Intensity-based energy transfer measurements in digital imaging microscopy

1998 ◽  
Vol 27 (4) ◽  
pp. 377-389 ◽  
Author(s):  
Péter Nagy ◽  
György Vámosi ◽  
Andrea Bodnár ◽  
Stephen J. Lockett ◽  
János Szöllősi
Keyword(s):  
Energy Transfer ◽  
Digital Imaging ◽  
Digital Imaging Microscopy

Fluorescence digital imaging microscopy in cell biology

Science ◽  
1985 ◽  
Vol 230 (4723) ◽  
pp. 247-256 ◽  
Author(s):  
D. Arndt-Jovin ◽  
M Robert-Nicoud ◽  
S. Kaufman ◽  
T. Jovin
Keyword(s):  
Digital Imaging ◽  
Cell Biology ◽  
Digital Imaging Microscopy

scholarly journals Fluorescence ratio measurements of double-labeled probes for multiple in situ hybridization by digital imaging microscopy

Cytometry ◽  
1992 ◽  
Vol 13 (8) ◽  
pp. 839-845 ◽  
Author(s):  
P. M. Nederlof ◽  
S. van der Flier ◽  
J. Vrolijk ◽  
H. J. Tanke ◽  
A. K. Raap
Keyword(s):  
In Situ Hybridization ◽  
Digital Imaging ◽  
Fluorescence Ratio ◽  
Digital Imaging Microscopy

scholarly journals Microtubule dynamics and chromosome motion visualized in living anaphase cells.

1988 ◽  
Vol 106 (4) ◽  
pp. 1185-1192 ◽  
Author(s):  
G J Gorbsky ◽  
P J Sammak ◽  
G G Borisy
Keyword(s):  
Chromosome Segregation ◽  
Digital Imaging ◽  
Living Cells ◽  
Microtubule Dynamics ◽  
Chromosome Movement ◽  
Animal Cells ◽  
Digital Imaging Microscopy ◽  
Opposite Pole ◽  
Anaphase B ◽  
Chromosome Motion

Chromosome segregation in most animal cells is brought about through two events: the movement of the chromosomes to the poles (anaphase A) and the movement of the poles away from each other (anaphase B). Essential to an understanding of the mechanism of mitosis is information on the relative movements of components of the spindle and identification of sites of subunit loss from shortening microtubules. Through use of tubulin derivatized with X-rhodamine, photobleaching, and digital imaging microscopy of living cells, we directly determined the relative movements of poles, chromosomes, and a marked domain on kinetochore fibers during anaphase. During chromosome movement and pole-pole separation, the marked domain did not move significantly with respect to the near pole. Therefore, the kinetochore microtubules were shortened by the loss of subunits at the kinetochore, although a small amount of subunit loss elsewhere was not excluded. In anaphase A, chromosomes moved on kinetochore microtubules that remained stationary with respect to the near pole. In anaphase B, the kinetochore fiber microtubules accompanied the near pole in its movement away from the opposite pole. These results eliminate models of anaphase in which microtubules are thought to be traction elements that are drawn to and depolymerized at the pole. Our results are compatible with models of anaphase in which the kinetochore fiber microtubules remain anchored at the pole and in which microtubule dynamics are centered at the kinetochore.

Characterization of Topotecan-Mediated Redistribution of DNA Topoisomerase I by Digital Imaging Microscopy

1998 ◽  
Vol 241 (2) ◽  
pp. 332-339 ◽  
Author(s):  
Randy M. Wadkins ◽  
Mary K. Danks ◽  
Lisa Horowitz ◽  
Sharyn D. Baker
Keyword(s):  
Digital Imaging ◽  
Topoisomerase I ◽  
Dna Topoisomerase I ◽  
Dna Topoisomerase ◽  
Digital Imaging Microscopy

Altered reactivity of hemolysate-treated cultured smooth-muscle cells from rabbit basilar artery determined by digital imaging microscopy

1991 ◽  
Vol 75 (1) ◽  
pp. 82-90 ◽  
Author(s):  
Yoshihiro Takanashi ◽  
Kazuhiko Fujitsu ◽  
Satoshi Fujii ◽  
Takeo Kuwabara
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Digital Imaging ◽  
Muscle Cells ◽  
Blocking Agent ◽  
Interference Microscopy ◽  
Morphological Alteration ◽  
Ultrastructural Changes ◽  
Digital Imaging Microscopy ◽  
Basilar Arteries

✓ During culture, smooth-muscle cells obtained from rabbit basilar arteries were examined for contractile activity by means of differential interference microscopy with a video analysis system (digital imaging microscopy system). This system proved useful for observing the contraction and ultrastructural changes of the living cells. Hemolysate-treated cells showed augmented responses to 5-hydroxytryptamine and leukotriene C4, but not to KCl. This augmented response diminished gradually during the culture period. Both a phospholipase C blocking agent, 2-nitro-4-carboxyphenyl-n,n-diphenylcarbamate (NCDC), and a myosin light chain kinase blocking agent, 1-(5-chloronaphthalenesulfonyl)-1 H-hexahydro-1,4-diazepine (ML-9), suppressed this augmented response. Protein kinase C activity of the cells, as measured by Western blot analysis, did not increase during the period of culture with hemolysate. The results obtained suggest that hemolysate had the following effects on the cells: 1) acute but gradual contraction of the cells; 2) augmentation of cellular responses to vasoactive agents; and 3) progressive contraction and morphological alteration of the cells. Possible mechanisms by which hemolysate exerts these effects are discussed, taking into consideration the interrelationship between these effects.

Measurement of internal pH in the coccolithophoreEmiliania huxleyi using 2?,7?-bis-(2-carboxyethyl)-5(and-6)carboxyfluorescein acetoxymethylester and digital imaging microscopy

Planta ◽  
1989 ◽  
Vol 178 (4) ◽  
pp. 443-449 ◽  
Author(s):  
G. K. Dixon ◽  
C. Brownlee ◽  
M. J. Merrett
Keyword(s):  
Digital Imaging ◽  
Digital Imaging Microscopy ◽  
Internal Ph

Digital imaging microscopy: the marriage of spectroscopy and the solid state CCD camera

1991 ◽  
Author(s):  
Thomas M. Jovin ◽  
Donna J. Arndt-Jovin
Keyword(s):  
Solid State ◽  
Digital Imaging ◽  
Ccd Camera ◽  
Digital Imaging Microscopy

scholarly journals Analysis of calcium transient and cell contraction in a single cardiomuscular cell by digital imaging microscopy with indo-1

1990 ◽  
Vol 52 ◽  
pp. 299
Author(s):  
Kazuhiko Tamura ◽  
Shohshin Yoshida ◽  
Takeshi Iwai ◽  
Ichiro Watanabe ◽  
Yutaka Sugawara
Keyword(s):  
Digital Imaging ◽  
Calcium Transient ◽  
Cell Contraction ◽  
Digital Imaging Microscopy
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