The Effects of Bone Remodeling Inhibition by Alendronate on Three-Dimensional Microarchitecture of Subchondral Bone Tissues in Guinea Pig Primary Osteoarthrosis

Burgos and Wislocki described changes in the mucosa of the guinea pig uterus, cervix and vagina during the estrous cycle investigated by transmission electron microscopy. More recently, Moghissi and Reame reported the effects of progestational agents on the human female reproductive tract. They found drooping and shortening of cilia in norgestrel and norethindrone- treated endometria. To the best of our knowledge, no studies concerning the effects of mestranol and norethindrone given concurrently on the three-dimensional surface features on the uterine mucosa of the guinea pig have been reported. The purpose of this study was to determine the effect of mestranol and norethindrone on surface ultrastructure of guinea pig uterus by SEM.Seventy eight animals were used in this study. They were allocated into two groups. Group 1 (20 animals) was injected intramuscularly 0.1 ml vegetable oil and served as controls.

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Early inhibition of subchondral bone remodeling slows load‐induced post‐traumatic osteoarthritis development in mice

Journal of Bone and Mineral Research ◽

10.1002/jbmr.4397 ◽

2021 ◽

Author(s):

Sophia N. Ziemian ◽

Ana Witkowski ◽

Timothy M Wright ◽

Miguel Otero ◽

Marjolein C. H. Meulen

Keyword(s):

Bone Remodeling ◽

Subchondral Bone ◽

Post Traumatic ◽

Early Inhibition

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Computer-aided Three-dimensional Reconstruction of Guinea Pig Cochlear Aqueduct

Acta Oto-Laryngologica ◽

10.3109/00016489109138430 ◽

1991 ◽

Vol 111 (5) ◽

pp. 917-920 ◽

Cited By ~ 5

Author(s):

Ryuzo Toriya ◽

Toshio Arima ◽

Akio Kuraoka ◽

Takuya Uemura

Keyword(s):

Guinea Pig ◽

Three Dimensional ◽

Three Dimensional Reconstruction ◽

Cochlear Aqueduct ◽

Computer Aided ◽

Dimensional Reconstruction

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Relationship between articular cartilage damage and subchondral bone properties and meniscal ossification in the Dunkin Hartley guinea pig model of osteoarthritis

Scandinavian Journal of Rheumatology ◽

10.3109/03009742.2011.571218 ◽

2011 ◽

Vol 40 (5) ◽

pp. 391-399 ◽

Cited By ~ 19

Author(s):

JS Thomsen ◽

TS Straarup ◽

CC Danielsen ◽

H Oxlund ◽

A Brüel

Keyword(s):

Articular Cartilage ◽

Guinea Pig ◽

Subchondral Bone ◽

Cartilage Damage ◽

Pig Model ◽

Articular Cartilage Damage ◽

Bone Properties ◽

Guinea Pig Model

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Subchondral Bone Remodeling: A Therapeutic Target for Osteoarthritis

Frontiers in Cell and Developmental Biology ◽

10.3389/fcell.2020.607764 ◽

2021 ◽

Vol 8 ◽

Author(s):

Xiaobo Zhu ◽

Yau Tsz Chan ◽

Patrick S. H. Yung ◽

Rocky S. Tuan ◽

Yangzi Jiang

Keyword(s):

Extracellular Matrix ◽

Signaling Pathways ◽

Bone Remodeling ◽

Molecular Mechanism ◽

Subchondral Bone ◽

Therapeutic Target ◽

Therapeutic Targets ◽

Regenerative Therapies

There is emerging awareness that subchondral bone remodeling plays an important role in the development of osteoarthritis (OA). This review presents recent investigations on the cellular and molecular mechanism of subchondral bone remodeling, and summarizes the current interventions and potential therapeutic targets related to OA subchondral bone remodeling. The first part of this review covers key cells and molecular mediators involved in subchondral bone remodeling (osteoclasts, osteoblasts, osteocytes, bone extracellular matrix, vascularization, nerve innervation, and related signaling pathways). The second part of this review describes candidate treatments for OA subchondral bone remodeling, including the use of bone-acting reagents and the application of regenerative therapies. Currently available clinical OA therapies and known responses in subchondral bone remodeling are summarized as a basis for the investigation of potential therapeutic mediators.

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Bone remodeling using a three-dimensional chitosan - hydroxyapatite scaffold seeded with hypoxic conditioned human amnion mesenchymal stem cells

Dental Journal (Majalah Kedokteran Gigi) ◽

10.20473/j.djmkg.v54.i2.p68-73 ◽

2021 ◽

Vol 54 (2) ◽

pp. 68

Author(s):

Michael Josef Kridanto Kamadjaja

Keyword(s):

Stem Cells ◽

Mesenchymal Stem Cells ◽

Bone Remodeling ◽

Three Dimensional ◽

Wistar Rat ◽

Control Group ◽

Human Amnion ◽

Morphogenetic Protein ◽

Hydroxyapatite Scaffold ◽

Endothelial Growth Factor

Background: Bone regeneration studies involving the use of chitosan–hydroxyapatite (Ch-HA) scaffold seeded with human amnion mesenchymal stem cells (hAMSCs) have largely incorporated tissue engineering experiments. However, at the time of writing, the results of such investigations remain unclear. Purpose: The aim of this study was to determine the osteogenic differentiation of the scaffold Ch-HA that is seeded with hAMSCs in the regeneration of calvaria bone defect. Methods: Ch-HA scaffold of 5 mm diameter and 2 mm height was created by lyophilisation and desalination method. hAMSCs were cultured in hypoxia environment (5% oxygen, 10% carbon dioxide, 15% nitrogen) and seeded on the scaffold. Twenty male Wistar rat subjects (8 – 10 weeks, 200 - 250 grams) were randomly divided into two groups: control and hydroxyapatite scaffold (HAS). Defects (similar size to scaffold size) were created in the calvaria bone of the all-group subjects, but a scaffold was subsequently implanted only in the treatment group members. Control group left without treatment. After observation lasting 1 and 8 weeks, the subjects were examined histologically and immunohistochemically. Statistical analysis was done using ANOVA test. Results: Angiogenesis; expression of vascular endothelial growth factor; bone morphogenetic protein; RunX-2; alkaline phosphatase; type-1 collagen; osteocalcin and the area of new trabecular bone were all significantly greater in the HAS group compared to the control group. Conclusion: The three-dimensional Ch-HA scaffold seeded with hypoxic hAMSCs induced bone remodeling in calvaria defect according to the expression of the osteogenic and angiogenic marker.

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