Loop

scholarly journals Quantification of the 35S Promoter in DNA Extracts from Genetically Modified Organisms Using Real-Time Polymerase Chain Reaction and Specificity Assessment on Various Genetically Modified Organisms, Part I: Operating Procedure

2005 ◽  
Vol 88 (2) ◽  
pp. 547-557 ◽  
Author(s):  
Sophie Fernandez ◽  
Chrystèle Charles-Delobel ◽  
Angèle Geldreich ◽  
Georges Berthier ◽  
Francine Boyer ◽  
...  
Keyword(s):  
Mosaic Virus ◽  
Real Time ◽  
Genetically Modified Organisms ◽  
Limit Of Detection ◽  
Genetically Modified ◽  
Collaborative Study ◽  
Cauliflower Mosaic Virus ◽  
Performance Criteria ◽  
35S Promoter ◽  
Conserved Sequence

Abstract A highly sensitive quantitative real-time assay targeted on the 35S promoter of a commercial genetically modified organism (GMO) was characterized (sF/sR primers) and developed for an ABI Prism® 7700 Sequence Detection System and TaqMan® chemistry. The specificity assessment and performance criteria of sF/sR assay were compared to other P35S-targeted published assays. sF/sR primers amplified a 79 base pair DNA sequence located in a part of P35S that is highly conserved among many caulimovirus strains, i.e., this consensus part of CaMV P35S is likely to be present in many GM events. According to the experimental conditions, the absolute limit of detection for Bt176 corn was estimated between 0.2 and 2 copies of equivalent genome (CEG). The limit of quantification was reached below 0.1% Bt176 content. A Cauliflower Mosaic Virus control (CaMV) qualitative assay targeted on the ORF III of the viral genome was also used as a control (primers 3F/3R) to assess the presence of CaMV in plant-derived products. The specificity of this test was assessed on various CaMV strains, including the Figwort Mosaic Virus (FMV) and solanaceous CaMV strains. Considering the performance of sF/sR quantification test, the highly conserved sequence, and the small size of the amplicon, this assay was tested in a collaborative study in order to be proposed as an international standard.

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