Isolated bacteriocyte cell suspensions from the hydrothermal-vent tubeworm Riftia pachyptila, a potent tool for cellular physiology in a chemoautotrophic symbiosis

2003 ◽  
Vol 142 (1) ◽  
pp. 141-151 ◽  
Author(s):  
M.-C. De Cian ◽  
A. Andersen ◽  
J.-Y. Toullec ◽  
I. Biegala ◽  
J.-C. Caprais ◽  
...  
Keyword(s):  
Hydrothermal Vent ◽  
Cell Suspensions ◽  
Riftia Pachyptila ◽  
Cellular Physiology ◽  
Chemoautotrophic Symbiosis

scholarly journals Polyclonal symbiont populations in hydrothermal vent tubeworms and the environment

2019 ◽  
Vol 286 (1896) ◽  
pp. 20181281 ◽  
Author(s):  
Julia Polzin ◽  
Philip Arevalo ◽  
Thomas Nussbaumer ◽  
Martin F. Polz ◽  
Monika Bright
Keyword(s):  
Hydrothermal Vent ◽  
Low Frequency ◽  
Gene Sequencing ◽  
Housekeeping Genes ◽  
Partner Choice ◽  
Sequence Variability ◽  
Riftia Pachyptila ◽  
Free Living ◽  
High Coverage ◽  
Dominant Genotype

Horizontally transmitted symbioses usually house multiple and variable symbiont genotypes that are acquired from a much more diverse environmental pool via partner choice mechanisms. However, in the deep-sea hydrothermal vent tubeworm Riftia pachyptila (Vestimentifera, Siboglinidae), it has been suggested that the Candidatus Endoriftia persephone symbiont is monoclonal. Here, we show with high-coverage metagenomics that adult R. pachyptila house a polyclonal symbiont population consisting of one dominant and several low-frequency variants. This dominance of one genotype is confirmed by multilocus gene sequencing of amplified housekeeping genes in a broad range of host individuals where three out of four loci ( atpA , uvrD and recA ) revealed no genomic differences, while one locus ( gyrB ) was more diverse in adults than in juveniles. We also analysed a metagenome of free-living Endoriftia and found that the free-living population showed greater sequence variability than the host-associated population. Most juveniles and adults shared a specific dominant genotype, while other genotypes can dominate in few individuals. We suggest that although generally permissive, partner choice is selective enough to restrict uptake of some genotypes present in the environment.

ATP sulfurylase from trophosome tissue of Riftia pachyptila (hydrothermal vent tube worm)

1991 ◽  
Vol 290 (1) ◽  
pp. 66-78 ◽  
Author(s):  
Franco Renosto ◽  
Robert L. Martin ◽  
Jeffrey L. Borrell ◽  
Douglas C. Nelson ◽  
Irwin H. Segel
Keyword(s):  
Hydrothermal Vent ◽  
Riftia Pachyptila ◽  
Atp Sulfurylase ◽  
Vent Tube

Unusual carbon dioxide-combining properties of body fluids in the hydrothermal vent tubeworm Riftia pachyptila

1994 ◽  
Vol 41 (10) ◽  
pp. 1447-1456 ◽  
Author(s):  
André Toulmond ◽  
François H. Lallier ◽  
Jacques de Frescheville ◽  
James J. Childress ◽  
Raymond Lee ◽  
...  
Keyword(s):  
Carbon Dioxide ◽  
Hydrothermal Vent ◽  
Body Fluids ◽  
Riftia Pachyptila

scholarly journals Nitrate respiration in the hydrothermal vent tubeworm Riftia pachyptila

Nature ◽  
1993 ◽  
Vol 366 (6453) ◽  
pp. 338-340 ◽  
Author(s):  
U. Hentschel ◽  
H. Felbeck
Keyword(s):  
Hydrothermal Vent ◽  
Nitrate Respiration ◽  
Riftia Pachyptila

scholarly journals Bacterial symbiont subpopulations have different roles in a deep-sea symbiosis

eLife ◽  
2021 ◽  
Vol 10 ◽  
Author(s):  
Tjorven Hinzke ◽  
Manuel Kleiner ◽  
Mareike Meister ◽  
Rabea Schlüter ◽  
Christian Hentschker ◽  
...  
Keyword(s):  
Deep Sea ◽  
Hydrothermal Vent ◽  
Carbon Fixation ◽  
Density Gradient Centrifugation ◽  
Gradient Centrifugation ◽  
Bacterial Symbiont ◽  
Riftia Pachyptila ◽  
Physiological Diversity ◽  
Host Interaction ◽  
Sulfur Oxidizing

The hydrothermal vent tubeworm Riftia pachyptila hosts a single 16S rRNA phylotype of intracellular sulfur-oxidizing symbionts, which vary considerably in cell morphology and exhibit a remarkable degree of physiological diversity and redundancy, even in the same host. To elucidate whether multiple metabolic routes are employed in the same cells or rather in distinct symbiont subpopulations, we enriched symbionts according to cell size by density gradient centrifugation. Metaproteomic analysis, microscopy, and flow cytometry strongly suggest that Riftia symbiont cells of different sizes represent metabolically dissimilar stages of a physiological differentiation process: While small symbionts actively divide and may establish cellular symbiont-host interaction, large symbionts apparently do not divide, but still replicate DNA, leading to DNA endoreduplication. Moreover, in large symbionts, carbon fixation and biomass production seem to be metabolic priorities. We propose that this division of labor between smaller and larger symbionts benefits the productivity of the symbiosis as a whole.

scholarly journals Epifaunal community structure associated with Riftia pachyptila aggregations in chemically different hydrothermal vent habitats

2005 ◽  
Vol 305 ◽  
pp. 67-77 ◽  
Author(s):  
B Govenar ◽  
N Le Bris ◽  
S Gollner ◽  
J Glanville ◽  
AB Aperghis ◽  
...  
Keyword(s):  
Community Structure ◽  
Hydrothermal Vent ◽  
Riftia Pachyptila ◽  
Epifaunal Community
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